Cell Signaling Technology

Product Pathways - Cytoskeletal Signaling

Acetyl-α-Tubulin (Lys40) (6-11B-1) Mouse mAb #12152

No. Size Price
12152S 100 µl ( 10 western blots ) ¥3,900.00 现货查询 购买询价
12152 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Rat, Endogenous 52 Mouse IgG2b
IP 1:50

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation,

Homology

Species predicted to react based on 100% sequence homology: Mouse,

Specificity / Sensitivity

Acetyl-α-Tubulin (Lys40) (6-11B-1) Mouse mAb recognizes endogenous levels of α-tubulin protein only when acetylated at Lys40.

Acetyl-α-Tubulin (Lys40) (6-11B-1)Mouse mAb可以识别Lys40被乙酰化的内源性α-tubulin蛋白。

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic acetylpeptide corresponding to residues surrounding Lys40 of human α-tubulin protein.

单克隆抗体由合成肽段免疫动物产生,该肽段与人α-tubulin蛋白的Lys40及其邻近氨基酸残基序列一致。

Western Blotting

Western Blotting

Western blot analysis of extracts from HeLa cells, untreated (-) or treated with TSA #9950 (400 nM, 16 hr; +), using Acetyl-α-Tubulin (Lys40) (6-11B-1) Mouse mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower).未处理(-)或经过TSA #9950 (400 nM, 16 hr; +)处理的HeLa细胞提取物,使用Acetyl-α-Tubulin (Lys40) (6-11B-1)Mouse mAb(上)或β-Actin (D6A8) Rabbit mAb #8457进行western blot分析。

Background

The cytoskeleton consists of three types of cytosolic fibers: microtubules, microfilaments (actin filaments), and intermediate filaments. Globular tubulin subunits comprise the microtubule building block, with α/β-tubulin heterodimers forming the tubulin subunit common to all eukaryotic cells. γ-tubulin is required to nucleate polymerization of tubulin subunits to form microtubule polymers. Many cell movements are mediated by microtubule action, including the beating of cilia and flagella, cytoplasmic transport of membrane vesicles, chromosome alignment during meiosis/mitosis, and nerve-cell axon migration. These movements result from competitive microtubule polymerization and depolymerization or through the actions of microtubule motor proteins (1).

细胞骨架包括三种细胞质纤维:微管,微丝(肌动蛋白丝)和中间丝。球形微管亚基由microtubule building block组成,在所有的真核细胞中,α/β-tubulin异二聚体形成的微管亚基是很常见的。γ-tubulin对于tubulin亚基的细胞核多聚体话以形成微管复合物是必须的。许多细胞运动都是通过微管的活动介导的,包括纤毛和鞭毛的跳动,胞质膜小泡的运输、减数分裂/有丝分裂时的染色质对齐,和神经细胞轴突迁移。这些运动是微管聚合和结局的竞争结果,或者是通过微管马达蛋白的作用而发生的(1)。

The Elongator complex catalytic subunit (Elp3) acetylates α-tubulin at Lys40, while the histone deacetylase HDAC6 functions as a tubulin deacetylase. This post-translational modification may be required for dynamic cell shape remodeling, cell motility, tubulin stability, and terminal branching of cortical neurons (2,3).

延伸复合物催化亚基(Elp3)乙酰化α-tubulin的Lys40,组蛋白去乙酰化酶HDAC6能够对tubulin去乙酰化。这种翻译后修饰可能是动态的细胞形态重塑,细胞运动,微管稳定,和皮质神经元的终端分支,所必须的(2,3)。

  1. Westermann, S. and Weber, K. (2003) Nat Rev Mol Cell Biol 4, 938-47.
  2. Creppe, C. et al. (2009) Cell 136, 551-64.
  3. Hubbert, C. et al. (2002) Nature 417, 455-8.

Application References

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!

Protocols

Companion Products


For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

用户评论 --- 共 0

该产品暂无评论!

我要参与评论 :

如要参与评论请先登录网站

还没有网站账户?去注册一下吧

Products

 

Applications