Cell Signaling Technology

Product Pathways - Neuroscience

Tenascin C (D16C4) Rabbit mAb #12221

No. Size Price
12221S 100 µl ( 10 western blots ) ¥3,100.00 现货查询 购买询价
12221 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat, Endogenous 240 Rabbit IgG
IP 1:50

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation,

Specificity / Sensitivity

Tenascin C (D16C4) Rabbit mAb recognizes endogenous levels of total Tenascin C protein. This antibody also cross-reacts with a protein of unknown origin at 120 kDa.

Tenascin C (D16C4) Rabbit mAb能够检测内源性的肌腱蛋白C总蛋白。该抗体可能会与未知来源的120 kDa蛋白产生交叉反应。

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human Tenascin C protein.

该单克隆抗体是由合成的人源肌腱蛋白C蛋白C末端肽段免疫动物而制备的。

Western Blotting

Western Blotting

Western blot analysis of extracts from mouse brain and rat brain using Tenascin C (D16C4) Rabbit mAb.

Western blot 检测小鼠大脑和大鼠大脑提取物,使用的抗体是Tenascin C (D16C4) Rabbit mAb兔单抗。

Background

Tenascin C is a large hexameric extracellular matrix glycoprotein that exhibits de-adhesive effects on cell-matrix interaction, enhancing cell proliferation and motility in most cell types. It is highly expressed in remodeling tissues during embryonic development and under pathological conditions in adults, and research studies have shown markedly increased expression in cancerous tissues (1,2). Tenascin C has been implicated in a variety of cellular processes relevant to atherosclerosis, including cell proliferation, migration, and apoptosis. Expression of Tenascin C is tightly controlled in adults and is upregulated in tissues undergoing wound healing (3). In development, the expression of Tenascin C is known to be associated with epithelial-mesenchymal transition (EMT) events including gastrulation and formation of the neural crest, endocardial cushion, and secondary palate (1). Investigators have shown that Tenascin C is a key determinant of the tumor stroma and that it is involved in the initiation of tumorigenesis and progression to metastasis (2). Immature and mature astrocytes, radial glial cells, Schwann cells, and a subset of neurons express Tenascin C. Upon CNS trauma or exposure of neurons to excitotoxic agents, Tenascin C expression is upregulated by glial cells. Research studies have shown that Tenascin C is involved in guidance of migrating axons and neurons, synaptic plasticity, and neuronal regeneration, promoting spinal cord regeneration after injury (4).

肌腱蛋白C是一个大的六聚体细胞外基质糖蛋白,在大多数类型的细胞中表现出去粘着的功能,影响细胞-基质相互作用,提高细胞增殖和活力。它在胚胎发育和成人的病理条件下高表达于重塑组织中,研究显示其在癌组织中的表达量显着增加(1,2)。肌腱蛋白C与各种动脉粥样硬化相关的细胞过程有密切关系,包括细胞增殖,迁移,凋亡。在成人中,肌腱蛋白C的表达受严格控制,并在伤口愈合组织上调表达(3)。在发育过程中,已知肌腱蛋白C的表达是与上皮-间质转化(EMT)事件相关,包括原肠胚形成和神经嵴,心内膜垫和次生腭的形成(1)。调查表明,肌腱蛋白C是肿瘤基质的一个关键因素,它参与着肿瘤从初始发生,发展到转移(2)。不成熟和成熟的星形胶质细胞,放射状胶质细胞,雪旺氏细胞和一个子神经元表达肌腱蛋白C。中枢神经系统损伤或神经元暴露于兴奋性药物时,肌腱蛋白C会被神经胶质细胞上调表达。研究表明,肌腱蛋白C的参与调控轴突和神经元的迁移,神经元突触可塑性,神经细胞再生以及促进脊髓损伤后再生(4)。

  1. Imanaka-Yoshida, K. (2012) Circ J 76, 2513-20.
  2. Yoshimura, H. et al. (2011) Histol Histopathol 26, 297-305.
  3. Minear, M.A. et al. (2011) Hum Genet 129, 641-54.
  4. Chen, J. et al. (2010) Mol Ther 18, 1769-77.

Application References

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Protocols


For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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