Cell Signaling Technology

Product Pathways - Chromatin Regulation / Epigenetics

ARID1A/BAF250A (D2A8U) Rabbit mAb #12354

No. Size Price
12354S 100 µl ( 10 western blots ) ¥3,100.00 现货查询 购买询价
12354 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat,Monkey, Endogenous 250 Rabbit IgG
IHC-P 1:500

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IHC-P=Immunohistochemistry (Paraffin),

Specificity / Sensitivity

ARID1A/BAF250A (D2A8U) Rabbit mAb recognizes endogenous levels of total ARID1A/BAF250A protein. This antibody also cross-reacts with proteins of unknown origin at 65 kDa.

ARID1A/BAF250A(D2A8U) Rabbit mAb兔单抗能够检测内源性的ARID1A/BAF250A总蛋白水平。该抗体还能与未知来源的分子量为65kD的蛋白发生交叉反应。

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly1293 of human ARID1A/BAF250A protein.


Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using ARID1A/BAF250A (D2A8U) Rabbit mAb.

Western blot检测各种细胞系提取物,使用ARID1A/BAF250A(D2A8U) Rabbit m Ab鼠单抗。

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded cell pellets, COS-7 (left) or T-47D (right), using ARID1A/BAF250A (D2A8U) Rabbit mAb.

石蜡包埋细胞的免疫组化,cos-7(左)或T-47D(右),使用抗体为ARID1A/BAF250A(D2A8U) Rabbit mAb兔单抗.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human lung adenosquamous carcinoma using ARID1A/BAF250A (D2A8U) Rabbit mAb in the presence of control peptide (left) or antigen-specific peptide (right).

人肺腺癌的免疫组化石蜡切片,使用抗体为ARID1A/BAF250A(D2A8U) Rabbit mAb兔单抗,在对照肽段中有表达(图片左),右图为抗原特异肽段。

Western Blotting

Western Blotting

Western blot analysis of T-47D and Jurkat cell extracts using ARID1A/BAF250A (D2A8U) Rabbit mAb (upper) or ß-Actin (D6A8) Rabbit mAb #8457 (lower). Additional ARID1A/BAF250A degradation products may be detected in some cell extracts between 135kDa-250kDa, which are absent in the ARID1A/BAF250A negative T-47D cell line.

Western blot方法检测T-47D和Jurkat细胞系的提取物,使用的抗体为ARID1A/BAF250A(D2A8U) rabbit mAb兔单抗(上面)或者β-actin(D6A8) rabbit mAb#8457兔单抗(下面)。一些ARID1A/BAF250A降解的产物可能在一些细胞提取物中检测到,分子量位于135kDa到250kDa之间,但是在T-47D细胞系中检测不到。

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded cell pellets, COS-7 (left) or T-47D (right), using ARID1A/BAF250A (D2A8U) Rabbit mAb.

石蜡包埋细胞的免疫组化,cos-7(左)或T-47D(右),使用抗体为ARID1A/BAF250A(D2A8U) Rabbit mAb兔单抗。


ATP-dependent chromatin remodeling complexes play an essential role in the regulation of nuclear processes such as transcription and DNA replication and repair (1,2). The SWI/SNF chromatin remodeling complex consists of more than 10 subunits and contains a single molecule of either BRM or BRG1 as the ATPase catalytic subunit. The activity of the ATPase subunit disrupts histone-DNA contacts and changes the accessibility of crucial regulatory elements to the chromatin. The additional core and accessory subunits play a scaffolding role to maintain stability and provide surfaces for interaction with various transcription factors and chromatin (2-5). The interactions between SWI/SNF subunits and transcription factors, such as nuclear receptors, p53, Rb, BRCA1, and MyoD, facilitate recruitment of the complex to target genes for regulation of gene activation, cell growth, cell cycle, and differentiation processes (1,6-9).

ATP依赖的染色质重塑复合物,在调节如转录、DNA的复制和修复等细胞核反应起着重要作用(1,2)。SWI/SNF染色质重塑复合物由10多个亚基组成,包含BRM或BRG1任一单分子作为ATP酶催化亚基。ATP酶亚基活性能够破坏组蛋白与DNA的结合,同时改变染色质重要调控元件与染色质的结合。额外的核心和配体亚基起着支架作用,有助于稳定各种转录因子和染色质的相互作用,为其提供作用表面(2-5)。SWI/SNF亚基与各种转录因子如核受体,p53, Rb, BRCA1和MyoD等的相互作用,有利于招募复合物结合目标基因,对基因的激活、细胞生长、细胞周期和分化过程进行调控(1,6-9)。

ARID1A is one of the accessory subunits of the SWI/SNF complex that is an essential part of the esBAF (mouse embryonic stem cell specific SWI/SNF complex). ARID1A is critical for ES cell pluripotency and differentiation into mesoderm-derived cardiomyocytes and adipocytes (10). In addition, ARID1A has been found to be frequently mutated in several cancers such as uterine and ovarian endometrioid carcinoma and ovarian clear cell carcinoma (11-13).


  1. Ho, L. and Crabtree, G.R. (2010) Nature 463, 474-84.
  2. Becker, P.B. and Hörz, W. (2002) Annu Rev Biochem 71, 247-73.
  3. Eberharter, A. and Becker, P.B. (2004) J Cell Sci 117, 3707-11.
  4. Bowman, G.D. (2010) Curr Opin Struct Biol 20, 73-81.
  5. Gangaraju, V.K. and Bartholomew, B. (2007) Mutat Res 618, 3-17.
  6. Lessard, J.A. and Crabtree, G.R. (2010) Annu Rev Cell Dev Biol 26, 503-32.
  7. Morettini, S. et al. (2008) Front Biosci 13, 5522-32.
  8. Wolf, I.M. et al. (2008) J Cell Biochem 104, 1580-6.
  9. Simone, C. (2006) J Cell Physiol 207, 309-14.
  10. Guan, B. et al. (2011) Am J Surg Pathol 35, 625-32.
  11. Gao, X. et al. (2008) Proc Natl Acad Sci U S A 105, 6656-61.
  12. Jones, S. et al. (2010) Science 330, 228-31.
  13. Wiegand, K.C. et al. (2010) N Engl J Med 363, 1532-43.

Application References

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!


Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

用户评论 --- 共 0


我要参与评论 :