Cell Signaling Technology

Product Pathways - Protein Stability

Skp1 (D3J4N) Rabbit mAb #12248

E3 Ligase   Skp-1A   Skp-1B   Skp1A   Skp1B  

No. Size Price
12248S 100 µl ( 10 western blots ) ¥3,250.00 现货查询 购买询价 防伪查询
12248 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat,Monkey, Endogenous 19 Rabbit IgG
IP 1:100

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation,


Species predicted to react based on 100% sequence homology: Zebrafish,

Specificity / Sensitivity

Skp1 (D3J4N) Rabbit mAb recognizes endogenous levels of total Skp1 protein.

Skp1 (D3J4N) Rabbit mAb兔单抗识别内源性的Skp1总蛋白。

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human Skp1 protein.


Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using Skp1 (D3J4N) Rabbit mAb.Western blot检测多种细胞提取物。

Western Blotting

Western Blotting

Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with a construct expressing Myc/DDK-tagged full-length human Skp1 isoform b (hSkp1-Myc/DDK; +), using Skp1 (D3J4N) Rabbit mAb.Western blot检测mock transfected (-)或转染有结构性表达Myc/DDK-tagged人全长Skp1同型异构体b (hSkp1-Myc/DDK; +)的293T细胞,采用 Skp1 (D3J4N) Rabbit mAb。



Immunoprecipitation of Skp1 from 293T cell extracts using Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) or Skp1 (D3J4N) Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using Skp1 (D3J4N) Rabbit mAb.Immunoprecipitation检测293T细胞提取物中Skp1,采用Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) 或Skp1 (D3J4N) Rabbit mAb (lane 3)。Lane 1中样品浓度为10%。Western blot检测采用Skp1 (D3J4N) Rabbit mAb。


Ubiquitin can be covalently linked to many cellular proteins by the ubiquitination process, which targets proteins for degradation by the 26S proteasome. Three components are involved in the target protein-ubiquitin conjugation process. Ubiquitin is first activated by forming a thiolester complex with the activation component E1; the activated ubiquitin is subsequently transferred to the ubiquitin-carrier protein E2 and then from E2 to ubiquitin ligase E3 for final delivery to the epsilon-NH2 of the target protein lysine residue (1-3). Combinatorial interactions of different E2 and E3 proteins result in substrate specificity (4). Recent data suggests that activated E2 associates transiently with E3, and the dissociation is a critical step for ubiquitination (5). S phase kinase-associated protein 1 (Skp1) is a critical scaffold protein of the Skp1/CUL1/F-box (SCF) E3 ubiquitin ligase protein complex. Various F-box proteins (e.g., β-TrCP, Skp2) mediate an interaction with Skp1, via their defining and conserved domain of 40 amino acids, and with substrates to be ubiquitinated (e.g., β-catenin, p27) (4).

泛素通过泛素化作用可以与许多细胞蛋白共价连接,这些蛋白可以被26S蛋白酶体降解。3种组分包含在蛋白-泛素共价连接过程。泛素首先通过形成具有活化组分E1的硫酯复合物被激活;活化的泛素随后转运到泛素载体蛋白E2,随后从E2到泛素连接酶E3进行最后的转运到epsilon-NH2的靶蛋白赖氨酸残基(1-3)。不同E2和E3蛋白的相互作用导致底物的特异性(4)。最近的数据表明活化的E2短暂地与E3相关,解离是去泛素化的重要步骤(5)。S期激酶相关蛋白(Skp1)是Skp1/CUL1/F-box (SCF) E3泛素连接酶蛋白复合物的一种重要的支架蛋白。多种F-box蛋白(例如:β-TrCP, Skp2)介导与Skp1的相互作用,经它们确定的40氨基酸的保守结构域,与被泛素化的底物相互作用(例如:β-catenin, p27) (4)。

  1. Ciechanover, A. (1998) EMBO J. 17, 7151-60.
  2. Hochstrasser, M. (2000) Nat. Cell Biol. 2, E153-7.
  3. Hochstrasser, M. (2000) Science 289, 563-4.
  4. DeSalle, L.M. and Pagano, M. (2001) FEBS Lett. 490, 179-89.
  5. Deffenbaugh, A.E. et al. (2003) Cell 114, 611-22.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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