Cell Signaling Technology

Product Pathways - Metabolism

DLAT (4A4-B6-C10) Mouse mAb #12362

70 kDa mitochondrial autoantigen of primary biliary cirrhosis   Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex   dihydrolipoamide S-acetyltransferase   Dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex. mitochondrial   DLAT   DLTA   E2 component of pyruvate dehydrogenase complex   M2 antigen complex 70 kDa subunit   ODP2   PBC   PDC-E2   PDCE2   Pyruvate dehydrogenase complex component E2  

No. Size Price
12362S 100 µl ( 10 western blots ) ¥3,100.00 现货查询 购买询价
12362 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Monkey, Endogenous 69 Mouse IgG1
IP 1:50
IF-IC 1:100

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation, IF-IC=Immunofluorescence (Immunocytochemistry),

Specificity / Sensitivity

DLAT (4A4-B6-C10) Mouse mAb recognizes endogenous levels of total DLAT protein.

DLAT (4A4-B6-C10) Mouse mAb 能够检测内源性DLAT总蛋白水平。

Source / Purification

Monoclonal antibody is produced by immunizing animals with a recombinant human DLAT protein.

该单克隆抗体是由重组人源DLAT蛋白亮疫动物而制备的。

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using DLAT (4A4-B6-C10) Mouse mAb.

Western blot检测多种细胞提取物,使用抗体为DLAT (4A4-B6-C10) Mouse mAb鼠单抗。

IP

IP

Immunoprecipitation of DLAT from HeLa cell extracts using Mouse (G3A1) mAb IgG1 Isotype Control #5415 (lane 2) or DLAT (4A4-B6-C10) Mouse mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using DLAT (4A4-B6-C10) Mouse mAb.

免疫沉淀分析HeLa细胞提取物DLAT, 使用Mouse (G3A1) mAb IgG1 Isotype Control #5415 (泳道 2) 或 DLAT (4A4-B6-C10) Mouse mAb 鼠单抗(泳道3)。泳道1是10%对照。使用 DLAT (4A4-B6-C10) Mouse mAb鼠单抗进行Western blot检测。

IF-IC

IF-IC

Confocal immunofluorescent analysis of HeLa cells using DLAT (4A4-B6-C10) Mouse mAb (green). Actin filaments were labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

共聚焦免疫荧光分析HeLa 细胞,使用抗体为DLAT (4A4-B6-C10) Mouse mAb 鼠单抗(绿色) ,DY-554 phalloidin (红色) 标记肌动蛋白纤丝。 蓝色伪彩=DRAQ5®#4084(荧光DNA染料)。

Background

Dihydrolipoamide acetyltransferase (DLAT) transfers an acetyl group from pyruvate to CoA to synthesize acetyl-CoA (1-4). This protein, also known as the E2 subunit of the pyruvate dehydrogenase complex (PDC-E2), has been implicated in the literature as the primary autoantigen in primary biliary cirrhosis (2-5). Antimitochondrial antibodies (AMAs) are likely formed when DLAT is exposed to the immune system in apoptotic cells of the bile duct (3,5). Research studies have shown that in some cases, cosmetics, NSAIDs, chewing gum, acetaminophen, and other compounds could trigger exposure of DLAT in sensitive individuals (3). The presence of AMAs is often detectable before disease diagnosis (4,5). Research studies have also shown that activation of the Toll-like receptor-3 (TLR-3) pathway is involved in the progression from a subclinical to clinical state (4).

二氢硫辛酰胺乙酰转移酶(DLAT)催化一个乙酰基从丙酮酸转移到辅酶A,进而合成乙酰辅酶A(1-4)。已知这种蛋白质是丙酮酸脱氢酶复合物E2亚单位(PDC-E2),文献提到其在原发性胆汁性肝硬化中是主要的自身抗原(2-5)。当DLAT暴露在胆管凋亡细胞的免疫系统中时,有可能形成抗线粒体抗体(AMAS)(3,5)。研究表明,在某些情况下,化妆品,非甾体抗炎药,口香糖,对乙酰氨基酚和其它化合物可能会引发敏感个体DLAT的暴露(3)。 AMAS的存在疾病诊断前往往被检测到(4,5)。研究还表明,激活的Toll样受体(TLR-3)通路参与从亚临床状态到临床状态的变化(4)。

  1. Thekkumkara, T.J. et al. (1988) FEBS Lett 240, 45-8.
  2. Coppel, R.L. et al. (1988) Proc Natl Acad Sci U S A 85, 7317-21.
  3. Naiyanetr, P. et al. (2011) J Autoimmun 37, 209-16.
  4. Ambrosini, Y.M. et al. (2011) Clin Exp Immunol 166, 110-20.
  5. Lleo, A. et al. (2009) Hepatology 49, 871-9.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

DRAQ5 is a registered trademark of Biostatus Limited.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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