Cell Signaling Technology

Product Pathways - NF-kB Signaling

IL-1β (3A6) Mouse mAb #12242

IL-1B   sc-7884  

No. Size Price
12242S 100 µl ( 10 western blots ) ¥3,100.00 现货查询 购买询价
12242 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse, Endogenous 17,31 Mouse IgG1
IHC-P 1:100

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IHC-P=Immunohistochemistry (Paraffin),

Specificity / Sensitivity

IL-1β (3A6) Mouse mAb recognizes endogenous levels of total IL-1β protein.

IL-1β (3A6) Mouse mAb鼠单抗能够检测内源性IL-1β总蛋白水平。

Source / Purification

Monoclonal antibody is produced by immunizing animals with recombinant human IL-1β protein.

此单克隆抗体通过重组人源IL-1β 蛋白免疫动物制备。

Western Blotting

Western Blotting

Western blot analysis of 1 ng recombinant Human Interleukin-1β (hIL-1β) #8900 and 1 ng recombinant Mouse Interleukin-1β (mIL-1β) #5204 using IL-1β (3A6) Mouse mAb. Western blot分析1 ng recombinant Human Interleukin-1β (hIL-1β) #8900 和1 ng recombinant Mouse Interleukin-1β (mIL-1β) #5204,使用抗体是IL-1β (3A6) Mouse mAb。

Western Blotting

Western Blotting

Western blot analysis of extracts from Raw 264.7 cells, untreated (-) or treated with Brefeldin A #9972 (300 ng/mL, last 3 hr of stimulation; +) and LPS (100 ng/mL, 6 hr; +), using IL-1β (3A6) Mouse mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower).Western blot分析Raw264.7细胞的细胞提取物,未处理(-)或使用Brefeldin A #9972 (300 ng/mL, 刺激持续3小时; +) 和 LPS (100 ng/mL, 6 hr; +)处理,使用抗体是IL-1β (3A6) Mouse mAb (上图) 或 β-Actin (D6A8) Rabbit mAb #8457 (下图)。

Western Blotting

Western Blotting

Western blot analysis of extracts from THP-1 cells, untreated (-) or LPS-treated (100 ng/mL, 3 hr; +), using IL-1β (3A6) Mouse mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower). Western blot分析THP-1细胞的细胞提取物,未处理(-)或LPS-处理 (100 ng/mL, 3 小时; +),使用抗体是IL-1β (3A6) Mouse mAb (上图) 或 β-Actin (D6A8) Rabbit mAb #8457 (下图)。

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human large intestine (chronic colitis of the colon) using IL-1-β (3A6) Mouse mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin embedded THP-1 cell pellets, control (left) or LPS-treated (right) using IL-1-β (3A6) Mouse mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human large intestine (ulcerative chronic colitis of the rectum) using IL-1-β (3A6) Mouse mAb.

Background

Interleukin-1β (IL-1β), one of the major caspase-1 targets, is a multifunctional cytokine that is involved in a host of immune and proinflammatory responses (1). It is produced primarily by activated monocytes and macrophages. It signals through various adaptor proteins and kinases that lead to activation of numerous downstream targets (2-6). Human IL-1β is synthesized as a 31 kDa precursor. To gain activity, the precursor must be cleaved by caspase-1 between Asp116 and Ala117 to yield a 17 kDa mature form (7,8). Detection of the 17 kDa mature form of IL-1β is a good indicator of caspase-1 activity.

IL-1β作为caspsase-1的靶分子,是一种具有多种功能的细胞因子,与宿主免疫以及促炎反应有关(1)。它早期是由活化的单核细胞和巨噬细胞产生的,通过多种接头蛋白和激酶发出信号,从而激活许多下游目标(2-6)。人源IL-1β合成为一种31kDa的前导蛋白。为了获得活性,前导蛋白必须通过caspase-1加工,将其在Asp116和Ala117中裂解开,从而得到一个17kDa的成熟形式(7,8)。对这种17kDa的成熟形式的检测是对Caspase-1活性的一种良好的指示物。

  1. Dinarello, C.A. (1998) Int Rev Immunol 16, 457-99.
  2. Burns, K. et al. (1998) J Biol Chem 273, 12203-9.
  3. Cao, Z. et al. (1996) Nature 383, 443-6.
  4. Cao, Z. et al. (1996) Science 271, 1128-31.
  5. Wesche, H. et al. (1997) Immunity 7, 837-47.
  6. Ninomiya-Tsuji, J. et al. (1999) Nature 398, 252-6.
  7. Thornberry, N.A. et al. (1992) Nature 356, 768-74.
  8. Cerretti, D.P. et al. (1992) Science 256, 97-100.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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