Cell Signaling Technology

Product Pathways - Cell Cycle / Checkpoint

TPX2 (D2R5C) XP® Rabbit mAb #12245

No. Size Price
12245S 100 µl ( 10 western blots ) ¥3,580.00 现货查询 购买询价
12245 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Monkey, Endogenous 100 Rabbit IgG
IP 1:100
IF-IC 1:1600

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation, IF-IC=Immunofluorescence (Immunocytochemistry),

Specificity / Sensitivity

TPX2 (D2R5C) XP® Rabbit mAb recognizes endogenous levels of total TPX2 protein.TPX2 (D2R5C) XP® Rabbit mAb可以识别内源性TPX2总蛋白。

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Lys420 of human TPX2 protein.单克隆抗体由合成肽段免疫动物产生,该肽段与人TPX2的420位赖氨酸邻近残基序列一致。

IF-IC

IF-IC

Confocal immunofluorescent analysis of MCF7 cells using TPX2 (D2R5C) XP® Rabbit mAb (green). Actin filaments were labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).使用TPX2 (D2R5C) XP®Rabbit mAb(绿色)对MCF7细胞进行激光共聚焦免疫荧光分析。肌动蛋白丝用DY-554鬼笔环肽标记(红色)。蓝色假色= DRAQ5® #4084 (fluorescent DNA dye).

IP

IP

Immunoprecipitation of TPX2 from MCF7 cell extracts using Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) or TPX2 (D2R5C) XP® Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using TPX2 (D2R5C) XP® Rabbit mAb.使用Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) 或使用TPX2 (D2R5C) XP®Rabbit mAb(lane 3)。Lane1使用10%上样量。使用TPX2 (D2R5C) XP®Rabbit mAb进行western blot分析。

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using TPX2 (D2R5C) XP® Rabbit mAb.使用TPX2 (D2R5C) XP® Rabbit mAb对多种细胞提取物进行western blot分析。

Background

The Ras family small GTPase Ran is involved in nuclear envelope formation, assembly of the mitotic spindle, and nuclear transport (1,2). TPX2, a target of active Ran (RanGTP), is a microtubule nucleating protein. TPX2 is inactive when bound to nuclear importin-alpha. RanGTP activity disrupts this interaction, relieving inhibition of TPX2 (3). TPX2 binding activates Aurora A kinase and promotes its localization to the mitotic spindle (4,5). DNA damage in mitosis leads to loss of interaction between Aurora A and TPX2 and inactivation of Aurora A kinase (6). TPX2 is highly expressed in pancreatic cancer cells, and knockdown of TPX2 expression in these cells is associated with increased sensitivity to paclitaxel (7).核膜形成,有丝分裂纺锤体的聚集以及核转运过程都涉及了Ras家族小GTPase Ran蛋白(1.2)。TPX2,活化Ran(RanGTP)的一个靶蛋白是一个微管核形成蛋白。TPX2与细胞核输入-alpha结合时是失活状态。RanGTP的活性能够破坏这种结合,减弱TPX2的抑制作用(3)。TPX2的结合会激活Aurora A激酶促进其定位到有丝分裂的纺锤体(4,5)。有丝分裂时DNA损伤会丢失Aurora A和TPX2的结合从而使Aurora 激酶失活(6)。TPX2在胰腺癌细胞中高表达,敲除TPX2会增强细胞对紫杉醇的敏感性(7)。

  1. Quimby, B.B. and Dasso, M. (2003) Curr Opin Cell Biol 15, 338-44.
  2. Hetzer, M. et al. (2002) Nat Cell Biol 4, E177-84.
  3. Gruss, O.J. and Vernos, I. (2004) J Cell Biol 166, 949-55.
  4. Kufer, T.A. et al. (2002) J Cell Biol 158, 617-23.
  5. Bayliss, R. et al. (2004) Cell Cycle 3, 404-7.
  6. Bhatia, P. et al. (2010) Cell Cycle 9, 4592-9.
  7. Warner, S.L. et al. (2009) Clin Cancer Res 15, 6519-28.

Application References

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!

Protocols

Companion Products


For Research Use Only. Not For Use In Diagnostic Procedures.

DRAQ5 is a registered trademark of Biostatus Limited.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

XP is a registered trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

用户评论 --- 共 0

该产品暂无评论!

我要参与评论 :

如要参与评论请先登录网站

还没有网站账户?去注册一下吧

Products

 

Applications