Cell Signaling Technology

Product Pathways - Chromatin Regulation / Epigenetics

SignalSilence® SirT1 siRNA I #12241

Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
TFN Human,

Species cross-reactivity is determined by western blot.

Applications Key: TFN=Transfection,


Species predicted to react based on 100% sequence homology: Mouse, Rat, Monkey,

Specificity / Sensitivity

SignalSilence® SirT1 siRNA I inhibits human, mouse, rat, and monkey SirT1 expression.

SignalSilence® SirT1 siRNA I 能够抑制人、小鼠、大鼠以及猴子中SirT1的表达。


SignalSilence® SirT1 siRNA I from Cell Signaling Technology (CST) allows the researcher to specifically inhibit SirT1 expression using RNA interference, a method whereby gene expression can be selectively silenced through the delivery of double stranded RNA molecules into the cell. All SignalSilence® siRNA products from CST are rigorously tested in-house and have been shown to reduce target protein expression by western analysis.

来自Cell Signaling Technology (CST)的SignalSilence® SirT1 siRNA I可以通过RNA干扰特异性抑制SirT1的表达。这种方法可以通过将双链RNA分子传递到细胞内从而使基因表达有选择的沉默。来自CST的所有的SignalSilence®siRNA产品都是经过内部严格检测的,并且通过Western blot 分析证明确实能够减少目的蛋白的表达。

Quality Control

Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.


Western Blotting

Western Blotting

Western blot analysis of extracts from 293T cells, transfected with 100 nM SignalSilence® Control siRNA (Unconjugated) #6568 (-) or SignalSilence® SirT1 siRNA I (+), using SirT1 (D1D7) Rabbit mAb #9475 (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower). The SirT1 (D1D7) Rabbit mAb confirms silencing of SirT1 expression, while the β-Actin (D6A8) Rabbit mAb is used as a loading control.

Western blot 方法检测293T细胞提取物。用100 nM SignalSilence® Control siRNA (Unconjugated) #6568 (-)或SignalSilence® SirT1 siRNA I (+)转染细胞。所用抗体为SirT1 (D1D7) Rabbit mAb #9475 兔单抗(上图) 或 β-Actin (D6A8) Rabbit mAb #8457兔单抗 (下图)。使用SirT1 (D1D7) Rabbit mAb确定SirT1表达的沉默效率,β-Actin (D6A8) 兔单克隆抗体作为上样量对照。

Directions for Use

CST recommends transfection with 100 nM SignalSilence® SirT1 siRNA I 48 to 72 hours prior to cell lysis. For transfection procedure, follow protocol provided by the transfection reagent manufacturer. Please feel free to contact CST with any questions on use. 
 Each vial contains the equivalent of 100 transfections, which corresponds to a final siRNA concentration of 100 nM per transfection in a 24-well plate with a total volume of 300 μl per well.

CST建议使用100 nM SignalSilence® SirT1 siRNA I进行转染,48到72小时后对细胞进行裂解。转染步骤按照转染试剂说明书提供的步骤进行。遇到任何使用方面的问题,请随时联系CST。每小瓶可供100次转染,每次转染量相当于在转染24孔板时,每孔总体积为300μl培养基中siRNA的终浓度为100nM。


The Silent Information Regulator (SIR2) family of genes is a highly conserved group of genes that encode nicotinamide adenine dinucleotide (NAD)-dependent protein deacetylases, also known as class III histone deacetylases. The first discovered and best characterized of these genes is Saccharomyces cerevisiae SIR2, which is involved in silencing of mating type loci, telomere maintenance, DNA damage response, and cell aging (1). SirT1, the mammalian ortholog of Sir2, is a nuclear protein implicated in the regulation of many cellular processes, including apoptosis, cellular senescence, endocrine signaling, glucose homeostasis, aging, and longevity. Targets of SirT1 include acetylated p53 (2,3), p300 (4), Ku70 (5), forkhead (FoxO) transcription factors (5,6), PPARγ (7), and the PPARγ coactivator-1α (PGC-1α) protein (8). Deacetylation of p53 and FoxO transcription factors represses apoptosis and increases cell survival (2,3,5,6). Deacetylation of PPARγ and PGC-1α regulates the gluconeogenic/glycolytic pathways in the liver and fat mobilization in white adipocytes in response to fasting (7,8). SirT1 deacetylase activity is inhibited by nicotinamide and activated by resveratrol. In addition, SirT1 activity may be regulated by phosphorylation, as it is phosphorylated at Ser27 and Ser47 in vivo; however, the function of these phosphorylation sites has not yet been determined (9).

沉默信息调节因子(SIR2)基因家族是一类高度保守的基因,能够编码烟酰胺腺嘌呤二核苷酸(NAD)依赖性的去乙酰化酶,亦称III类组蛋白去乙酰化酶。在这些基因中,酿酒酵母Sir2是最早被发现,并且了解得最清楚的。Sir2 参与了交配型基因座的沉默,端粒维持,DNA损伤应答和细胞衰老(1)。SirT1, 是哺乳动物中Sir2的直系同源基因,是一个核蛋白,与许多细胞过程调控相关联,包括细胞凋亡、细胞衰老、内分泌信号转导、糖稳态、衰老和长寿。SirT1的靶蛋白包括乙酰化的p53 (2,3)、 p300 (4)、 Ku70 (5)、 forkhead (FoxO) 转录因子(5,6)、 PPARγ (7)和PPARγ 共活化因子-1α (PGC-1α)蛋白(8)。p53和FoxO转录因子的去乙酰化会能够足额凋亡并且提高细胞存活率(2,3,5,6)。PPARγ和PGC-1α的去乙酰化能够调控肝脏中糖异生/糖酵解通路以及白色脂肪细胞中的脂肪动员来对禁食做出的应答(7,8)。SirT1去乙酰化酶活性可以被尼克酰胺抑制,而被白藜三醇活化。由烟碱禁止并且resveratrol激活deacetylase活动。另外SirT1活性可能是通过磷酸化作用来调控。因为在机内它的Ser27和Ser47 位点是磷酸化的,但是这些磷酸化位点的具体功能尚不明确(9)。

  1. Guarente, L. (1999) Nat. Genet. 23, 281-285.
  2. Vaziri, H. et al. (2001) Cell 107, 149-159.
  3. Luo, J. et al. (2001) Cell 107, 137-148.
  4. Bouras, T. et al. (2005) J. Biol. Chem. 280, 10264-10276.
  5. Brunet, A. et al. (2004) Science 303, 2011-2015.
  6. Motta, M.C. et al. (2004) Cell 116, 551-563.
  7. Picard, F. et al. (2004) Nature 429, 771-776.
  8. Rodgers, J.T. et al. (2005) Nature 434, 113-118.
  9. Beausoleil, S.A. et al. (2004) Proc. Natl. Acad. Sci. USA 101, 12130-12135.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

SignalSilence is a registered trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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