Cell Signaling Technology

Product Pathways - Growth Factors/Cytokines

Human His6Interleukin-25/IL-17E (hHis6IL-25) #12337

No. Size Price
12337LC 50 µg ( With Carrier ) ¥6,860.00 现货查询 购买询价
12337LF 50 µg ( Carrier Free ) ¥6,860.00 现货查询 购买询价
12337SC 10 µg ( With Carrier ) ¥2,321.00 现货查询 购买询价
12337SF 10 µg ( Carrier Free ) ¥2,321.00 现货查询 购买询价
12337 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype

Species cross-reactivity is determined by western blot.

Applications Key:

Source / Purification

Recombinant human His6IL-25 (hHis6IL-25) Tyr33-Gly177 (Accession #NP_073626) was expressed in human 293 cells at Cell Signaling Technology.

重组人His6IL-25 (hHis6IL-25) Tyr33-Gly177 (Accession #NP_073626) 通过Cell Signaling Technology公司的人源293细胞表达。

Molecular Characterization

Recombinant N-terminally His6-tagged hIL-25 has a calculated MW of 19,318. DTT-reduced protein migrates as a 26-32 kDa polypeptide. The nonreduced cystine-linked homodimer migrates as a 40-46 kDa protein. Lower mobility and heterogeneity in SDS-PAGE are due to glycosylation. The expected amino terminus of recombinant hHis6IL-25 was verified by amino acid sequencing.

重组的hIL-25蛋白在N-末端有His6标签,其分子量据推算为19,318 Da。DTT还原蛋白迁移率接近26-32kDa 的多肽。未还原的胱氨酸相连的同源二聚体迁移蛋白大约40-46 kDa。其在SDS-PAGE胶中的低迁移性和多态性与糖基化有关。重组hHis6IL-6蛋白的N-末端序列通过测序得到。

Purity

>97% as determined by SDS-PAGE of 6 μg reduced (+) and nonreduced (-) recombinant hHis6IL-25. 30% migrates as monomer under nonreducing conditions. All lots are greater than 97% pure.

通过SDS-PAGE确定还原型(+)和未还原型(-)的重组hHis6IL-25的纯度大于97%。在未还原的环境下,30%会作为单体发生迁移。所有样品的纯度均高于97%。

Bioactivity

The bioactivity of recombinant hHis6IL-25 was determined by its ability to induce IL-5 production from TSLP-primed PBMC in the presence of IL-2. The ED50 of each lot is between 5-15 pg/ml.

重组人His6IL-25的生物活性通过对存在IL-2的TSLP预处理的PBMC 诱导产生IL-5的能力来确定。每个样品的ED50范围在5-15 pg/ml。

Coomassie Gel

Coomassie Gel

The purity of recombinant hHis6IL-25 was determined by SDS-PAGE of 6 µg reduced (+) and nonreduced (-) recombinant hHis6IL-25 and staining overnight with Coomassie Blue.通过SDS-PAGE纯化重组hHis6IL-25,方法是加入6 µg 诱导 (+)和未诱导的 (-) 重组hHis6IL-25,考马斯亮蓝染色过夜。

Bioactivity

Bioactivity

IL-5 production from human PBMC costimulated with IL-2 and increasing concentrations of hHis6IL-25 was assessed. PBMC were treated with TSLP (100 ng/ml, 24 hr) and then costimulated with IL-2 (10 ng/ml) and increasing concentrations of hHis6IL-25. After 72 hr, cell supernatants were harvested and assayed for IL-5 by ELISA and the OD450 was determined.IL-5是人源PBMC细胞中通过使用IL-2与浓度递增的hHis6IL-25共刺激后产生的。PBMC细胞使用TSLP (100 ng/ml, 24 小时)处理,接着使用IL-2 (10 ng/ml)和浓度递增的hHis6IL-25共刺激。72小时后,收集细胞上清,并且通过ELISA和OD450分析IL-5。

Endotoxin

Less than 0.01 ng endotoxin/1 μg hHis6IL-25.

每微克hHis6IL-25内毒素含量少于0.01 ng。

Formulation

With carrier: Lyophilized from a 0.22 μm filtered solution of hHis6IL-25 in 20 mM Tris, pH 7.2 containing 150 mM NaCl and 20 μg BSA per 1 μg hHis6IL-25. Carrier free: Lyophilized from a 0.22 μm filtered solution of hHis6IL-25 in 20 mM Tris, pH 7.2 containing 150 mM NaCl.

有载体:每微克hHis6IL-25溶解在包含 20 μg BSA和150 mM NaCl的20 mM Tris (pH 7.2)溶液中,并通过 0.22 μm 滤膜过滤后冻干。

无载体:每微克hHis6IL-25溶解在包含 150 mM NaCl的20 mM Tris (pH 7.2)溶液中,并通过 0.22 μm 滤膜过滤后冻干。

Background

IL-25, also known as IL-17E, is a member of the IL-17 superfamily of cytokines. IL-25 is expressed in epithelial cells, CD4+ T cells, mast cells, and eosinophils (1). Many cell types are responsive to IL-25, including T cells, macrophages, and epithelial cells (1). The receptor for IL-25 consists of a heterodimer of IL-17RA and IL-17RB (1,2). IL-25 promotes Th2 type immune responses by induction of IL-5, IL-4, and IL-13 and may contribute to allergic inflammation and asthma (1-3). IL-25 has also been shown in research to promote Th9 cell activation and induces apoptosis in breast cancer cells (4,5).

IL-25,又称IL-17E,是IL-17超家族细胞因子成员之一。IL-25在上皮细胞,CD4+T细胞,肥大细胞以及嗜酸性粒细胞中表达(1)。多种细胞类型都可以对IL-25产生应答,如T细胞,巨噬细胞和上皮细胞(1)。IL-25受体是由IL-17RA和IL-17RB组成的异源二聚体(1,2)。IL-25能够通过诱导IL-5、IL-4和IL-13促进Th2型免疫应答,同时可能有助于过敏性炎症和哮喘的发生(1-3)。IL-25同样能够促进Th9细胞的活化以及诱导乳腺癌细胞的凋亡(4,5)。

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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