Cell Signaling Technology

Product Pathways - Cytoskeletal Signaling

α-Tubulin (DM1A) Mouse mAb (HRP Conjugate) #12351

sc-5546  

No. Size Price
12351S 100 µl ( 10 western blots ) ¥3,986.00 现货查询 购买询价
12351 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat,Monkey, Endogenous 52 Mouse IgG1

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting,

Specificity / Sensitivity

α-Tubulin (DM1A) Mouse mAb (HRP Conjugate) recognizes endogenous levels of total α-tubulin protein.

α-Tubulin (DM1A)Mouse mAb(偶联HRP)可以识别内源性总的α-tubulin蛋白。

Source / Purification

Monoclonal antibody is produced by immunizing animals with full-length chicken α-tubulin purified from brain extracts, and recognizes residues surrounding Val440 of α-tubulin protein.

合成对应来自脑提取物的全长鸡α-tubulin可以识别其Val440及其邻近残基序列的多肽免疫动物获得单抗。

Description

This Cell Signaling Technology antibody is conjugated to the carbohydrate groups of horseradish peroxidase (HRP) via its amine groups. The HRP conjugated antibody is expected to exhibit the same species cross-reactivity as the unconjugated α-Tubulin (DM1A) Mouse mAb #3873.Cell Signaling Technology抗体与辣根过氧化物酶(HRP)的碳水化合物基团偶联。HRP偶联的抗体被认为与未偶联的α-Tubulin (DM1A)Mouse mAb #3873具有相同的交叉反应性。

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using α-Tubulin (DM1A) Mouse mAb (HRP Conjugate).使用α-Tubulin (DM1A)Mouse mAb(偶联HRP)对多种细胞提取物进行western blot分析。

Background

The cytoskeleton consists of three types of cytosolic fibers: microtubules, microfilaments (actin filaments), and intermediate filaments. Globular tubulin subunits comprise the microtubule building block, with α/β-tubulin heterodimers forming the tubulin subunit common to all eukaryotic cells. γ-tubulin is required to nucleate polymerization of tubulin subunits to form microtubule polymers. Many cell movements are mediated by microtubule action, including the beating of cilia and flagella, cytoplasmic transport of membrane vesicles, chromosome alignment during meiosis/mitosis, and nerve-cell axon migration. These movements result from competitive microtubule polymerization and depolymerization or through the actions of microtubule motor proteins (1).

细胞骨架包括三种细胞质纤维:微管,微丝(肌动蛋白丝)和中间丝。球形微管亚基由microtubule building block组成,在所有的真核细胞中,α/β-tubulin异二聚体形成的微管亚基是很常见的。γ-tubulin对于tubulin亚基的细胞核多聚体话以形成微管复合物是必须的。许多细胞运动都是通过微管的活动介导的,包括纤毛和鞭毛的跳动,胞质膜小泡的运输、减数分裂/有丝分裂时的染色质对齐,和神经细胞轴突迁移。这些运动是微管聚合和结局的竞争结果,或者是通过微管马达蛋白的作用而发生的(1)。

  1. Westermann, S. and Weber, K. (2003) Nat Rev Mol Cell Biol 4, 938-47.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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