Cell Signaling Technology

Product Pathways - Neuroscience

Brn2/POU3F2 (D2C1L) Rabbit mAb #12137

No. Size Price
12137S 100 µl ( 10 western blots ) ¥3,100.00 现货查询 购买询价
12137 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat, Endogenous 55 Rabbit IgG
IP 1:100
IF-F 1:3200
ChIP 1:50

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation, IF-F=Immunofluorescence (Frozen), ChIP=Chromatin IP,

Specificity / Sensitivity

Brn2/POU3F2 (D2C1L) Rabbit mAb recognizes endogenous levels of total Brn2/POU3F2 protein.

Brn2/POU3F2 (D2C1L) Rabbit mAb兔单抗能够检测内源性的Brn2/POU3F2总蛋白。

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human Brn2/POU3F2 protein.

该单克隆抗体是由合成的人源Brn2/POU3F2蛋白N末端肽段免疫动物而制备的。

Chromatin IP

Chromatin IP

Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 106 A-375 cells and either 10 μl of Brn2/POU3F2 (D2C1L) Rabbit mAb or 2 μl of Normal Rabbit IgG #2729 using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using SimpleChIP® Human MITF Promoter Primers #12377 and SimpleChIP® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.

染色质免疫共沉淀分析来源于4 x 106 A-375细胞的交联染色质。使用的抗体是10 μl的Brn2/POU3F2 (D2C1L) Rabbit mAb 兔单抗或 2 μl的Normal Rabbit IgG #2729,使用SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003。QPCR分析富集的DNA,使用试剂盒为SimpleChIP® Human MITF Promoter Primers #12377和SimpleChIP® Human α Satellite Repeat Primers #4486。在每个样品中免疫沉淀的DNA量被定义为与基因组内参对照(相当于1)总量相关的信号。

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using Brn2/POU3F2 (D2C1L) Rabbit mAb.

Western blot 检测多种细胞系提取物,使用的抗体是Brn2/POU3F2 (D2C1L) Rabbit mAb兔多抗。

IF-F

IF-F

Confocal immunofluorescent analysis of P1 rat brain using Brn2 (D7H3) Rabbit mAb (green). Actin filaments were labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). Gated area shown at 10X magnification on right.

共聚焦免疫荧光分析P1大鼠大脑,使用的抗体是Brn2 (D7H3) Rabbit mAb兔单抗 (绿色)。肌动蛋白纤丝用 DY-554 phalloidin (红色)标记. 蓝色伪彩= DRAQ5® #4084 (DNA荧光染料)。右图为10X 放大图。

Background

Brn2/POU3F2 is a POU domain-containing transcription factor involved in neuronal differentiation and activation of the corticotrophin-releasing hormone gene (1,2). In mice, disruption of the Brn2 gene results in loss of specific neuronal lineages in the hypothalamus (3). In addition to its role in mammalian neurogenesis, Brn2 has also been implicated in melanoma tumorigenesis and has been shown in the literature to be overexpressed in human melanoma cells compared to normal melanocytes (4,5). Recent studies also identify Brn2 as a transcription factor playing an important role in keratinocyte differentiation (6). Recent reports demonstrate that overexpression of three transcription factors (Brn2, Ascl1, and Myt1L) can directly convert human fibroblasts into functional neurons under precisely defined conditions (7,8).

Brn2/POU3F2是一个含POU结构域的转录因子,参与了神经细胞的分化和促肾上腺皮质激素释放激素基因的活化(1,2)。在小鼠中,Brn2基因的缺失会导致下丘脑中特定神经细胞谱系的丢失(3)。除了在哺乳动物神经元再生中的作用外,Brn2也与黑色素瘤肿瘤的发生相关,并已有文献证明在人黑色素瘤细胞中在表达量比正常的黑色素细胞要显著提高(4,5)。最近的研究还确定了Brn2作为一个转录因子在角质形成细胞分化中发挥了重要作用(6)。最近的报告表明,精确限定的条件下三种转录因子超量表达(Brn2,ASCL1和Myt1L)可以直接将人类成纤维细胞转换为有功能的神经元(7,8)。

  1. Fujii, H. and Hamada, H. (1993) Neuron 11, 1197-206.
  2. Schonemann, M.D. et al. (1995) Genes Dev 9, 3122-35.
  3. Nakai, S. et al. (1995) Genes Dev 9, 3109-21.
  4. Cook, A.L. et al. (2003) J Invest Dermatol 121, 1150-9.
  5. Cook, A.L. and Sturm, R.A. (2008) Pigment Cell Melanoma Res 21, 611-26.
  6. Shi, G. et al. (2010) PLoS One 5, e13216.
  7. Pfisterer, U. et al. (2011) Proc Natl Acad Sci USA 108, 10343-8.
  8. Ambasudhan, R. et al. (2011) Cell Stem Cell 9, 113-8.

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For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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