Cell Signaling Technology

Product Pathways - Adhesion

Syndecan 4 Antibody #12236

No. Size Price
12236S 100 µl ( 10 western blots ) ¥3,100.00 现货查询 购买询价
12236 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat,Monkey, Endogenous 29 Rabbit

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting,

Specificity / Sensitivity

Syndecan 4 Antibody recognizes endogenous levels of total Syndecan 4 protein.

Syndecan 4 Antibody用于检测内源性的总Syndecan 4蛋白。

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro103 of human Syndecan 4 protein. Antibodies are purified by protein A and peptide affinity chromatography.

该多克隆抗体由人Syndecan 4蛋白Pro103附近的残基合成多肽免疫动物产生。该抗体由protein A和肽亲和层析纯化。

Western Blotting

Western Blotting

Western blot analysis of extracts from Jurkat and HaCaT cells using Syndecan 4 Antibody.Western blot检测 Jurkat和HaCaT细胞提取物。


Syndecans are a family of type 1 transmembrane heparan sulphate proteoglycans comprising 4 members in mammals (SDC-1 to -4) (1) encoded by four syndecan genes. Syndecans are involved in embryonic development, tumorigenesis, and angiogenesis (2). The extracellular domain harbors attachment sites for heparan sulfate and chondroitin sulfate chains, facilitating interaction with an array of proteins including a plethora of growth factors. In addition, the hydrophobic C-terminal intracellular domain can interact with proteins containing a PDZ domain (2). These interactions place syndecans as important integrators of membrane signaling (3). Syndecans undergo proteolytic cleavage causing the release of their extracellular domain (shedding), converting the membrane-bound proteins into soluble molecular effectors (4).

Syndecans是哺乳动物跨膜硫酸乙酰肝素蛋白聚糖组成4 (SDC-1 to -4) (1)家族成员,通过4个多配体蛋白聚糖基因编码。多配体蛋白聚糖在胚胎发育,肿瘤生成和血管发生中起作用(2)。细胞外结构域包含了硫酸乙酰肝素和硫酸软骨素链的粘附位点,促进了细胞间蛋白排列的相互作用,包括大量的生长因子。此外,疏水的C-末端细胞内结构域能够与包含PDZ结构域的蛋白相互作用(2)。这些syndecans的相互作用位点,对于膜信号转导起重要整合作用(3)。Syndecans溶蛋白性水解后,引起了细胞外结构域的释放(脱落),产生膜结合蛋白到可溶性分子效应物的转化(4)。

Syndecan 4 is expressed ubiquitously and is enriched in the focal adhesions of various adherent cell types (5). Syndecan 4 controls focal adhesion formation and regulates cell migration through interaction with Rho family GTPase and protein kinase C family members (6-11). Phosphorylation of the cytoplasmic domain is linked to ectodomain shedding of Syndecan 4, both of which contribute to the regulation of cytokinesis (12).

Syndecan 4广泛表达,并在许多粘附细胞类型中大量存在于黏着斑(5)。Syndecan 4控制黏着斑的形成并通过与Rho家族GTPase和蛋白激酶C家族成员的相互作用调节细胞迁移(6-11)。胞浆区结构域的磷酸化与Syndecan 4外功能区的脱落有关,两者均对细胞因子的调节起作用(12)。

  1. Couchman, J.R. (2003) Nat Rev Mol Cell Biol 4, 926-37.
  2. Multhaupt, H.A. et al. (2009) J Physiol Pharmacol 60 Suppl 4, 31-8.
  3. Zimmermann, P. and David, G. (1999) FASEB J 13 Suppl, S91-S100.
  4. Manon-Jensen, T. et al. (2010) FEBS J 277, 3876-89.
  5. Woods, A. and Couchman, J.R. (1994) Mol Biol Cell 5, 183-92.
  6. Harrington, E.O. et al. (1997) J Biol Chem 272, 7390-7.
  7. Oh, E.S. et al. (1997) J Biol Chem 272, 8133-6.
  8. Woods, A. and Couchman, J.R. (1992) J Cell Sci 101 ( Pt 2), 277-90.
  9. Longley, R.L. et al. (1999) J Cell Sci 112 ( Pt 20), 3421-31.
  10. Woods, A. et al. (2000) Arch Biochem Biophys 374, 66-72.
  11. Bishop, A.L. and Hall, A. (2000) Biochem J 348 Pt 2, 241-55.
  12. Keller-Pinter, A. et al. (2010) Cell Mol Life Sci 67, 1881-94.

Application References

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!


Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

用户评论 --- 共 0


我要参与评论 :