Cell Signaling Technology

Product Pathways - Transcription Factors

PAX3 Antibody #12412

sc-7748  

No. Size Price
12412S 100 µl ( 10 western blots ) ¥3,100.00 现货查询 购买询价
12412 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human, Transfected Only 62 Rabbit

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting,

Homology

Species predicted to react based on 100% sequence homology: Mouse, Rat, Monkey,

Specificity / Sensitivity

PAX3 Antibody recognizes transfected levels of total PAX3 protein.

PAX3抗体可以识别转染的总PAX3蛋白。

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human PAX3 protein. Antibodies are purified by protein A and peptide affinity chromatography.

此多抗由合成肽段免疫动物产生,该肽段与人PAX3蛋白羧基末端氨基酸残基序列一致。抗体由蛋白A和肽段亲和层析技术纯化得到。

Western Blotting

Western Blotting

Western blot analysis of extracts from 293 cells, mock transfected (-) or transfected with a construct expressing Myc/DDK-tagged full-length human PAX3 (hPAX3-Myc/DDK; +), using PAX3 Antibody (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower).

空转染对照(-)或转染了表达含有Myc/DDK标签的全长人PAX3 (hPAX3-Myc/DDK; +)的293细胞提取物,使用PAX3抗体(上)或β-Actin (D6A8) Rabbit mAb 兔单抗#8457(下)进行western blot分析。

Background

Paired box (PAX) proteins are a family of transcription factors that play important and diverse roles in animal development (1). Nine PAX proteins (PAX1-9) have been described in humans and other mammals. They are defined by the presence of an amino-terminal "paired" domain, consisting of two helix-turn-helix motifs, with DNA binding activity (2). PAX proteins are classified into four structurally distinct subgroups (I-IV) based on the absence or presence of a carboxy-terminal homeodomain and a central octapeptide region. Subgroup I (PAX1 and 9) contains the octapeptide but lacks the homeodomain; subgroup II (PAX2, 5, and 8) contains the octapeptide and a truncated homeodomain; subgroup III (PAX3 and 7) contains the octapeptide and a complete homeodomain; and subgroup IV (PAX4 and 6) contains a complete homeodomain but lacks the octapeptide region (2). PAX proteins play critically important roles in development by regulating transcriptional networks responsible for embryonic patterning and organogenesis (3); a subset of PAX proteins also maintain functional importance during postnatal development (4). Research studies have implicated genetic mutations that result in aberrant expression of PAX genes in a number of cancer subtypes (1-3), with members of subgroups II and III identified as potential mediators of tumor progression (2).

Paired box (PAX)蛋白是一组在动物发育过程中发挥了重要的及多样性作用的转录因子家族(1)。人和其他哺乳动物中发现了9个PAX蛋白(PAX1-9)。它们都含有氨基端“配对”结构域,包括了2个具有DNA结合活性的螺旋-转角-螺旋基序(2)。PAX蛋白,基于一个羧基末端同源域和中心八肽区域的存在与否可以根据不同的结构分为4个不同的亚型(I-IV)。亚型I (PAX1 和9)包括八肽但是缺乏同源域;亚型II (PAX2, 5, 和 8)包括了八肽和被截短的同源域;亚型III (PAX3 和7)包括了八肽和完整的同源域;和亚型IV (PAX4 和6)包括了一个完整的同源域但是缺乏八肽结构(2)。PAX蛋白在发育过程中发挥了重要作用调控了胚胎模式和器官发生过程中的转录调控网络(3);PAX蛋白的一组亚型还在产后发育过程中发挥重要功能(4)。研究提示基因突变引发的PAX基因表达异常与多种癌症相关(1-3),部分亚型II和III被认为是癌症进程中可能的介导因子(2)。

  1. Lang, D. et al. (2007) Biochem Pharmacol 73, 1-14.
  2. Robson, E.J. et al. (2006) Nat Rev Cancer 6, 52-62.
  3. Wang, Q. et al. (2008) J Cell Mol Med 12, 2281-94.
  4. Blake, J.A. et al. (2008) Dev Dyn 237, 2791-803.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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