Cell Signaling Technology

Product Pathways - Lymphocyte Signaling

TAP2 Antibody #12259

No. Size Price
12259S 100 µl ( 10 western blots ) ¥3,250.00 现货查询 购买询价 防伪查询
12259 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human, Endogenous 72 Rabbit
IP 1:50

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation,


Species predicted to react based on 100% sequence homology: Monkey,

Specificity / Sensitivity

TAP2 Antibody recognizes endogenous levels of total TAP2 protein.


Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Phe588 of human TAP2 protein. Antibodies are purified by protein A and peptide affinity chromatography.


Western Blotting

Western Blotting

Western blot analysis of extracts from HeLa and HT-29 cells, untreated (-) or treated with Human Interferon-γ (hIFN-γ) #8901 (10 ng/ml, 16 hr; +), using TAP2 Antibody (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower).Western blot分析HeLa和HT-29细胞的提取物,未处理(-)或用Human Interferon-γ (hIFN-γ) #8901 (10 ng/ml, 16 小时; +)处理。使用的抗体是:TAP2 Antibody(上图)、β-Actin (D6A8) Rabbit mAb #8457(下图)。



Immunoprecipitation of TAP2 from HeLa cell extracts treated with Human Interferon-γ (hIFN-γ) #8901 (10 ng/ml, 16 hr), using Normal Rabbit IgG #2729 (lane 2) or TAP2 Antibody (lane 3). Lane 1 is 10% input. Western blot analysis was performed using TAP2 Antibody.免疫沉淀分析来源于HeLa细胞提取物的TAP2,用Human Interferon-γ (hIFN-γ) #8901 (10 ng/ml, 16 小时)处理。使用的抗体是:Normal Rabbit IgG #2729(泳道2)、TAP2 Antibody(泳道3)。泳道1是10% input。Western blot分析使用的抗体是TAP2 Antibody。  


CD8+ cytotoxic T cells recognize peptides presented by MHC class I molecules on the surface of infected cells and tumor cells. The transporters associated with antigen processing 1 and 2 (TAP1 and TAP2) form the TAP complex which resides on the ER membrane and transports peptides from the cytoplasm into the ER for loading onto MHC class I molecules (1-8). In addition, TAP localized to endosomal membranes is important for cross-presentation by dendritic cells (9,10). IFN-γ produced by T cells and NK cells in response to infection causes upregulation of TAP1 and TAP2, resulting in increased antigen presentation to T cells (11). Some viral proteins inhibit TAP function or downregulate TAP expression resulting in viral immune evasion (12,13). In addition, investigators have observed reduced TAP expression in a variety of tumor types, and it is thought to be one mechanism for tumor immune evasion (14).

CD8+细胞毒性T细胞能识别由感染细胞和肿瘤细胞表面的MHC I类分子提呈的肽类。抗原提呈运转运蛋白1和2(TAP1 and TAP2)形成TAP复合物,位于内质网膜,能将肽类从细胞质运输到内质网并装配到MHC I类分子上(1-8)。另外,位于胞内体膜的TAP对树突状细胞的交叉提呈有重要作用(9,10)。由T细胞和NK细胞产生的应对感染的IFN-γ能引起TAP1和TAP2的上调,导致抗原提呈到T细胞的增加(11)。一些病毒蛋白能抑制TAP功能或下调TAP的表达,导致病毒的免疫逃避(12,13)。此外,研究者在许多肿瘤类型中都观察到了TAP表达的减少,并认为这可能是肿瘤免疫逃避的机制之一(14)。

  1. Trowsdale, J. et al. (1990) Nature 348, 741-4.
  2. Spies, T. et al. (1990) Nature 348, 744-7.
  3. Deverson, E.V. et al. (1990) Nature 348, 738-41.
  4. Monaco, J.J. et al. (1990) Science 250, 1723-6.
  5. Spies, T. and DeMars, R. (1991) Nature 351, 323-4.
  6. Kleijmeer, M.J. et al. (1992) Nature 357, 342-4.
  7. Kelly, A. et al. (1992) Nature 355, 641-4.
  8. Spies, T. et al. (1992) Nature 355, 644-6.
  9. Huang, A.Y. et al. (1996) Immunity 4, 349-55.
  10. Guermonprez, P. et al. (2003) Nature 425, 397-402.
  11. Bahram, S. et al. (1991) Proc Natl Acad Sci U S A 88, 10094-8.
  12. Früh, K. et al. (1995) Nature 375, 415-8.
  13. Bennett, E.M. et al. (1999) J Immunol 162, 5049-52.
  14. Steer, H.J. et al. (2010) Oncogene 29, 6301-13.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

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