Cell Signaling Technology

Product Pathways - Chromatin Regulation / Epigenetics

TH1L (D5G6W) Rabbit mAb #12265

No. Size Price
12265S 100 µl ( 10 western blots ) ¥3,100.00 现货查询 购买询价
12265 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat,Monkey, Endogenous 66 Rabbit IgG
IP 1:100
ChIP 1:100

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation, ChIP=Chromatin IP,


Species predicted to react based on 100% sequence homology: Hamster, Bovine, Dog, Horse,

Specificity / Sensitivity

TH1L (D5G6W) Rabbit mAb recognizes endogenous levels of total TH1L protein.

TH1L (D5G6W) Rabbit mAb 兔单抗能够检测内源性TH1L总蛋白水平。

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala132 of human TH1L protein.

该单克隆抗体经由合成的围绕人TH1L蛋白 la1325位点周围的氨基酸肽段免疫动物而生产的。

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using TH1L (D5G6W) Rabbit mAb.Western blot方法检测不同细胞系的提取物,使用的抗体为 TH1L (D5G6W) Rabbit mAb。



Immunoprecipitation of TH1L from HCT 116 cell extracts using Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) or TH1L (D5G6W) Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using TH1L (D5G6W) Rabbit mAb.从 HCT 116细胞提取物中免疫沉淀TH1L,使用的抗体为Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2)或 TH1L (D5G6W) Rabbit mAb (lane 3)。Lane 1为10% input。 使用TH1L (D5G6W) Rabbit mAb进行Western blot检测。

Chromatin IP

Chromatin IP

Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 106 MCF7 cells grown in phenol red-free medium and 5% charcoal-stripped FBS for 4 d and either 5 μl of TH1L (D5G6W) Rabbit mAb or 2 μl of Normal Rabbit IgG #2729 using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using SimpleChIP® Human PMAIP1 Intron 1 Primers #12558, human c-Myc intron 1 primers, and SimpleChIP® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.使用从4 x 106 HCT 116细胞中提取的交联过的染色质,以及5 µl TH1L (D5G6W) Rabbit mAb 或2 µl Normal Rabbit IgG #2729进行染色质免疫沉淀,这些细胞含5%活性炭处理胎牛血清以及无酚红的培养基中培养4天。所用试剂盒为SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003。富集到的DNA经过实时PCR定量,所使用引物为SimpleChIP® Human PMAIP1 Intron 1 Primers #12558, human c-Myc intron 1 primers,以及 SimpleChIP® Human α Satellite Repeat Primers #4486。每个样品中免疫沉淀得到的DNA量由与input chromatin( 相当于1)的相对信号值来表示。


Negative Elongation Factor (NELF) consists of four subunits: WHSC2 (NELF-A), COBRA-1 (NELF-B), TH1L (NELF-C/D), and NELF-E (1). NELF, together with DRB-sensitivity inducing factor (DSIF), inhibits RNA Polymerase II (RNAPII) elongation resulting in RNAPII promoter proximal pausing, where it awaits additional signaling to resume transcription (2,3). The release of RNAPII from promoter proximal pausing is a critical regulatory point during transcription and is signaled by positive transcription elongation factor (p-TEF-b) phosphorylation of both NELF and the C-terminal domain (CTD) within the largest subunit of RNAPII (4,5). WHSC2 is thought to connect the NELF complex to RNAPII machinery, while NELF-E contains an RNA binding motif that is necessary for NELF function (1,6,7). TH1L, together with COBRA-1, are integral subunits that bring WHSC2 and NELF-E together in the NELF complex (1).

Negative Elongation Factor (NELF)由四个亚基组成:WHSC2 (NELF-A)、COBRA-1 (NELF-B)、TH1L (NELF-C/D)和NELF-E (1)。NELF蛋白与DRB-sensitivity inducing factor (DSIF)一起抑制RNA Polymerase II (RNAPII)延伸,这导致RNAPII启动子近侧间歇(proximal pausing),在启动子上等待另外信号去重新开始转录(2,3)。在转录期间,RNAPII从启动子近端暂停的释放是转录过程中一个关键的调控点,这个释放过程是通过NELF和RNAPII最大亚基上的C-terminal domain (CTD) 的positive transcription elongation factor (p-TEF-b)磷酸化(4,5)来发出信号的。WHSC2被认为可将NELF连接到RNAPII装置,而NELF-E包含一个RNA结合域,该结构域是NELF行使功能所必需的(1,6,7)。TH1L与COBRA-1一起,是使NELF复合物中WHSC2和NELF-E在一起的不可缺少的亚基 (1)。

  1. Narita, T. et al. (2003) Mol Cell Biol 23, 1863-73.
  2. Nechaev, S. and Adelman, K. (2011) Biochim Biophys Acta 1809, 34-45.
  3. Yamaguchi, Y. et al. (1999) Cell 97, 41-51.
  4. Buratowski, S. (2009) Mol Cell 36, 541-6.
  5. Yamaguchi, Y. et al. (2001) Science 293, 124-7.
  6. Yamaguchi, Y. et al. (2002) Mol Cell Biol 22, 2918-27.

Application References

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