Cell Signaling Technology

Product Pathways - Tyrosine Kinase / Adaptors

Human EGF Neutralizing (D8A1) Rabbit mAb #12157

Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
N 1:1 Human, Rabbit IgG

Species cross-reactivity is determined by western blot.

Applications Key: N=Neutralizing,

Specificity / Sensitivity

Human EGF Neutralizing (D8A1) Rabbit mAb binds to human EGF and neutralizes its effects in an MCF 10A cell proliferation assay. This antibody does not cross-react with human betacellulin or human TGF-α.

人EGF Neutralizing (D8A1) Rabbit mAb(中和兔单抗)能够与人源EGF相结合,并且能够在10A细胞增殖测试实验发挥中和EGF活性的作用。这种抗体不会与人源细胞调节素或者人源TGF-α发生交叉反应。

Source / Purification

Monoclonal antibody is produced by immunizing animals with a recombinant human EGF protein.

单克隆抗体通过重组人源EGF蛋白免疫动物制备。

Description

Neutralizing antibodies can be used to inhibit normal biological function through their binding to biological molecules. These reagents can be used to determine the effects that a particular molecule has in biological systems. Human EGF Neutralizing (D8A1) Rabbit mAb has been shown to neutralize the EGF-induced proliferation of MCF 10A cells in vitro with an ND50 in the range of 200-700 ng/ml.

中和抗体能够通过结合生物分子抑制正常的生物功能。这些试剂可以用来确定一个特定的大分子在生物系统中的影响。人EGF Neutralizing (D8A1) Rabbit mAb(中和兔单抗)能够中和体外EGF诱导的MCF 10A细胞的增殖,ND50的范围在200-700 ng/ml。

Bioactivity

Bioactivity

The proliferation of MCF 10A cells treated with increasing concentrations of hEGF #8916 was assessed. After 24 hr, cells were labeled with BrdU for 4 hr and BrdU incorporation was determined using BrdU Cell Proliferation Assay Kit #6813.MCF 10A细胞的增殖通过递增浓度的hEGF #8916处理进行评估。24小时后,用BrdU标记细胞4小时,BrdU的结合用 BrdU Cell Proliferation Assay Kit #6813测定。

Bioactivity

Bioactivity

The ability of Human EGF Neutralizing (D8A1) Rabbit mAb to inhibit hEGF-induced MCF 10A cell proliferation was assessed. MCF 10A cells were treated with increasing concentrations of antibody in the presence of hEGF #8916 (2 ng/ml). After 24 hr, cells were labeled with BrdU for 4 hr and BrdU incorporation was determined using BrdU Cell Proliferation Assay Kit #6813.Human EGF Neutralizing (D8A1) Rabbit mAb能够抑制hEGF诱导的MCF 10A细胞的增殖。MCF 10A细胞通过hEGF #8916 (2 ng/ml)浓度抗体处理。24小时后,细胞被用BrdU标记4小时,BrdU的结合用 BrdU Cell Proliferation Assay Kit #6813测定。

Background

EGF is produced by epithelial cells, fibroblasts, and many other cell types (1,2). Low molecular weight soluble EGF is generated through proteolysis of a larger ~130,000 kDa transmembrane precursor (1,2). Both soluble and membrane forms of EGF are active (2). EGF induces proliferation, differentiation, and survival of many cell types including tumor-derived cells (1-3). There are multiple members of the EGF family and multiple members of the HER/ErbB EGF receptor family. EGF binds to HER1/ErbB1 and induces homo- or heterodimerization with other HER/ErbB family members, resulting in signaling through the MAPK, PI3K/Akt, and Stat5 pathways (1). Research studies have implicated EGF, EGF family members, EGF receptors, and their signaling pathways in many cancers (1,2).

EGF是由上皮细胞、成纤维细胞以及很多其它细胞型产生的(1,2)。低分子量可溶性的EGF通过一个横跨膜前导蛋白的蛋白质水解产生(1,2)。可溶性的和细胞膜形式的EGF都是有活性的(2)。EGF诱导许多细胞类型,包括肿瘤衍生细胞的增殖、分化和存活(1-3)。EGF家族和HER/ErbB EGF受体家族都有很多成员。EGF能够与HER1/ErbB1结合并且诱导与HER1/ErbB1家族的同源二聚体和异源二聚体的形成,导致MAPK、PI3K/Akt和Stat5 信号通路的激活(1)。在很多癌症中,EGF、EGF家族成员、EGF受体以及它们的通路都相关(1,2)。

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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