Cell Signaling Technology

Product Pathways - Ca / cAMP / Lipid Signaling

Calreticulin (D3E6) XP® Rabbit mAb #12238

Calreticulin   sc-166837   sc-6467  

No. Size Price
12238S 100 µl ( 10 western blots ) ¥3,580.00 现货查询 购买询价
12238T 20 µl ( 2 western blots ) ¥1,400.00 现货查询 购买询价
12238 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat, Endogenous 55 Rabbit IgG
IF-IC 1:400

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IF-IC=Immunofluorescence (Immunocytochemistry),

Specificity / Sensitivity

Calreticulin (D3E6) XP® Rabbit mAb recognizes endogenous levels of total calreticulin protein.

Calreticulin (D3E6) XP® Rabbit mAb兔单抗识别内源性的总钙网蛋白。

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human calreticulin protein.

该单克隆抗体通过用合成肽免疫动物制备,该合成肽与人钙网蛋白靠近氨基末端的残基一样。

IF-IC

IF-IC

Confocal immunofluorescent analysis of NIH/3T3 cells using Calreticulin (D3E6) XP® Rabbit mAb (green) and β-Actin (13E5) Rabbit mAb (Alexa Fluor® 555 Conjugate) #8046 (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

激光共聚焦荧光法检测NIH/3T3 细胞,使用的抗体为Calreticulin (D3E6) XP® Rabbit mAb (绿色) 和 β-Actin (13E5) Rabbit mAb (Alexa Fluor® 555 Conjugate) #8046 (红色).蓝色伪彩色为DNA荧光染料(产品信息为DRAQ5® #4084)。

Western Blotting

Western Blotting

Western blot analysis of extracts from SH-SY5Y and KNRK cells using Calreticulin (D3E6) XP® Rabbit mAb.

Western blot方法检测SH-SY5Y 和KNRK细胞提取物,使用的抗体为Calreticulin (D3E6) XP® Rabbit mAb.

Background

Calcium is a universal signaling molecule involved in many cellular functions such as cell motility, metabolism, protein modification, protein folding, and apoptosis. Calcium is stored in the endoplasmic reticulum (ER), where it is buffered by calcium binding chaperones such as calnexin and calreticulin, and is released via the IP3 Receptor channel (1). Calreticulin also functions as an ER chaperone that ensures proper folding and quality control of newly synthesized glycoproteins. As such, calreticulin presumably does not alter protein folding but regulates proper timing for efficient folding and subunit assembly. Furthermore, calreticulin retains proteins in non-native conformation within the ER and targets them for degradation (2,3).

钙是一种通用的信号传导分子,参与许多细胞功能,如细胞运动、代谢、蛋白质修饰、蛋白质折叠和细胞凋亡。钙存储于内质网(ER),在内质网内与钙离子结合分子伴侣如钙联接蛋白和钙网蛋白缓冲,并通过IP3受体通道释放(1)。钙网蛋白也作为内质网的分子伴侣起作用,以确保新合成糖蛋白的正确折叠和质量控制。因此,推测钙网蛋白不会改变蛋白质的折叠,而是在恰当的时间点调节有效折叠和亚基组装。此外,在内质网中,钙网蛋白以非天然构象保存,并对其进行靶向降解(2,3)。

  1. Groenendyk, J. et al. (2004) Mol Cells 17, 383-9.
  2. Nauseef, W.M. et al. (1995) J Biol Chem 270, 4741-7.
  3. Williams, D.B. (2006) J Cell Sci 119, 615-23.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

DRAQ5 is a registered trademark of Biostatus Limited.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

XP is a registered trademark of Cell Signaling Technology, Inc.

Alexa Fluor is a registered trademark of Life Technologies Corporation.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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