Cell Signaling Technology

Product Pathways - PI3K / Akt Signaling

PI3 Kinase Class II α (D3Q5B) Rabbit mAb #12402

No. Size Price
12402S 100 µl ( 10 western blots ) ¥3,100.00 现货查询 购买询价
12402 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat,Monkey, Endogenous 180 Rabbit IgG
IP 1:50

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation,

Specificity / Sensitivity

PI3 Kinase Class II α (D3Q5B) Rabbit mAb recognizes endogenous levels of total PI3K class II α protein.

PI3 Kinase Class II α (D3Q5B)Rabbit mAb可以识别内源性总的PI3K class II α蛋白。

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly717 of human PI3K class II α protein.

单克隆抗体由合成肽段免疫动物产生,该肽段与人PI3K class II α蛋白的Gly717邻近残基序列一致。

Western Blotting

Western Blotting

Western blot analysis of extracts from SW620 and HeLa cells using PI3 Kinase Class II α (D3Q5B) Rabbit mAb.

使用PI3 Kinase Class II α (D3Q5B)Rabbit mAb兔单抗对SW620和HeLa细胞提取物进行western blot分析。

IP

IP

Immunoprecipitation of PI3 Kinase Class II α from HeLa cell extracts, using Normal Rabbit IgG #2729 (lane 2) or PI3 Kinase Class II α Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using PI3 Kinase Class II α (D3Q5B) Rabbit mAb.

使用正常Rabbit IgG #2729 (lane 2) 或 PI3 Kinase Class II αRabbit mAb 兔单抗(lane 3)对HeLa细胞提取物的PI3 Kinase Class II α进行免疫沉淀实验。Lane1是10%上样量。使用PI3 Kinase Class II α(D3Q5B)Rabbit mAb兔单抗进行western blot分析。

Background

Class II phosphatidylinositol 3-kinases (PI3K) contain a C-terminal C2 domain that is unique to the class II isoforms of the PI3K family. This C2 domain mediates protein and phospholipid binding acitivities (1,2). PI3K Class II α generates phosphatidylinositol 3-phosphate (PIP3) and phosphatidylinositol 3,4-bisphosphate (PI(3, 4)P2) from phosphatidylinositol and phosphatidylinositol 4-phosphate (3). PI3K Class II α is located in various intracellular locations such as the trans-Golgi network, endocytic compartments, clathrin-coated vesicles, and nuclear speckles (1,4,5). Research studies have indicated that PI3K Class II α regulates the assembly and distribution of clathrin, resulting in the modulation of clathrin-dependent trafficking and sorting within the trans Golgi network (5,6). PI3K Class II α also mediates translocation of the glucose transporter GLUT4 to the plasma membrane in response to insulin (7). PI3K Class II α has also been shown to regulate neurosecretory granule exocytosis (8) and vascular smooth muscle contraction (9). Unlike other PI3K family members, PI3K Class II α is less sensitive to the PI3K inhibitors wortmannin and LY294002 (3).

Class II 磷脂酰肌醇3激酶 (PI3K)含有一个C末端C2结构域,使它区别于PI3K家族的其他class II亚型。C2结构域介导了蛋白和磷脂的结合(1,2)。PI3K Class II α 从磷脂酰肌醇(PI)和4-磷酸磷脂酰肌醇(PIP)生成3-磷酸磷脂酰肌醇 (PI(3)P) 和 3,4-二磷酸磷脂酰肌醇 (PI(3, 4)P2)。 PI3K Class II α位于多种细胞内结构,例如转运高尔基体网络,细胞内吞结构,网格蛋白有被小泡和核小(斑)点(1,4,5).研究表明PI3K Class II α 调控网格蛋白的聚集和分布,调控转运高尔基体网络内网格蛋白依赖的示踪和分类(5,6). PI3K Class II α也介导了葡萄糖转运蛋白GLUT4在胰岛素的刺激下转位到细胞膜的过程(7)。PI3K Class II α也被发现可以调控神经分泌颗粒的内吞组员(8)和血管平滑肌收缩(9)。与其他PI3K家族成员不同,PI3K Class II α对于PI3K的抑制剂wortmannin 和 LY294002没有那么敏感(3)。

  1. Didichenko, S.A. and Thelen, M. (2001) J Biol Chem 276, 48135-42.
  2. Stahelin, R.V. et al. (2006) J Biol Chem 281, 39396-406.
  3. Domin, J. et al. (1997) Biochem J 326 ( Pt 1), 139-47.
  4. Domin, J. et al. (2000) J Biol Chem 275, 11943-50.
  5. Gaidarov, I. et al. (2001) Mol Cell 7, 443-9.
  6. Gaidarov, I. et al. (2005) J Biol Chem 280, 40766-72.
  7. Falasca, M. et al. (2007) J Biol Chem 282, 28226-36.
  8. Wen, P.J. et al. (2008) Mol Biol Cell 19, 5593-603.
  9. Yoshioka, K. et al. (2007) Mol Pharmacol 71, 912-20.

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Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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