Cell Signaling Technology

Product Pathways - Apoptosis

Lamin B2 (D8P3U) Rabbit mAb #12255

sc-56144   sc-6216  

No. Size Price
12255S 100 µl ( 10 western blots ) ¥3,100.00 现货查询 购买询价
12255 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Monkey, Endogenous 68 Rabbit IgG
IP 1:100
IF-IC 1:50

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation, IF-IC=Immunofluorescence (Immunocytochemistry),

Specificity / Sensitivity

Lamin B2 (D8P3U) Rabbit mAb recognizes endogenous levels of total lamin B2 protein.

Lamin B2 (D8P3U) Rabbit mAb 兔单抗能够检测内源性的 lamin B2 总蛋白。

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Lys415 of human lamin B2 protein.

该单克隆抗体是采用合成的人源lamin B2蛋白Lys415 周围残基相对应的肽段免疫动物而制备的。抗体由protein A和肽亲和层析技术纯化。



Confocal immunofluorescent analysis of HCT 116 cells using Lamin B2 (D8P3U) Rabbit mAb (green). Actin filaments were labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

共聚焦免疫荧光分析HCT 116 细胞,使用抗体为 Lamin B2 (D8P3U) Rabbit mAb 兔单抗(绿色)。DY-554 phalloidin(红色)标记肌动蛋白丝。蓝色伪彩=DRAQ5®#4084(荧光DNA染料)。

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using Lamin B2 (D8P3U) Rabbit mAb.

Western blot 检测多种细胞系提取物,使用抗体为 Lamin B2 (D8P3U) Rabbit mAb 兔单抗。



Immunoprecipitation of lamin B2 from Jurkat cell extracts, using Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) or Lamin B2 (D8P3U) Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using Lamin B2 (D8P3U) Rabbit mAb.

免疫沉淀方法检测Jurkat 细胞提取物中 lamin B2 ,抗体为Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (泳道2)或Lamin B2 (D8P3U) Rabbit mAb 兔单抗(泳道3)。泳道1为10%对照。使用Lamin B2 (D8P3U) Rabbit mAb 兔单抗进行Western blot分析。


Lamins are nuclear membrane structural components that are important in maintaining normal cell functions, such as cell cycle control, DNA replication, and chromatin organization (1-3). Lamins have been subdivided into types A and B. Type-A lamins consist of lamin A and C, arising from alternative splicing. Lamin A and C are cleaved by caspases into large (41-50 kDa) and small (28 kDa) fragments, which can be used as markers for apoptosis (4,5). Type-B lamins consist of lamin B1 and B2, encoded by separate genes (6-8). Lamin B1 is also cleaved by caspases during apoptosis (9). Research studies have shown that duplication of the lamin B1 gene is correlated with the pathogenesis of the neurological disorder adult-onset leukodystrophy (10).

核纤层蛋白Lamin是核膜结构的组分,对维持正常的细胞功能非常重要,例如细胞周期控制、DNA修复和染色质组装(1-3)。Lamins 已细分为A和B型。A型核纤层蛋白由核纤层蛋白A和C组成,来源于选择性剪接。Lamin A/C 能够被剪切成特异的大片段(41-50 kDa)和小片段 (28 kDa) ,这是细胞凋亡的标志(4,5)。B型核纤层蛋白由核纤层蛋白B1和B2组成,由单独的基因编码(6-8)。在细胞凋亡时,核纤层蛋白B1也被半胱天冬酶酶切(9)。研究显示,核纤层蛋白B1基因的复制,与神经功能紊乱并在成年期发病的脑白质病变的发病机制相关(10)。

Lamin B2 knockout mice have defects in neuronal development (11) that are distinct from lamin B1 knockout mice (12).


  1. Gruenbaum, Y. et al. (2000) J Struct Biol 129, 313-23.
  2. Goldberg, M. et al. (1999) Crit Rev Eukaryot Gene Expr 9, 285-93.
  3. Yabuki, M. et al. (1999) Physiol Chem Phys Med NMR 31, 77-84.
  4. Rao, L. et al. (1996) J Cell Biol 135, 1441-55.
  5. Orth, K. et al. (1996) J Biol Chem 271, 16443-6.
  6. Biamonti, G. et al. (1992) Mol Cell Biol 12, 3499-506.
  7. Lin, F. and Worman, H.J. (1995) Genomics 27, 230-6.
  8. Pollard, K.M. et al. (1990) Mol Cell Biol 10, 2164-75.
  9. Chandler, J.M. et al. (1997) Biochem J 322 ( Pt 1), 19-23.
  10. Padiath, Q.S. et al. (2006) Nat Genet 38, 1114-23.
  11. Coffinier, C. et al. (2010) Proc Natl Acad Sci U S A 107, 5076-81.
  12. Coffinier, C. et al. (2011) Mol Biol Cell 22, 4683-93.
  13. Hegele, R.A. et al. (2006) Am J Hum Genet 79, 383-9.

Application References

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