Cell Signaling Technology

Product Pathways - DNA Damage

ERCC1 (D6G6) XP® Rabbit mAb #12345

ERCC-1   ERCC1  

No. Size Price
12345S 100 µl ( 10 western blots ) ¥3,580.00 现货查询 购买询价
12345T 20 µl ( 2 western blots ) ¥1,400.00 现货查询 购买询价
12345 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat,Monkey, Endogenous 39 Rabbit IgG
IHC-P 1:125

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IHC-P=Immunohistochemistry (Paraffin),

Specificity / Sensitivity

ERCC1 (D6G6) XP® Rabbit mAb recognizes endogenous levels of total ERCC1 protein. This antibody also detects ERCC1 splice variants around 30 kDa.ERCC1 (D6G6) XP®Rabbit mAb可以识别内源性总的ERCC1蛋白。抗体可以检测30kDa左右的基因剪接突变体。

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human ERCC1 protein.单克隆抗体由合成肽段免疫动物产生,该肽段与人ERCC1蛋白的羧基端残基序列一致。

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded cell pellets, HeLa (left) or XP2Y0 (right), using ERCC1 (D6G6) XP® Rabbit mAb.使用ERCC1 (D6G6) XP®Rabbit mAb对石蜡包埋的细胞进行免疫组化分析,HeLa (左) 或 XP2Y0 (右)。

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human breast carcinoma using ERCC1 (D6G6) XP® Rabbit mAb. 使用ERCC1 (D6G6) XP®Rabbit mAb对石蜡包埋的人乳腺癌组织进行免疫组化分析。

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human lung carcinoma using ERCC1 (D6G6) XP® Rabbit mAb. 使用ERCC1 (D6G6) XP®Rabbit mAb对石蜡包埋的人肺癌组织进行免疫组化分析。

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using ERCC1 (D6G6) XP® Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower).使用ERCC1 (D6G6) XP®Rabbit mAb(上)或β-Actin (D6A8) Rabbit mAb #8457(下)对多种细胞提取物进行western blot分析。

Background

DNA repair systems operate in all living cells to manage a variety of DNA lesions. Nucleotide excision repair (NER) is implemented in cases where bulky helix-distorting lesions occur, such as those brought about by UV and certain chemicals (1). Excision Repair Cross Complementing 1 (ERCC1) forms a complex with XPF, which acts as the 5’ endonuclease required to excise the lesion (2). ERCC1-XPF is also required for repair of DNA interstrand crosslinks (ICLs) (3) and involved in repair of double strand breaks (4). Research studies have shown that expression of ERCC1 is related to survival rate and response to chemotherapeutic drugs in several human cancers including non-small cell lung cancer (NSCLC) (5,6).所有活细胞都有DNA损伤修复功能以调控DNA损伤。核苷酸切除修复出现在大片段的螺旋-扭曲损伤时,例如由UV或某种化学物质引发的损伤(1)。Excision Repair Cross Complementing 1 (ERCC1)与XPF构成复合物,作为5’内切酶切除掉发生损伤的部位(2)。ERCC1-XPF还在DNA链间交联(ICLs)(3)和双链损伤时(4)发挥足以。研究证明ERCC1的表达与存活率相关,也在一些人类癌症疾病,包括肺小细胞癌(NSCLC)对化疗的的反应过程相关(5,6)。

  1. Shuck, S.C. et al. (2008) Cell Res 18, 64-72.
  2. McDaniel, L.D. and Schultz, R.A. (2008) Adv Exp Med Biol 637, 65-82.
  3. Niedernhofer, L.J. et al. (2004) Mol Cell Biol 24, 5776-87.
  4. Ahmad, A. et al. (2008) Mol Cell Biol 28, 5082-92.
  5. Zheng, Z. et al. (2007) N Engl J Med 356, 800-8.
  6. Gossage, L. and Madhusudan, S. (2007) Cancer Treat Rev 33, 565-77.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

XP is a registered trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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