Cell Signaling Technology

Product Pathways - NF-kB Signaling

CARD9 Antibody (Mouse Preferred) #12283

No. Size Price
12283S 100 µl ( 10 western blots ) ¥3,250.00 现货查询 购买询价 防伪查询
12283 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Mouse, Endogenous 64 Rabbit
IP 1:100

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation,

Specificity / Sensitivity

CARD9 Antibody (Mouse Preferred) recognizes endogenous levels of total CARD9 protein.


Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding His478 of mouse CARD9 protein. Antibodies are purified by protein A and peptide affinity chromatography.


Western Blotting

Western Blotting

Western blot analysis of extracts from Raw 264.7 and JAWSII cells using CARD9 Antibody (Mouse Preferred). Western blot分析RAW264.7和JAWSII细胞的细胞提取物,使用抗体是CARD9 Antibody (Mouse Preferred).



Immunoprecipitation of CARD9 from Raw 264.7 cell extracts using Normal Rabbit IgG #2729 (lane 2) or CARD9 Antibody (Mouse Preferred) (lane 3). Lane 1 is 10% input. Western blot analysis was performed using CARD9 Antibody (Mouse Preferred). 对RAW264.7细胞的细胞提取物中的CARD9进行免疫共沉淀,使用抗体是Normal Rabbit IgG #2729 (泳道2)或 CARD9 Antibody (Mouse Preferred) (泳道 3)。泳道1为10%的加入量。Western blot 分析使用抗体是 CARD9 Antibody (Mouse Preferred)。


CARD9 is a caspase recruitment domain (CARD)-containing adaptor protein expressed by myeloid cells (1,2). It is required for antifungal immunity downstream of pathogen detection by C-type lectin receptors (CLRs) such as Dectin-1 (3,4). Recognition of carbohydrates on fungal cell walls by CLRs leads to activation of the tyrosine kinase Syk, followed by activation of PKCδ (5,6). PKCδ phosphorylates CARD9, enabling the assembly of a complex containing CARD9 and Bcl10 (6). This complex activates NF-κB, resulting in upregulation of inflammatory cytokines important for initiation of adaptive immunity (3,4,6,7). CARD9 was also shown to be important for the induction of IL-1β, downstream of the viral nucleic acid sensor RIG-I, as well as for the generation of reactive oxygen species important for bacterial killing by macrophages (2,8).


  1. Bertin, J. et al. (2000) J Biol Chem 275, 41082-6.
  2. Hsu, Y.M. et al. (2007) Nat Immunol 8, 198-205.
  3. Gross, O. et al. (2006) Nature 442, 651-6.
  4. Bi, L. et al. (2010) J Biol Chem 285, 25969-77.
  5. Rogers, N.C. et al. (2005) Immunity 22, 507-17.
  6. Strasser, D. et al. (2012) Immunity 36, 32-42.
  7. LeibundGut-Landmann, S. et al. (2007) Nat Immunol 8, 630-8.
  8. Wu, W. et al. (2009) Nat Immunol 10, 1208-14.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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