Cell Signaling Technology

Product Pathways - MAPK Signaling

MAPKAPK-2 (D1E11) Rabbit mAb #12155

No. Size Price
12155S 100 µl ( 10 western blots ) ¥3,100.00 现货查询 购买询价
12155 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat,Monkey,Bovine,Pig, Endogenous 47, 49 (Phosphorylated) Rabbit IgG
IP 1:100

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation,

Homology

Species predicted to react based on 100% sequence homology: Hamster, Dog, Horse,

Specificity / Sensitivity

MAPKAPK-2 (D1E11) Rabbit mAb recognizes endogenous levels of total MAPKAPK-2 protein. This antibody is not known to cross-react with other MAPKAPK proteins.

MAPKAPK-2 (D1E11)Rabbit mAb兔单抗可以识别内源性总MAPKAPK-2蛋白。该抗体与其他MAPKAPK蛋白没有交叉反应。

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro381 of human MAPKAPK-2 protein.

该单克隆抗体是采用合成的与人源MAPKAPK-2蛋白Pro381附近氨基酸相一致的肽段免疫动物产生的。

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines, untreated (-) or treated with UV (40 mJ/cm2, 30 min recovery; +), using MAPKAPK-2 (D1E11) Rabbit mAb (upper) or GAPDH (D16H11) XP® Rabbit mAb #5174 (lower).未处理(-)或使用UV (40 mJ/cm2, 30 min recovery; +)处理的多种细胞提取物,使用MAPKAPK-2 (D1E11)Rabbit mAb(上)或GAPDH (D16H11) XP®Rabbit mAb#5174(下)。

IP

IP

Immunoprecipitation of MAPKAPK-2 from HeLa cell extracts using MAPKAPK-2 (D1E11) Rabbit mAb (lane 2), MAPKAPK-2 Antibody #3042 (lane 3), or Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 4). Lane 1 is 10% input. Western blot analysis was performed using MAPKAPK-2 (D1E11) Rabbit mAb. Mouse Anti-rabbit IgG (Conformation Specific) (L27A9) mAb #3678 was used as a secondary antibody.使用MAPKAPK-2 (D1E11)Rabbit mAb(lane2),MAPKAPK-2抗体#3042 (lane 3),或Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 4)对HeLa细胞提取物的MAPKAPK-2进行免疫沉淀实验。Lane 1是10%上样量。MAPKAPK-2 (D1E11)Rabbit mAb用作western blot分析。Mouse Anti-rabbit IgG (Conformation Specific) (L27A9) mAb #3678作为二抗。

Western Blotting

Western Blotting

Western blot analysis of cell extracts from MAPKAPK-2 wild type (WT) and knockout (KO) mouse embryonic fibroblasts (MEF) using MAPKAPK-2 (D1E11) Rabbit mAb (upper) or GAPDH (D16H11) XP® Rabbit mAb #5174 (lower). MAPKAPK-2 WT and KO MEFs were generously provided by Drs. Sandra Morandell and Michael Yaffe, David H. Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology, Cambridge, MA.MAPKAPK-2野生型(WT)和敲除(KO)小鼠胚胎成纤维细胞(MEF)的提取物使用MAPKAPK-2 (D1E11)Rabbit mAb(上)或GAPDH (D16H11) XP®兔mAb #5174(下)进行western blot分析。MAPKAPK-2 WT和KO MEFs由David H. Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology, Cambridge, MA的Drs. Sandra Morandell 和Michael Yaffe提供。

Background

In response to cytokines, stress, and chemotactic factors, MAP kinase-activated protein kinase 2 (MAPKAPK-2) is rapidly phosphorylated and activated. It has been shown that MAPKAPK-2 is a direct target of p38 MAPK (1). Multiple residues of MAPKAPK-2 are phosphorylated in vivo in response to stress. However, only four residues (Thr25, Thr222, Ser272, and Thr334) are phosphorylated by p38 MAPK in an in vitro kinase assay (2). Phosphorylation at Thr222, Ser272, and Thr334 appears to be essential for the activity of MAPKAPK-2 (2). Thr25 is phosphorylated by p42 MAPK in vitro, but is not required for the activation of MAPKAPK-2 (2).

在细胞因子、压力和化学因子的刺激下,丝裂原活化蛋白激酶激活的蛋白激酶2(MAPKAPK-2)能够迅速被磷酸化并被激活。研究显示MAPKAPK-2是p38 MAPK的直接靶点(1)。在体内,在压力应激下MAPKAPK-2的多个氨基酸都能被磷酸化。但是,在体外激酶实验中,仅有4个位点(Thr25, Thr222, Ser272, 和 Thr334)能够被p38 MAPK磷酸化(2)。Thr222, Ser272和Thr334的磷酸化对于MAPKAPK-2的激酶活性是必须的(2)。Thr25可以在体外被p42 MAPK磷酸化,但是该磷酸化对于MAPKAPK-2的激活不是必须的(2)。

  1. Rouse, J. et al. (1994) Cell 78, 1027-1037.
  2. Ben-Levy, R. et al. (1995) EMBO J. 14, 5920-5930.

Application References

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!

Protocols

Companion Products


For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

用户评论 --- 共 0

该产品暂无评论!

我要参与评论 :

如要参与评论请先登录网站

还没有网站账户?去注册一下吧

Products

 

Applications