Product Pathways - Tyrosine Kinase / Adaptors
Human EGF Neutralizing (D8A1) Rabbit mAb #12157
|12157S||100 µg||￥4,264.00 现货查询||购买询价|
|12157||carrier free & custom formulation / quantity||email request|
Species cross-reactivity is determined by western blot.
Applications Key: N=Neutralizing,
Specificity / Sensitivity
Human EGF Neutralizing (D8A1) Rabbit mAb binds to human EGF and neutralizes its effects in an MCF 10A cell proliferation assay. This antibody does not cross-react with human betacellulin or human TGF-α.
人EGF Neutralizing (D8A1) Rabbit mAb（中和兔单抗）能够与人源EGF相结合，并且能够在10A细胞增殖测试实验发挥中和EGF活性的作用。这种抗体不会与人源细胞调节素或者人源TGF-α发生交叉反应。
Source / Purification
Monoclonal antibody is produced by immunizing animals with a recombinant human EGF protein.
Neutralizing antibodies can be used to inhibit normal biological function through their binding to biological molecules. These reagents can be used to determine the effects that a particular molecule has in biological systems. Human EGF Neutralizing (D8A1) Rabbit mAb has been shown to neutralize the EGF-induced proliferation of MCF 10A cells in vitro with an ND50 in the range of 200-700 ng/ml.
中和抗体能够通过结合生物分子抑制正常的生物功能。这些试剂可以用来确定一个特定的大分子在生物系统中的影响。人EGF Neutralizing (D8A1) Rabbit mAb（中和兔单抗）能够中和体外EGF诱导的MCF 10A细胞的增殖，ND50的范围在200-700 ng/ml。
The proliferation of MCF 10A cells treated with increasing concentrations of hEGF #8916 was assessed. After 24 hr, cells were labeled with BrdU for 4 hr and BrdU incorporation was determined using BrdU Cell Proliferation Assay Kit #6813.MCF 10A细胞的增殖通过递增浓度的hEGF #8916处理进行评估。24小时后，用BrdU标记细胞4小时，BrdU的结合用 BrdU Cell Proliferation Assay Kit #6813测定。
The ability of Human EGF Neutralizing (D8A1) Rabbit mAb to inhibit hEGF-induced MCF 10A cell proliferation was assessed. MCF 10A cells were treated with increasing concentrations of antibody in the presence of hEGF #8916 (2 ng/ml). After 24 hr, cells were labeled with BrdU for 4 hr and BrdU incorporation was determined using BrdU Cell Proliferation Assay Kit #6813.Human EGF Neutralizing (D8A1) Rabbit mAb能够抑制hEGF诱导的MCF 10A细胞的增殖。MCF 10A细胞通过hEGF #8916 (2 ng/ml)浓度抗体处理。24小时后，细胞被用BrdU标记4小时，BrdU的结合用 BrdU Cell Proliferation Assay Kit #6813测定。
EGF is produced by epithelial cells, fibroblasts, and many other cell types (1,2). Low molecular weight soluble EGF is generated through proteolysis of a larger ~130,000 kDa transmembrane precursor (1,2). Both soluble and membrane forms of EGF are active (2). EGF induces proliferation, differentiation, and survival of many cell types including tumor-derived cells (1-3). There are multiple members of the EGF family and multiple members of the HER/ErbB EGF receptor family. EGF binds to HER1/ErbB1 and induces homo- or heterodimerization with other HER/ErbB family members, resulting in signaling through the MAPK, PI3K/Akt, and Stat5 pathways (1). Research studies have implicated EGF, EGF family members, EGF receptors, and their signaling pathways in many cancers (1,2).
EGF是由上皮细胞、成纤维细胞以及很多其它细胞型产生的(1,2)。低分子量可溶性的EGF通过一个横跨膜前导蛋白的蛋白质水解产生(1,2)。可溶性的和细胞膜形式的EGF都是有活性的(2)。EGF诱导许多细胞类型，包括肿瘤衍生细胞的增殖、分化和存活(1-3)。EGF家族和HER/ErbB EGF受体家族都有很多成员。EGF能够与HER1/ErbB1结合并且诱导与HER1/ErbB1家族的同源二聚体和异源二聚体的形成，导致MAPK、PI3K/Akt和Stat5 信号通路的激活(1)。在很多癌症中，EGF、EGF家族成员、EGF受体以及它们的通路都相关(1,2)。
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For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.
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