Cell Signaling Technology

Product Pathways - Growth Factors/Cytokines

Human Fibroblast Growth Factor 9 (hFGF9) #12296

No. Size Price
12296LC 50 µg ( With Carrier ) ¥5,949.00 现货查询 购买询价
12296LF 50 µg ( Carrier Free ) ¥5,949.00 现货查询 购买询价
12296SC 10 µg ( With Carrier ) ¥2,061.00 现货查询 购买询价
12296SF 10 µg ( Carrier Free ) ¥2,061.00 现货查询 购买询价
12296 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype

Species cross-reactivity is determined by western blot.

Applications Key:

Source / Purification

Recombinant human FGF9 (hFGF9) Leu4-Ser208 (Accession #NP_31371) was expressed in E.coli at Cell Signaling Technology.

Cell Signaling Technology使用E.coli表达重组人FGF9 (hFGF9) Leu4-Ser208 蛋白(Accession #NP_31371)。

Molecular Characterization

Recombinant hFGF9 has a calculated MW of 23,141 Da. DTT-reduced and nonreduced protein migrates as a 23 kDa polypeptide. The expected amino terminus of recombinant hFGF9 was verified by amino acid sequencing.

重组hFGF9分子量为23,141 Da.DTT-还原的和未还原的蛋白迁移率为23 kDa多肽。重组hGFG9的氨基端序列由氨基酸测序确认。


>95% as determined by SDS-PAGE of 6 μg reduced (+) and nonreduced (-) recombinant hFGF9. All lots are greater than 95% pure.

通过SDS-PAGE确认6 μg还原(+)和未还原(-)重组hFGF9纯度大于>95%。所有的批次纯度都大于>95%。


The bioactivity of hFGF9 was determined in an NIH/3T3 cell proliferation assay. The ED50 of each lot is between 0.05-0.80 ng/ml.

使用NIH/3T3细胞扩增assay检测hFGF9的生物活性。每个批次的ED50都在0.05-0.80 ng/ml之间。



The proliferation of NIH/3T3 cells treated with increasing concentrations of hFGF9 was assessed. After 24 hr treatment, cells were labeled with BrdU for 4 hrs. BrdU incorporation was determined by ELISA and the OD450 was determined.


Coomassie Gel

Coomassie Gel

The purity of recombinant hFGF9 was determined by SDS-PAGE of 6 µg reduced (+) and nonreduced (-) recombinant hFGF9 and staining overnight with Coomassie Blue.

通过SDS-PAGE确认6 μg还原(+)和未还原(-)重组hFGF9的纯度,并使用考马斯亮蓝染色过夜。


Less than 0.01 ng endotoxin/1 μg hFGF9.

每1μg hFGF9含有内毒素低于0.01 ng.


With carrier: Lyophilized from a 0.22 μm filtered solution of hFGF9 in 20 mM Tris, pH 7.2 containing 20 μg BSA per 1 μg hFGF9. Carrier free: Lyophilized from a 0.22 μm filtered solution of hFGF9 in 20 mM Tris, pH 7.2.

带载体:hFGF9 in 20 mM Tris, pH 7.2 containing 20 μg BSA per 1 μg hFGF9经过0.22 μm过滤后冻干。无载体:hFGF9 in 20 mM Tris, pH 7.2经过0.22 μm过滤后冻干。


Fibroblast growth factor (FGF) 9 is a member of the larger FGF family of proteins that play key roles in development, cancer, and metabolism. Binding of FGF9 to its receptor requires interaction with heparin and induces receptor dimerization, subsequent transphosphorylation, and downstream activation of Erk, Akt, and PLCγ pathways (1). FGF9 is important for organ development and bone repair (2-4).

成纤维细胞生长因子(FGF)9是FGF家族蛋白一员,在发育,癌症和代谢过程中发挥关键作用。FGF9与受体结合需要肝素的作用诱导受体二聚化,随后转磷酸化,下游激活Erk, Akt, 和 PLCγ信号通路(1). FGF9在器官发育和骨骼修复时有重要作用(2-4)。

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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