Cell Signaling Technology

Product Pathways - Protein Translation

FXR1 (D10A2) XP® Rabbit mAb #12295

Fragile X mental retardation syndrome-related protein 1   fragile X mental retardation. autosomal homolog 1   FXR   FXR1   hFXR1p  

No. Size Price
12295S 100 µl ( 10 western blots ) ¥3,750.00 现货查询 购买询价 防伪查询
12295T 20 µl ( 2 western blots ) ¥1,400.00 现货查询 购买询价 防伪查询
12295 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat,Monkey, Endogenous 78-80, 82-84 Rabbit IgG
IF-IC 1:50

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IF-IC=Immunofluorescence (Immunocytochemistry),

Specificity / Sensitivity

FXR1 (D10A2) XP® Rabbit mAb recognizes endogenous levels of total FXR1 protein.

FXR1 (D10A2) XP® Rabbit mAb能够检测内源性 FXR1总蛋白。

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly574 of human FXR1 protein.

该单克隆抗体是经由合成的围绕人 FXR1蛋白 Gly574位点的氨基酸肽段免疫动物而生产的。



Confocal immunofluorescent analysis of C2C12 cells, untreated (left) or MG-132 treated (10 μg/mL, 3 hr; right), using FXR1 (D10A2) XP® Rabbit mAb (green). Actin filaments were labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

采用共聚焦免疫荧光术分别检测未处理(左)或MG-132 处理 (10 μg/mL, 3小时; 右)的 C2C12细胞,使用的抗体为 FXR1 (D10A2) XP® Rabbit mAb兔单抗 (绿色)。肌动蛋白微丝使用DY-554 phalloidin进行标记(红色)。蓝色伪彩= DRAQ5® #4084 (荧光DNA染料)。

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using FXR1 (D10A2) XP® Rabbit mAb.

Western blot方法检测多种细胞系的提取物,使用的抗体为 FXR1 (D10A2) XP® Rabbit mAb兔单抗。

Western Blotting

Western Blotting

Western blot analysis of extracts from human and mouse skeletal muscle tissues using FXR1 (D10A2) XP® Rabbit mAb.

Western blot方法检测人和小鼠骨骼肌的组织提取物,使用的抗体为 FXR1 (D10A2) XP® Rabbit mAb兔单抗。


Fragile X syndrome is a genetic disorder characterized by a spectrum of physical and behavioral features and is a frequent form of inherited mental retardation (1). X-linked FMRP (FMR-1) and its two autosomal homologs, FXR1 and FXR2, are polyribosome-associated RNA-binding proteins that are involved in the pathogenesis of fragile X syndrome (1-3). Each of the fragile X proteins can self-associate, as well as form heteromers with the other two related proteins (3). FMRP can act as a translation regulator and is a component of RNAi effector complexes (RISC), suggesting a role in gene silencing (4). The Drosophila homolog of FMRP (dFMRP) associates with Argonaute 2 (Ago2) and Dicer and can coimmunoprecipitate with miRNA and siRNA (5). These results suggest that fragile X syndrome is related to abnormal translation caused by defects in RNAi-related pathways. In addition, FMRP, FXR1, and FXR2 are components of stress granules (SG) and have been implicated in the translational regulation of mRNAs (6).

X染色体易损综合征(Fragile X syndrome)是以一系列生理、行为学特征为特点一种遗传性紊乱,其也是常见的遗传性精神发育迟疾病(1)。X-linked FMRP (FMR-1) 和它的两个常染色体同系物 (FXR1 and FXR2) 都是多核糖体相关的RNA结合蛋白,它们都涉及到fragile X syndrome的发病机制(1-3)。每一个fragile X蛋白都能自我发生联系,也可以与其它两个相关蛋白形成异源二聚体(3)。FMRP能够作为一种翻译调节因子,同时也是RNAi因子复合物(RISC)的组成部分,研究认为该蛋白在基因沉默中发挥作用(4)。在果蝇中,dFMRP与 Argonaute 2 (Ago2)、Dicer和miRNA及siRNA免疫共沉淀物相联系(5)。这些研究结果认为X染色体易损综合征是和由RNAi相关通路的缺陷而引起非正常的蛋白质翻译相关。此外,FMRP、FXR1和FXR2是应激颗粒(SG)组成成员,同时这些蛋白也涉及到mRNAs翻译的调节(6)。

  1. Verkerk, A.J. et al. (1991) Cell 65, 905-14.
  2. Siomi, M.C. et al. (1995) EMBO J 14, 2401-8.
  3. Zhang, Y. et al. (1995) EMBO J 14, 5358-66.
  4. Caudy, A.A. et al. (2002) Genes Dev 16, 2491-6.
  5. Siomi, H. et al. (2004) Ment Retard Dev Disabil Res Rev 10, 68-74.
  6. Linder, B. et al. (2008) Hum Mol Genet 17, 3236-46.

Application References

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