Cell Signaling Technology

Product Pathways - Lymphocyte Signaling

CCR2 (D14H7) Rabbit mAb #12199

No. Size Price
12199S 100 µl ( 10 western blots ) ¥3,100.00 现货查询 购买询价
12199 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human, Endogenous 42 Rabbit IgG
IP 1:100

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation,

Specificity / Sensitivity

CCR2 (D14H7) Rabbit mAb recognizes endogenous levels of total CCR2 protein.

CCR2 (D14H7) Rabbit mAb抗体能识别内源性CCR2总蛋白水平。

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly197 of human CCR2 protein.

此单克隆抗体通过用合成肽免疫动物制备,该合成肽是人CCR2蛋白Gly197周围的残基。

IP

IP

Immunoprecipitation of CCR2 from THP-1 cell extracts using Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) or CCR2 (D14H7) Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using CCR2 (D14H7) Rabbit mAb.免疫沉淀分析来源于THP-1细胞提取物的CCR2。使用的抗体是:Rabbit (DA1E) mAb IgG XP® Isotype Control #3900(泳道2)或CCR2 (D14H7) Rabbit mAb(泳道3)。泳道1是10% input。Western blot分析使用的抗体是CCR2 (D14H7) Rabbit mAb。

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using CCR2 (D14H7) Rabbit mAb (upper) or GAPDH (D16H11) XP® Rabbit mAb #5174 (lower). HEL cells are reported to be negative for CCR2 expression (2).Western blot分析来自于不同细胞系的提取物。使用的抗体是:CCR2 (D14H7) Rabbit mAb(上图)、GAPDH (D16H11) XP® Rabbit mAb #5174(下图)。HEL细胞不表达CCR2(2)。

Background

CCR2 is a member of the “CC-branch” of chemokine G protein-coupled receptors that regulate monocyte chemotaxis and T cell migration/activation and drive inflammation in a number of pathological conditions (1). CCR2 is the receptor for several chemokines including MCP-1, MCP-3, and MCP-4 (2-5). CCR2 transduces signals through increases in intracellular calcium levels. It has two alternative isoforms, CCR2A and CCR2B, differing in their carboxy-terminal tails with CCR2B trafficking more efficiently to the membrane (2,6). CCR2 was originally identified in the THP-1 monocyte cell line, and its expression is decreased following differentiation into macrophages (7). Knockout studies demonstrate that CCR2 is a major regulator of macrophage trafficking (8-10). In addition, research studies have shown that CCR2 functions as an alternative coreceptor with CD4 for infection of some strains of HIV (11,12).

CCR2是趋化因子G蛋白偶联受体的“CC分支”中的成员,能够调节单核细胞趋化性和T细胞迁移/活化以及在许多病理条件下能促进炎症反应(1)。CCR2是多种趋化因子的受体,包括MCP-1,MCP-3和MCP-4 (2-5)。CCR2能通过提高细胞内钙水平来进行信号转导。它有两个亚型,分别为CCR2A和CCR2B,两者羧基末端的尾巴有差异,CCR2B能够更有效地运输到膜(2,6)。CCR2最初是在THP-1单核细胞系中发现的,它的表达随着分化成巨噬细胞而减少(7)。敲除试验显示CCR2是巨噬细胞运输的主要“调节器”(8-10)。另外,研究表明,在一些HIV的感染中,CCR2与CD4是共同受体(11,12)。

  1. Zhao, Q. (2010) J Leukoc Biol 88, 41-55.
  2. Charo, I.F. et al. (1994) Proc Natl Acad Sci U S A 91, 2752-6.
  3. Franci, C. et al. (1995) J Immunol 154, 6511-7.
  4. Yamagami, S. et al. (1994) Biochem Biophys Res Commun 202, 1156-62.
  5. Combadiere, C. et al. (1995) J Biol Chem 270, 29671-5.
  6. Wong, L.M. et al. (1997) J Biol Chem 272, 1038-45.
  7. Denholm, E.M. and Stankus, G.P. (1995) Cytokine 7, 436-40.
  8. Kuziel, W.A. et al. (1997) Proc Natl Acad Sci U S A 94, 12053-8.
  9. Boring, L. et al. (1997) J Clin Invest 100, 2552-61.
  10. Kurihara, T. et al. (1997) J Exp Med 186, 1757-62.
  11. Rucker, J. et al. (1996) Cell 87, 437-46.
  12. Frade, J.M. et al. (1997) J Clin Invest 100, 497-502.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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