Cell Signaling Technology

Product Pathways - Development

KLF4 (D1F2) Rabbit mAb #12173

sc-20691   sc-393462  

No. Size Price
12173S 100 µl ( 10 western blots ) ¥3,100.00 现货查询 购买询价
12173 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human, Endogenous 62 Rabbit IgG
IP 1:100

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation,

Homology

Species predicted to react based on 100% sequence homology: Monkey,

Specificity / Sensitivity

KLF4 (D1F2) Rabbit mAb recognizes endogenous levels of total KLF4 protein.

KLF4 (D1F2)Rabbit mAb兔单抗可以识别内源性的总KLF4蛋白。

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human KLF protein.

单克隆抗体由合成肽段免疫动物产生,该肽段与人KLF的羧基末端邻近氨基酸残基序列对应。

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using KLF4 (D1F2) Rabbit mAb.

使用KLF4 (D1F2)Rabbit mAb兔单抗对多种细胞提取物进行western blot分析。

Background

KLF4 is a member of the erythroid Kruppel-like factor (EKLF) multigene family that is highly expressed in the differentiating layers of the epidermis (1, 2). KLF4 plays a critical role in the differentiation of epithelial cells and is essential for normal gastric homeostasis (2,3). Depending on the target gene, KLF4 can function as both a repressor and activator of transcription (4). Research studies suggest this protein may function as either a tumor suppressor or an oncogene depending on tumor type, with up-regulation in human squamous cell carcinoma of the head and neck and down-regulation in colorectal carcinoma (5,6). The in vitro reprogramming of somatic cells to an embryonic-like state has been achieved by retroviral transduction of four factors: Oct-3/4, Sox2, c-Myc, and KLF4 (7). These induced pluripotent stem cells (iPS) are of great therapeutic interest as they exhibit the key characteristics and growth properties of pluripotent stem cells (8,9).

KLF4是erythroid Kruppel-like factor (EKLF)多基因家族一员,在分化的表皮层中高表达(1,2)。KLF4在表皮细胞分化过程中发挥重要功能,对于正常胃稳态是必须的(2,3)。KLF4既可以作为转录抑制子也可以作为激活子,这取决于它的靶基因(4)。研究提示该蛋白的即可作为抑癌也可以作为促癌基因,这取决于肿瘤的类型,它在人头部和脖子的扁平上皮癌中表达上调,在结肠癌中低表达(5,6)。体细胞的体外重编程为胚胎样细胞是通过四个逆转录因子:Oct-3/4, Sox2, c-Myc, 和 KLF4实现的 (7)。诱导的多潜能干细胞(iPS)是研究热点,因为他们表现了多潜能干细胞的重要特征和生长性能(8,9).

  1. Yet, S.F. et al. (1998) J Biol Chem 273, 1026-31.
  2. Segre, J.A. et al. (1999) Nat Genet 22, 356-60.
  3. Katz, J.P. et al. (2005) Gastroenterology 128, 935-45.
  4. Evans, P.M. and Liu, C. (2008) Acta Biochim Biophys Sin (Shanghai) 40, 554-64.
  5. Foster, K.W. et al. (2005) Oncogene 24, 1491-500.
  6. Rowland, B.D. and Peeper, D.S. (2006) Nat Rev Cancer 6, 11-23.
  7. Takahashi, K. and Yamanaka, S. (2006) Cell 126, 663-76.
  8. Meissner, A. et al. (2007) Nat Biotechnol 25, 1177-81.
  9. Park, I.H. et al. (2008) Nature 451, 141-6.

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For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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