Cell Signaling Technology

Product Pathways - MAPK Signaling

SOS1 (D3T7T) Rabbit mAb #12409

Sevenless  

No. Size Price
12409S 100 µl ( 10 western blots ) ¥3,100.00 现货查询 购买询价
12409 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat,Hamster,Monkey, Endogenous 152 Rabbit IgG

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting,

Specificity / Sensitivity

SOS1 (D3T7T) Rabbit mAb recognizes endogenous levels of total SOS1 protein.

SOS1 (D3T7T)Rabbit mAb兔单抗可以识别内源性总的SOS1蛋白。

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro1250 of human SOS1 protein.

单克隆抗体由合成肽段免疫动物产生,该肽段与人SOS1蛋白Pro1250附近氨基酸一致。

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using SOS1 (D3T7T) Rabbit mAb.使用SOS1 (D3T7T)Rabbit mAB对多种细胞提取物进行western blot分析。

Background

Son of sevenless (SOS) was first identified in Drosophila as a guanine nucleotide exchange factor (GEF) for Ras acting downstream of the Sevenless receptor (1). Two closely related homologs of Drosophila SOS are found in mammalian cells: SOS1 and SOS2 (2). SOS1 consists of histone folds, Dbl (DH) and pleckstrin (PH) homology domains, a Ras exchange motif (REM), and Cdc25 homology and polyproline domains (3). SOS1 binds to GRB2, NCK, and other adaptor proteins, and plays an important role in ERK activation downstream of protein tyrosine kinase receptor (RTK). Research studies have identified mutations in the corresponding SOS1 gene of patients with Noonan syndrome, a developmental disorder characterized by short stature, facial dysmorphia, and congenital heart defects (4,5).

交换因子(SOS)是果蝇中最早发现的Ras鸟苷酸交换因子,作为Sevenless 受体的下游(1)。哺乳动物细胞中发现了2个果蝇SOS的同源物:SOS1和SOS2(2)。SOS1含有组氨酸折叠,Dbl(DH)和pleckstrin(PH)同源结构域,一个Ras交换基序(REM)和Cdc25同源物和多脯氨酸结构域(3)。SOS1和GRB2、NCK和其他受体蛋白结合,在ERK激活下游受体酪氨酸激酶受体(RTK)的过程中发挥了重要作用。研究发现了Noonan综合征病人的SOS1基因发生突变,这是一个以身材矮小、面部变形和先天性心脏病为特征的疾病(4,5)。

  1. Rogge, R.D. et al. (1991) Cell 64, 39-48.
  2. Nimnual, A. and Bar-Sagi, D. (2002) Sci STKE 2002, pe36.
  3. Chardin, P. et al. (1993) Science 260, 1338-43.
  4. Tartaglia, M. et al. (2007) Nat Genet 39, 75-9.
  5. Roberts, A.E. et al. (2007) Nat Genet 39, 70-4.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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