Cell Signaling Technology

Product Pathways - Lymphocyte Signaling

HS1 (D83A8) XP® Rabbit mAb (Human Specific) (Alexa Fluor® 647 Conjugate) #12162

No. Size Price
12162S 100 µl ( 50 tests ) ¥4,264.00 现货查询 购买询价
12162 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
F 1:50 Human, Endogenous Rabbit IgG
IF-IC 1:50

Species cross-reactivity is determined by western blot.

Applications Key: F=Flow Cytometry, IF-IC=Immunofluorescence (Immunocytochemistry),

Specificity / Sensitivity

HS1 (D83A8) XP® Rabbit mAb (Human Specific) (Alexa Fluor® 647 Conjugate) recognizes endogenous levels of total HS1 protein.

HS1 (D83A8) XP® Rabbit mAb (人特异性) (Alexa Fluor® 647偶联)检测内源性HS1总蛋白水平。

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro310 of human HS1 protein.

此单克隆抗体通过合成肽免疫动物制备,这种合成肽为人源HS1蛋白周围的肽段。

Description

This Cell Signaling Technology antibody is conjugated to Alexa Fluor® 647 fluorescent dye and tested in-house for direct immunofluorescence and flow cytometric analysis in human cells. This antibody is expected to exhibit the same species cross-reactivity as the unconjugated HS1 (D83A8) XP® Rabbit mAb (Human Specific) #3890.

此抗体结合Alexa Fluor® 647荧光染料,并且利用对人源细胞的直接免疫荧光和流式细胞术分析来进行严格的内部检测。这种抗体对相同来源的如未偶联HS1 (D83A8) XP® Rabbit mAb (Human Specific) #3890具有相同交叉反应活性。

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of MCF7 (blue) and Jurkat (green) cells using HS1 (D83A8) XP® Rabbit mAb (Human Specific) (Alexa Fluor® 647 Conjugate). 对MCF7细胞(蓝色)和Jurkat细胞(绿色)进行流式细胞术分析,使用抗体是 HS1 (D83A8) XP® Rabbit mAb (Human Specific) (Alexa Fluor® 647 Conjugate)。

IF-IC

IF-IC

Confocal immunofluorescent analysis of Jurkat (left) and 293 (right) cells using HS1 (D83A8) XP® Rabbit mAb (Human Specific) (Alexa Fluor® 647 Conjugate) (red). Blue pseudocolor = Propidium Iodide (PI)/RNase Staining Solution #4087. 共聚焦分析Jurkat(左图)和293细胞(右图),使用抗体是HS1 (D83A8) XP® Rabbit mAb (Human Specific) (Alexa Fluor® 647 Conjugate) (红色). Blue pseudocolor = Propidium Iodide (PI)/RNase Staining Solution #4087.

Background

HS1 (HCLS1, LckBP1, p75) is a protein kinase substrate that is expressed only in tissues and cells of hematopoietic origin (1,2). HS1 contains four cortactin repeats and a single SH3 domain (2). This intracellular protein is phosphorylated following immune receptor activation, which promotes recruitment of HS1 to the immune synapse (3-5). Phosphorylation of HS1 is required to regulate actin dynamics and provide docking sites for many other signaling molecules, such as Vav1 and PLCγ1 (6). HS1 also plays an important role in platelet activation (7).

HS1 (HCLS1, LckBP1, p75) 是蛋白激酶底物,只在造血组织和细胞中表达(1,2)。HS1包含四个皮肌动蛋白重复和单个SH3域(2)。这种胞内蛋白在免疫受体的激活下发生磷酸化, 从而促进HS1被招募到免疫突触处(3-5)。磷酸化的HS1被用于调节肌动蛋白的动态并为其它信号分子如Vav1 和 PLCγ1提供对接位点(6)。HS1在血小板活化过程中也发挥了重要的作用(7)。

  1. Kitamura, D. et al. (1989) Nucleic Acids Res 17, 9367-79.
  2. Kitamura, D. et al. (1995) Biochem Biophys Res Commun 208, 1137-46.
  3. Suzuki, H. et al. (1997) J Immunol 159, 5881-8.
  4. Hata, D. et al. (1994) Immunol Lett 40, 65-71.
  5. Yamanashi, Y. et al. (1993) Proc Natl Acad Sci USA 90, 3631-5.
  6. Gomez, T.S. et al. (2006) Immunity 24, 741-52.
  7. Kahner, B.N. et al. (2007) Blood 110, 2449-56.

Application References

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Protocols

Companion Products


For Research Use Only. Not For Use In Diagnostic Procedures.

The Alexa Fluor dye antibody conjugates in this product are sold under license from Life Technologies Corporation for research use only, except for use in combination with DNA microarrays. The Alexa Fluor® dyes (except for Alexa Fluor® 430 dye) are covered by pending and issued patents. Alexa Fluor® is a registered trademark of Molecular Probes, Inc.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

XP is a registered trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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