Cell Signaling Technology

Product Pathways - Cell Cycle / Checkpoint

Ki-67 (D3B5) Rabbit mAb (PE Conjugate) #12160

MIB1   SP6  

No. Size Price
12160S 100 µl ( 50 tests ) ¥3,986.00 现货查询 购买询价
12160 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
F 1:50 Human,Mouse,Rat, Endogenous Rabbit IgG

Species cross-reactivity is determined by western blot.

Applications Key: F=Flow Cytometry,

Specificity / Sensitivity

Ki-67 (D3B5) Rabbit mAb (PE Conjugate) recognizes endogenous levels of total Ki-67 protein.Ki-67 (D3B5)Rabbit mAb (PE 偶联)可以识别内源性Ki-67总蛋白。

Source / Purification

Monoclonal antibody is produced by immunizing animals with a recombinant protein specific to the amino terminus of human Ki-67 protein. 单克隆抗体由合成肽段免疫动物产生,该肽段与人Ki-67蛋白的氨基端邻近残基序列一致。

Description

This Cell Signaling Technology antibody is conjugated to phycoerythrin (PE) and tested in-house for direct flow cytometry analysis in human cells. The antibody is expected to exhibit the same species cross-reactivity as the unconjugated Ki-67 (D3B5) Rabbit mAb #9129.Cell Signaling Technology公司抗体偶联藻红蛋白(PE)并经人类细胞直接流式细胞仪免疫荧光分析内部测试。抗体预期和未偶联的Ki-67 (D3B5)Rabbit mAb #9129显示同样的物种交叉反应。

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of Jurkat cells using Ki-67 (D3B5) Rabbit mAb (PE Conjugate) and DRAQ5® #4084 (fluorescent DNA dye).使用Ki-67 (D3B5)Rabbit mAb (PE 偶联)和DRAQ5® #4084 (fluorescent DNA dye)对Jurkat细胞进行流式分析。

Background

Ki-67, named after the location where it was discovered (Kiel University, Germany), is a nuclear nonhistone protein (1) that is universally expressed among proliferating cells and absent in quiescent cells (2). Ki-67 detects proliferating cells in G1, S, G2, and mitosis, but not in the G0 resting phase. Research studies have shown that high levels of Ki-67 are associated with poorer breast cancer survival (3). Research studies have explored the use of Ki-67, along with other markers, as potential prognostic or predictive markers in breast cancer and other malignant diseases (4).Ki-67,以其发现地命名(Kiel大学,德国),作为一个细胞核非组蛋白(1)通常在增殖的细胞中表达并且在休眠细胞中不表达(2)。Ki-67会让增殖细胞停滞在G1,S,G2和有丝分裂期,但是不会停滞在G0期。研究显示高表达的Ki-67与低乳腺癌存活率相关(3)。研究已经将Ki-67与其他的标记,作为乳腺癌和其他恶性疾病中的潜在的预后和预测标记(4)。

  1. Gerdes, J. et al. (1983) Int J Cancer 31, 13-20.
  2. Weigel, M.T. and Dowsett, M. (2010) Endocr Relat Cancer 17, R245-62.
  3. Jones, R.L. et al. (2009) Breast Cancer Res Treat 116, 53-68.
  4. Yerushalmi, R. et al. (2010) Lancet Oncol 11, 174-83.

Application References

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!

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For Research Use Only. Not For Use In Diagnostic Procedures.

DRAQ5 is a registered trademark of Biostatus Limited.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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