Cell Signaling Technology

Product Pathways - NF-kB Signaling

Toll-like Receptor 2 (D7G9Z) Rabbit mAb #12276

sc-10739  

No. Size Price
12276S 100 µl ( 10 western blots ) ¥3,100.00 现货查询 购买询价
12276 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human, Endogenous 90-105 Rabbit IgG
IP 1:100

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation,

Specificity / Sensitivity

Toll-like Receptor 2 (D7G9Z) Rabbit mAb recognizes endogenous levels of total TLR2 protein.

Toll-like Receptor 2 (D7G9Z) Rabbit mAb 兔单抗能够检测内源性TLR2总蛋白水平。

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val303 of human TLR2 protein.

此单克隆抗体通过合成肽免疫动物制备,这种合成肽是人源TLR2蛋白Val303周围的肽段。

Western Blotting

Western Blotting

Western blot analysis of THP-1 and MUTZ-3 cells using Toll-like Receptor 2 (D7G9Z) Rabbit mAb. Western blot 分析THP-1和MUTZ-3细胞的细胞提取物,使用抗体是Toll-like Receptor 2 (D7G9Z) Rabbit mAb。

Western Blotting

Western Blotting

Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with a construct expressing HA-tagged full-length human TLR2 (hTLR2-HA; +), using Toll-like Receptor 2 (D7G9Z) Rabbit mAb. Western blot分析293T细胞的细胞提取物,对照转染(-)或转染表达HA标签全长人源TLR2的构建质粒(hTLR2-HA; +), 使用抗体是Toll-like Receptor 2 (D7G9Z) Rabbit mAb。

IP

IP

Immunoprecipitation of TLR2 from MUTZ-3 cell extracts using Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) or Toll-like Receptor 2 (D7G9Z) Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using Toll-like Receptor 2 (D7G9Z) Rabbit mAb. 对MUTZ-3细胞中的TLR2进行免疫沉淀,使用抗体是Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (泳道2) 或 Toll-like Receptor 2 (D7G9Z) Rabbit mAb (泳道3)。泳道1为10%的加入量。Western blot分析使用抗体是 Toll-like Receptor 2 (D7G9Z) Rabbit mAb。

Background

Members of the Toll-like receptor (TLR) family, named for the closely related Toll receptor in Drosophila, play a pivotal role in innate immune responses (1-4). TLRs recognize conserved motifs found in various pathogens and mediate defense responses (5-7). Triggering of the TLR pathway leads to the activation of NF-κB and subsequent regulation of immune and inflammatory genes (4). The TLRs and members of the IL-1 receptor family share a conserved stretch of approximately 200 amino acids known as the Toll/Interleukin-1 receptor (TIR) domain (1). Upon activation, TLRs associate with a number of cytoplasmic adaptor proteins containing TIR domains, including myeloid differentiation factor 88 (MyD88), MyD88-adaptor-like/TIR-associated protein (MAL/TIRAP), Toll-receptor-associated activator of interferon (TRIF), and Toll-receptor-associated molecule (TRAM) (8-10). This association leads to the recruitment and activation of IRAK1 and IRAK4, which form a complex with TRAF6 to activate TAK1 and IKK (8,11-14). Activation of IKK leads to the degradation of IκB, which normally maintains NF-κB in an inactive state by sequestering it in the cytoplasm.Toll样受体(TLR) 的家族成员, 是以与其密切相关的果蝇Toll受体而命名的,TLR在先天免疫中发挥了极其重要的作用(1-3)。TLRs 能够识别不同病原菌中保守的结构域并介导防御应答。TLR的触发导致了NF-κB信号通路的激活并因此调控下游的免疫和炎症基因的表达。TLRs与IL-1受体家族都有一个大约200个氨基酸的保守区域叫做TIR域。被激活后, TLRs与细胞质中的含有TIR区域的一系列接头蛋白作用,包括MyD88 (骨髓分化因子), MAL/TIRAP (MyD88-adaptor-like/TIR-associated 蛋白), TRIF (Toll-receptor相关的干扰素激活物)和 TRAM (Toll-receptor相关的分子).。这些反应导致了IRAK1 和 IRAK4的招募和激活并与TRAF6 形成复合体激活 TAK1 和 IKK。IKK的激活导致了因锚定NF-κB在细胞质中而使其失去活性的IκB的降解。

TLR2 is expressed on the surface of monocytes and macrophages and can heterodimerize with TLR1 or TLR6, enabling responses to a variety of pathogen-associated molecular patterns including lipopetides and peptidoglycan (15-18).

TLR2表达于单核细胞和巨噬细胞的表面,能够与TLR1或TLR6形成异源二聚体,并且能够对各种病原相关分子模式产生应答,包括脂肽和肽聚糖(15,18)。

  1. Akira, S. (2003) J Biol Chem 278, 38105-8.
  2. Beutler, B. (2004) Nature 430, 257-63.
  3. Dunne, A. and O'Neill, L.A. (2003) Sci STKE 2003, re3.
  4. Medzhitov, R. et al. (1997) Nature 388, 394-7.
  5. Schwandner, R. et al. (1999) J Biol Chem 274, 17406-9.
  6. Takeuchi, O. et al. (1999) Immunity 11, 443-51.
  7. Alexopoulou, L. et al. (2001) Nature 413, 732-8.
  8. Zhang, F.X. et al. (1999) J Biol Chem 274, 7611-4.
  9. Horng, T. et al. (2001) Nat Immunol 2, 835-41.
  10. Oshiumi, H. et al. (2003) Nat Immunol 4, 161-7.
  11. Muzio, M. et al. (1997) Science 278, 1612-5.
  12. Wesche, H. et al. (1997) Immunity 7, 837-47.
  13. Suzuki, N. et al. (2002) Nature 416, 750-6.
  14. Irie, T. et al. (2000) FEBS Lett 467, 160-4.
  15. Takeuchi, O. et al. (2002) J Immunol 169, 10-4.
  16. Schwandner, R. et al. (1999) J Biol Chem 274, 17406-9.
  17. Lien, E. et al. (1999) J Biol Chem 274, 33419-25.
  18. Ozinsky, A. et al. (2000) Proc Natl Acad Sci U S A 97, 13766-71.

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Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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