Cell Signaling Technology

Product Pathways - Ca / cAMP / Lipid Signaling

Calreticulin (D3E6) XP® Rabbit mAb #12238

Calreticulin   sc-166837   sc-6467  

No. Size Price
12238S 100 µl ( 10 western blots ) ¥3,750.00 现货查询 购买询价 防伪查询
12238T 20 µl ( 2 western blots ) ¥1,400.00 现货查询 购买询价 防伪查询
12238 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat, Endogenous 55 Rabbit IgG
IF-IC 1:400

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IF-IC=Immunofluorescence (Immunocytochemistry),

Specificity / Sensitivity

Calreticulin (D3E6) XP® Rabbit mAb recognizes endogenous levels of total calreticulin protein.

Calreticulin (D3E6) XP® Rabbit mAb兔单抗识别内源性的总钙网蛋白。

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human calreticulin protein.




Confocal immunofluorescent analysis of NIH/3T3 cells using Calreticulin (D3E6) XP® Rabbit mAb (green) and β-Actin (13E5) Rabbit mAb (Alexa Fluor® 555 Conjugate) #8046 (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

激光共聚焦荧光法检测NIH/3T3 细胞,使用的抗体为Calreticulin (D3E6) XP® Rabbit mAb (绿色) 和 β-Actin (13E5) Rabbit mAb (Alexa Fluor® 555 Conjugate) #8046 (红色).蓝色伪彩色为DNA荧光染料(产品信息为DRAQ5® #4084)。

Western Blotting

Western Blotting

Western blot analysis of extracts from SH-SY5Y and KNRK cells using Calreticulin (D3E6) XP® Rabbit mAb.

Western blot方法检测SH-SY5Y 和KNRK细胞提取物,使用的抗体为Calreticulin (D3E6) XP® Rabbit mAb.


Calcium is a universal signaling molecule involved in many cellular functions such as cell motility, metabolism, protein modification, protein folding, and apoptosis. Calcium is stored in the endoplasmic reticulum (ER), where it is buffered by calcium binding chaperones such as calnexin and calreticulin, and is released via the IP3 Receptor channel (1). Calreticulin also functions as an ER chaperone that ensures proper folding and quality control of newly synthesized glycoproteins. As such, calreticulin presumably does not alter protein folding but regulates proper timing for efficient folding and subunit assembly. Furthermore, calreticulin retains proteins in non-native conformation within the ER and targets them for degradation (2,3).


  1. Groenendyk, J. et al. (2004) Mol Cells 17, 383-9.
  2. Nauseef, W.M. et al. (1995) J Biol Chem 270, 4741-7.
  3. Williams, D.B. (2006) J Cell Sci 119, 615-23.

Application References

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!


Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

DRAQ5 is a registered trademark of Biostatus Limited.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

XP is a registered trademark of Cell Signaling Technology, Inc.

Alexa Fluor is a registered trademark of Life Technologies Corporation.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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