Cell Signaling Technology

Product Pathways - Protein Translation

PKR (D7F7) Rabbit mAb #12297

double stranded RNA activated protein kinase   E2AK2   eIF-2A protein kinase 2   EIF2AK1   EIF2AK2   Eukaryotic translation initiation factor 2-alpha kinase 2   Interferon-induced. double-stranded RNA-activated protein kinase   interferon-inducible elF2alpha kinase   Interferon-inducible RNA-dependent protein kinase   MGC126524   P1/eIF-2A protein kinase   p68 kinase   PKR   PRKR   Protein kinase RNA-activated   protein kinase. interferon-inducible double stranded RNA dependent   sc-6282  

No. Size Price
12297S 100 µl ( 10 western blots ) ¥3,100.00 现货查询 购买询价
12297 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human, Endogenous 74 Rabbit IgG
IP 1:50

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation,

Specificity / Sensitivity

PKR (D7F7) Rabbit mAb recognizes endogenous levels of total PKR protein.

PKR (D7F7) Rabbit mAb能够检测内源性的 PKR总蛋白。

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu222 of human PKR protein.

该单克隆抗体经由合成的围绕人 PKR蛋白 Leu222位点的氨基酸肽段免疫动物而生产的。

Western Blotting

Western Blotting

Western blot analysis of extracts from PANC-1, RD, and HeLa cells using PKR (D7F7) Rabbit mAb.

Western blot方法检测PANC-1、 RD和HeLa细胞提取物,使用的抗体为PKR (D7F7) Rabbit mAb兔单抗。



Immunoprecipitation of PKR from PANC-1 cell extracts, using Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) or PKR (D7F7) Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using PKR (D7F7) Rabbit mAb.

免疫沉淀法检测PANC-1细胞提取物中的PKR,使用抗体为Rabbit (DA1E) mAb IgG XP® Isotype Control #3900(泳道2)或PKR (D7F7) Rabbit mAb兔单抗 (泳道3)。泳道1为10% input对照。 Western blot 检测中使用的是PKR (D7F7) Rabbit mAb兔单抗。


Protein kinase R (PKR) is transcriptionally induced by interferon and activated by double-stranded RNA (dsRNA). PKR inhibits translation initiation through phosphorylation of the α subunit of the initiation factor eIF2 (eIF2α) and also controls the activation of several transcription factors, such as NF-κB, p53, and the Stats. In addition, PKR mediates apoptosis induced by many different stimuli, such as LPS, TNF-α, viral infection, and serum starvation (1,2). Activation of PKR by dsRNA results in PKR dimerization and autophosphorylation of Thr446 and Thr451 in the activation loop. Substitution of threonine for alanine at position 451 completely inactivated PKR, while a mutant with a threonine to alanine substitution at position 446 was partially active (3). Research studies have implicated PKR activation in the pathologies of neurodegenerative diseases, including Alzheimer's disease (4,5).

Protein kinase R (PKR)是通过干扰素诱导转录并被双链RNA活化(dsRNA)。PKR可以通过磷酸化起始因子 eIF2的α亚基(eIF2α),以及控制多种转录因子的激活如NF-κB、p53以及Stats,从而抑制翻译的起始的。另外,PKR可介导由许多不同刺激导致的细胞凋亡,如LPS、TNF-α、病毒感染以及血清饥饿 (1,2)。由dsRNA活化的PKR会导致PKR的二聚体化和活化环内Thr446和Thr451位点的自身磷酸化。当451位点的苏氨酸被丙氨酸替代时会是PKR完全失活,而446位点的苏氨酸突变为丙氨酸则只导致部分失活(3)。研究显示在神经退行性疾病包括阿兹海默氏症的病理中都出现PKR的活化(4,5)。

  1. Williams, B.R. (1999) Oncogene 18, 6112-6120.
  2. Gil, J. and Esteban, M. (2000) Apoptosis 5, 107-114.
  3. Romano, P. R. et al. (1998) Mol. Cell. Biol. 18, 2282-2297.
  4. Peel, A.L. and Bredesen, D.E. (2003) Neurobiol. Dis. 14, 52-62.
  5. Peel, A.L. (2004) J. Neuropath. Exp. Neurol. 63, 97-105.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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