Cell Signaling Technology

Product Pathways - Protein Translation

Pumilio 2 Antibody #12323

No. Size Price
12323S 100 µl ( 10 western blots ) ¥3,100.00 现货查询 购买询价
12323 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse, Endogenous 110-125 Rabbit
IP 1:100

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation,

Homology

Species predicted to react based on 100% sequence homology: Rat, Monkey,

Specificity / Sensitivity

Pumilio 2 Antibody recognizes endogenous levels of total Pumilio 2 protein. Pumilio 2 Antibody is expected to recognize all three known Pumilio 2 protein isoforms. Cross reactive bands of unknown origin are observed around 45 kDa.

Pumilio 2 Antibody能够检测内源性的 Pumilio 2总蛋白水平。 Pumilio 2 Antibody预计能够识别所有三种已知的 Pumilio 2异构体。该抗体还能够与一个未知来源的45kDa蛋白发生交叉反应。

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human Pumilio 2 protein. Antibodies are purified by protein A and peptide affinity chromatography.

该多克隆抗体是经由合成的围绕人Pumilio 2蛋白近羧基端的氨基酸肽段免疫动物而生产的。经过Protein A和亲和层析纯化。

Western Blotting

Western Blotting

Western blot analysis of extracts from A172 and NTERA-2 cl.D1 cells using Pumilio 2 Antibody.

Western blot方法检测A172 和 NTERA-2 cl.D1细胞的提取物,使用的抗体为Pumilio 2 Antibody。

Background

Pumilio 1 and Pumilio 2 (PUM1, PUM2, or Pumilio homolog 1 and 2, respectively) are evolutionarily conserved RNA binding proteins that are thought to repress translation and stability of mRNA targets by binding to the 3'-UTR of specific RNA sequences (1). Pumilio proteins have been implicated in the regulation of genes involved in embryogenesis and germline cell development (2). Research studies have shown that PUM2 may have a role in neural stem cell fate decisions (3).

Pumilio 1 和Pumilio 2 (PUM1, PUM2, 或 Pumilio 同源物1 和 2)是进化上保守的RNA结合蛋白,研究认为它们能够通过与特定RNA序列的3'-UTR结合来抑制翻译和mRNA靶标的稳定性(1)。Pumilio蛋白涉及了参与胚胎形成和生殖细胞发育的基因的调控(2)。研究表明PUM2可能在神经干细胞命运决定中发挥作用(3)。

  1. Weidmann, C.A. and Goldstrohm, A.C. (2012) Mol Cell Biol 32, 527-40.
  2. Spassov, D.S. and Jurecic, R. (2003) IUBMB Life 55, 359-66.
  3. Vessey, J.P. et al. (2012) Cell Stem Cell , .

Application References

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Protocols

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For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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