Cell Signaling Technology

Product Pathways - MAPK Signaling

PD184352 #12147

CI-1040   MAPK   MEK inhibitor   MEK1   MEK5  

Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype

Species cross-reactivity is determined by western blot.

Applications Key:

Molecular Characterization




Chemical structure of PD184352.PD184352的化学结构。

Western Blotting

Western Blotting

Western blot analysis of extracts from HeLa cells, serum-starved overnight and untreated or treated with TPA #4174 (200 nM, 20 min) either with or without PD184352 pretreatment (1 hr) at the indicated concentrations, using Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (D13.14.4E) XP® Rabbit mAb #4370 (upper) or p44/42 MAPK (Erk1/2) (137F5) Rabbit mAb #4695 (lower).Western blot分析HeLa细胞提取物,血清饥饿过夜,未处理或者先用PD184352 (1小时)根据设计好的不同浓度进行预处理或不预处理,再用TPA #4174 (200 nM, 20分钟)处理,使用的抗体是:Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (D13.14.4E) XP® Rabbit mAb #4370(上图)、p44/42 MAPK (Erk1/2) (137F5) Rabbit mAb #4695(下图)。

Directions for Use

PD184352 is supplied as a lyophilized powder. For a 10 mM stock, reconstitute the 5 mg in 1044.6 µl DMSO. Working concentrations and length of treatment can vary depending on the desired effect, but it is typically used as a pretreatment at 0.1-10 µM for 0.5-1 hr prior to treating with a stimulator or is used alone with varying treatment times lasting up to 24 hr. Soluble in DMSO at 25 mg/ml and ethanol at 8 mg/ml with warming; poorly soluble in water with maximum solubility in water at ~5-10 µM.

提供的PD184352为冻干粉。对于10mM浓度贮存液,在5 mg冻干粉中加入1044.6 µl DMSO。工作浓度和处理时间根据所需的预期效果来确定,一般在用兴奋剂处理前,以0.1-10 µM,处理0.5-1 小时或者单独使用,能作用不同的时间,可持续作用24小时。能溶于DMSO(25 mg/ml)和加热的乙醇(8 mg/ml),难溶于水,在水中的最大溶解度约为5-10µM。


PD184352 (CI-1040) is a highly potent and selective noncompetitive MEK inhibitor with an IC50 of 17 nM for MEK1 (1). Treatment of cells with PD184352 leads to suppression of MAPK activation (1-4) and potently inhibits the proliferation of cells harboring the B-Raf mutation V600E (2,3). Researchers have shown that PD184352 effectively inhibits MEK5, although not as potently as MEK1 (4).

PD184352 (CI-1040)是一种非常有效的选择性的非竞争性MEK抑制剂,MEK1的IC50为17nm(1)。用PD184352进行细胞的治疗会抑制MAPK的活化(1-4)并能有效抑制细胞的增殖,包含B-Raf基因V600E突变(2,3)。研究者发现PD184352能有效抑制MEK5,但是效果不如对MEK1的好(4)。

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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