Product Pathways - Cell Cycle / Checkpoint
Cyclin D1 (92G2) Rabbit mAb (PE Conjugate) #12205
|12205S||100 µl ( 50 tests )||￥3,986.00 现货查询||购买询价|
|12205||carrier free & custom formulation / quantity||email request|
Species cross-reactivity is determined by western blot.
Applications Key: F=Flow Cytometry,
Specificity / Sensitivity
Cyclin D1 (92G2) Rabbit mAb (PE Conjugate) detects endogenous levels of total cyclin D1 protein.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to the carboxy-terminus of human cyclin D1.
This Cell Signaling Technology antibody is conjugated to phycoerythrin (PE) and tested in-house for direct flow cytometry analysis in human cells. The antibody is expected to exhibit the same species cross-reactivity as the unconjugated Cyclin D1 (92G2) Rabbit mAb #2978.
Activity of the cyclin-dependent kinases CDK4 and CDK6 is regulated by T-loop phosphorylation, by the abundance of their cyclin partners (the D-type cyclins), and by association with CDK inhibitors of the Cip/Kip or INK family of proteins (1). The inactive ternary complex of cyclin D/CDK4 and p27 Kip1 requires extracellular mitogenic stimuli for the release and degradation of p27 concomitant with a rise in cyclin D levels to affect progression through the restriction point and Rb-dependent entry into S-phase (2). The active complex of cyclin D/CDK4 targets the retinoblastoma protein for phosphorylation, allowing the release of E2F transcription factors that activate G1/S-phase gene expression (3). Levels of cyclin D protein drop upon withdrawal of growth factors through downregulation of protein expression and phosphorylation-dependent degradation (4).
- Hirai, H. et al. (1995) Mol Cell Biol 15, 2672-81.
- Sherr, C.J. (1996) Science 274, 1672-7.
- Lukas, J. et al. (1996) Mol Cell Biol 16, 6917-25.
- Diehl, J.A. et al. (1997) Genes Dev 11, 957-72.
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
- 2978 Cyclin D1 (92G2) Rabbit mAb
- 8497 Phospho-Cyclin D1 (Thr286) (D29B3) XP® Rabbit mAb (PE Conjugate)
For Research Use Only. Not For Use In Diagnostic Procedures.
U.S. Patent No. 5,675,063.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
XP is a registered trademark of Cell Signaling Technology, Inc.
Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.
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