Cell Signaling Technology

Product Pathways - Autophagy Signaling

BNIP3L/Nix (D4R4B) Rabbit mAb #12396

bnip   BNIP3  

No. Size Price
12396S 100 µl ( 10 western blots ) ¥3,100.00 现货查询 购买询价
12396 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat,Monkey, Endogenous 38, 76 Rabbit IgG
IP 1:100
IF-IC 1:200

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation, IF-IC=Immunofluorescence (Immunocytochemistry),

Specificity / Sensitivity

BNIP3L/Nix (D4R4B) Rabbit mAb recognizes endogenous levels of total BNIP3L/Nix protein.

BNIP3L/Nix (D4R4B) Rabbit mAb 兔单抗能识别内源性的BNIP3L/Nix 总蛋白。

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Glu128 of human BNIP3L/Nix protein.

该单克隆抗体是通过用合成的肽短免疫动物制备的,该合成肽的序列与人BNIP3L/Nix 蛋白的Glu128周围的残基相一致。

Western Blotting

Western Blotting

Western blot analysis of A172 and HeLa cells, untreated (-) or cobalt chloride-treated (100 μM, overnight; +), using BNIP3L/Nix (D4R4B) Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower).

Western blot分析 A172和HeLa 细胞,未处理(-)或chloride(氯化钴)处理(100 μM, 过夜; +),使用的抗体是:BNIP3L/Nix (D4R4B) Rabbit mAb 兔单抗(上图)和 β-Actin (D6A8) Rabbit mAb 兔单抗#8457(下图)。

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using BNIP3L/Nix (D4R4B) Rabbit mAb.

Western blot 分析不同细胞系的提取物,使用的抗体是BNIP3L/Nix (D4R4B) Rabbit mAb 兔单抗。

Western Blotting

Western Blotting

Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with a construct expressing DYKDDDDK-tagged full-length human BNIP3L/Nix (hBNIP3L/Nix-DYKDDDDK; +), using BNIP3L/Nix (D4R4B) Rabbit mAb. The BNIP3L/Nix construct was kindly provided by Dr. Reuben Shaw, Salk Institute for Biological Studies, La Jolla, CA.

Western blot 分析293T细胞的提取物,对照转染(-)或DYKDDDDK标记的全长人BNIP3L/Nix (hBNIP3L/Nix-DYKDDDDK; +)载体转染。使用的抗体是BNIP3L/Nix (D4R4B) Rabbit mAb 兔单抗。BNIP3L/Nix质粒由Dr. Reuben Shaw, Salk Institute for Biological Studies, La Jolla, CA友情提供。

IP

IP

Immunoprecipitation of BNIP3L/Nix from A172 cells, treated with cobalt chloride (100 μM, overnight), using Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) or BNIP3L/Nix (D4R4B) Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using BNIP3L/Nix (D4R4B) Rabbit mAb.

免疫沉淀分析A172细胞中的BNIP3L/Nix ,氯化钴处理 (100 μM, 过夜),使用的抗体是Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (泳道2),BNIP3L/Nix (D4R4B) Rabbit mAb(泳道3)。泳道1是10% input。Western blot 分析用BNIP3L/Nix (D4R4B) Rabbit mAb 兔单抗。

IF-IC

IF-IC

Confocal immunofluorescent analysis of A172 cells, untreated (left) or cobalt chloride-treated (100 μM, 3 hr; right), using BNIP3L/Nix (D4R4B) Rabbit mAb (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

共聚焦免疫荧光分析A172细胞,未处理(左)或氯化钴处理(100 μM, 3 小时; 右),使用的抗体是NIP3L/Nix (D4R4B) Rabbit mAb 兔单抗(绿色)。蓝色伪彩= DRAQ5® #4084(DNA荧光染料)。

Background

BCL2/Adenovirus E1B 19 kDa protein-interacting protein 3-like (BNIP3L) (1), also termed BNIP3α (2), B5 (3), and Nix (4), is a member of the Bcl-2 family of apoptotic regulators with highest homology to BNIP3. BNIP3L can bind BNIP3, Bcl-xL, and Bcl-2 (1-5). BNIP3L forms homodimers that withstand denaturing by SDS and reducing conditions (5). BNIP3L is a mitochondrial protein and knockout studies suggest that BNIP3L regulates autophagic clearance of damaged mitochondria during erythroid maturation via mitochondrial autophagy (6,7). It has been shown that the expression of BNIP3L is up-regulated during terminal erythroid differentiation (6-8), as well as in tumor cell lines during hypoxia (9-11). BNIP3L directly regulates the elimination of mitochondria through its ability to bind to and recruit important components of the autophagic machinery, including LC3/Atg8 and GABARAP proteins, via its amino-terminal LC3-interacting region (LIR) (12). BNIP3L may also indirectly activate phagophore formation either via the recruitment of autophagy proteins or by binding Bcl-xL, which in turn releases Beclin-1 (13). BNIP3L/Nix also plays a pivotal role in Parkin-mediated mitochondrial autophagy via its ability to mediate the mitochondrial translocation of Parkin (14). Activated BNIP3L can promote the opening of mitochondrial permeability transition pores resulting in mitochondrial depolarization, generation of reactive oxygen species, and induction of necrosis. Due to its involvement in cell death and autophagy, research scientists have implicated BNIP3L in heart disease and cancer (13).

BNIP3L(1),也称做BNIP3α(2)、B5(3)或Nix(4),是Bcl-2细胞凋亡调控家族成员之一,与BNIP3有最高的同源性。BNIP3L可以与BNIP3、Bcl-xL和Bcl-2结合(1-5)。BNIP3L形成同源二聚体,能抵抗SDS和还原条件下引起的变性(5)。BNIP3L是一种线粒体蛋白,敲除试验证明,BNIP3L能通过线粒体的自噬,调节红细胞成熟过程中受损线粒体的自噬清除率(6,7)。研究表明,在红细胞最终分化(6-8)和肿瘤细胞系缺氧(9-11)时,BNIP3L的表达出现了上调。BNIP3L能通过其氨基末端的LC3相互作用区,结合并补充自噬机制的重要组成部分,从而来直接调节线粒体的清除,该组成部分包括LC3/Atg8和GABARAP蛋白(12)。BNIP3L也能通过补充自噬蛋白或结合能依次释放Becclin-1的Bcl-xL来间接激活吞噬泡的形成(13)。由于BNIP3L/Nix能介导线粒体的二乙嗪迁移,其在二乙嗪介导的线粒体自噬中也有举足轻重的作用(14)。活化的BNIP3L能够促进线粒体通透性转换孔的开启,从而引起线粒体的去极化、活性氧的产生和坏死的诱导。由于它参与细胞死亡和自噬,研究者在心脏疾病和癌症的研究中也涉及到BNIP3L(13)。

  1. Matsushima, M. et al. (1998) Genes Chromosomes Cancer 21, 230-5.
  2. Yasuda, M. et al. (1999) Cancer Res 59, 533-7.
  3. Ohi, N. et al. (1999) Cell Death Differ 6, 314-25.
  4. Chen, G. et al. (1999) J Biol Chem 274, 7-10.
  5. Imazu, T. et al. (1999) Oncogene 18, 4523-9.
  6. Schweers, R.L. et al. (2007) Proc Natl Acad Sci USA 104, 19500-5.
  7. Sandoval, H. et al. (2008) Nature 454, 232-5.
  8. Aerbajinai, W. et al. (2003) Blood 102, 712-7.
  9. Sowter, H.M. et al. (2001) Cancer Res 61, 6669-73.
  10. Bruick, R.K. (2000) Proc Natl Acad Sci USA 97, 9082-7.
  11. Fei, P. et al. (2004) Cancer Cell 6, 597-609.
  12. Novak, I. et al. (2010) EMBO Rep 11, 45-51.
  13. Zhang, J. and Ney, P.A. (2009) Cell Death Differ 16, 939-46.
  14. Ding, W.X. et al. (2010) J Biol Chem 285, 27879-90.

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