Cell Signaling Technology

Product Pathways - Development

TEAD1 (D9X2L) Rabbit mAb #12292

No. Size Price
12292S 100 µl ( 10 western blots ) ¥3,100.00 现货查询 购买询价
12292 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Monkey, Endogenous 50 Rabbit IgG
IP 1:100

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation,


Species predicted to react based on 100% sequence homology: Rat,

Specificity / Sensitivity

TEAD1 (D9X2L) Rabbit mAb recognizes endogenous levels of total TEAD1 protein.

TEAD1 (D9X2L)Rabbit mAb兔单抗可以识别内源性的总TEAD1蛋白。

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala185 of human TEAD1 protein.


Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using TEAD1 (D9X2L) Rabbit mAb.

使用TEAD1 (D9X2L)Rabbit mAb兔单抗对多种细胞提取物进行western blot分析。

Western Blotting

Western Blotting

Western blot analysis of extracts from COS-7 cells transfected with a construct expressing either Myc/DDK-tagged full-length human TEAD1 (hTEAD1-Myc/DDK;+), Myc/DDK-tagged full-length human TEAD2 (hTEAD2-Myc/DDK;+), Myc/DDK-tagged full-length human TEAD3 (hTEAD3-Myc/DDK;+), or Myc/DDK-tagged full-length human TEAD4 (hTEAD4-Myc/DDK;+), using TEAD1 (D9X2L) Rabbit mAb #12292 (A), TEAD2 Antibody #8870 (B), TEAD3 Antibody (C), or Pan-TEAD (D3F7L) Rabbit mAb #13295 (D).

COS-7细胞中转染Myc/DDK-tagged 人全长 TEAD1 (hTEAD1-Myc/DDK;+), Myc/DDK-tagged 人全长 TEAD2 (hTEAD2-Myc/DDK;+), Myc/DDK-tagged 人全长 TEAD3 (hTEAD3-Myc/DDK;+), or Myc/DDK-tagged 人全长 TEAD4 (hTEAD4-Myc/DDK;+),使用TEAD1 (D9X2L) Rabbit mAb兔单抗 #12292 (A), TEAD2 Antibody #8870 (B), TEAD3 Antibody (C)或 Pan-TEAD (D3F7L) Rabbit mAb 兔单抗#13295 (D)等抗体对细胞裂解液进行Western blot分析。


The Hippo pathway is an important evolutionarily conserved signaling pathway that controls organ size and tumor suppression by inhibiting cell proliferation and promoting apoptosis (1,2). An integral function of the Hippo pathway is to repress the activity of Yes-associated protein (YAP), a proposed oncogene whose activity is regulated by phosphorylation and subcellular localization (3,4). When the Hippo pathway is turned off, YAP is phosphorylated and translocates to the nucleus where it associates with various transcription factors including members of the transcriptional enhancer factor (TEF) family, also known as the TEA domain (TEAD) family (TEAD1-4) (5,6). Although widely expressed in tissues, the TEAD family proteins have specific tissue and developmental distributions. YAP/TEAD complexes regulate the expression of genes involved in cell proliferation and apoptosis (5).

Hippo通路是一个重要的进化保守的信号通路,通过抑制细胞增殖促进凋亡以控制器官的大小和抑制肿瘤(1,2)。Hippo通路的完整功能是抑制Yes-相关蛋白(YAP)的活性,这是一个致癌基因,其活性受到磷酸化和亚细胞定位的调控(3,4)。当Hippo通路被关闭后,YAP被磷酸化并转位到细胞核,YAP与多种转录因子包括转录增强因子(TEF)家族相互作用,例如TEA结构域(TEAD)家族(TEAD1-4) (5,6).尽管在组织中表达广泛,TEAD家族蛋白有组织特异性和发育特异性。YAP/TEAD复合物调控细胞增殖和凋亡过程中的基因表达(5)。

  1. Pan, D. (2010) Dev Cell 19, 491-505.
  2. Harvey, K.F. et al. (2003) Cell 114, 457-67.
  3. Zhao, B. et al. (2010) Genes Dev 24, 862-74.
  4. Zhao, B. et al. (2008) Curr Opin Cell Biol 20, 638-46.
  5. Zhao, B. et al. (2008) Genes Dev 22, 1962-71.
  6. Zhao, B. et al. (2007) Genes Dev 21, 2747-61.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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