Cell Signaling Technology

Product Pathways - Autophagy Signaling

WIPI1 Antibody #12124

No. Size Price
12124S 100 µl ( 10 western blots ) ¥3,100.00 现货查询 购买询价
12124 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human, Endogenous 48 Rabbit

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting,

Homology

Species predicted to react based on 100% sequence homology: Monkey,

Specificity / Sensitivity

WIPI1 Antibody recognizes endogenous levels of total WIPI1 protein.

WIPI1 Antibody能够检测内源性的WIPI1总蛋白。

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Lys369 of human WIPI1 protein. Antibodies are purified by protein A and peptide affinity chromatography.

该多克隆抗体通过合成肽免疫动物制备,此合成肽的序列与人源的WIPI1蛋白Lys369周围的残基相一致。抗体通过蛋白A和多肽亲和色谱法纯化。

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using WIPI1 Antibody.

Westerm blot 分析多种细胞系的细胞提取物,使用抗体是WIPI1 Antibody。

Western Blotting

Western Blotting

Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with a construct expressing human WIPI1 (hWIPI1; +), using WIPI1 Antibody.

Western blot 分析293T细胞提取物,对照转染(-)或转染表达人源WIPI1的质粒(hWIPI1; +),使用抗体为WIPI1 Antibody。

Background

Autophagy is a catabolic process for the autophagosomic-lysosomal degradation of bulk cytoplasmic contents (1,2). It is generally activated by conditions of nutrient deprivation but is also associated with a number of physiological processes including development, differentiation, neurodegeneration, infection, and cancer (3). The molecular machinery of autophagy was largely discovered in yeast and is directed by a number of autophagy-related (Atg) genes. Vacuolar trafficking and autophagy are controlled by the class III type phosphoinositide 3-kinase (PI3K) Vps34, which generates phosphoinositide-3-phosphate (PtdIns3P) (4,5). Atg18 and Atg21 are two related WD-repeat proteins that bind PtdIns3P via a conserved Phe-Arg-Arg-Gly motif (6,7). It has been shown that Atg18 binds to Atg2 and that this complex is directed to vacuolar membranes by its interaction with PtdIns3P (8). Human orthologues of Atg18 and Atg21 were identified as members of the WD-repeat protein Interacting with PhosphoInositides (WIPI) family (9-11). WIPI1 (also called WIPI49) and WIPI2 have been shown to translocate from several vacuolar compartments to LC3-positive autophagosomes during autophagy; this translocation may be used as an autophagy marker (12).

自噬是自噬溶酶体对其包裹的细胞质内含物进行降解的一种分解代谢过程(1,2)。自噬过程一般是在营养匮乏的条件下被激活,但是也与一些生理过程有关,如发育、分化、神经变性、感染和癌症(3)。关于自噬的分子机制,已在酵母中被大量发现,且和一些自噬相关基因(Atg)有关。空泡运输和自噬是通过III型磷酸肌醇3-激酶(PI3K)Vsp34控制的,从而生成磷酸肌醇-3-磷酸(PtdIns3P) (4,5)。Atg18和Atg21是两种相关联的WD重复蛋白,它们可以通过保守的Phe-Arg-Arg-Gly序列结合PtdIns3P (6,7)。研究表明,Atg18可以与Atg2结合,并且这种复合物通过与PtdIns3P的相互作用被转到空泡膜处(8)。人的Atg18和Atg21的同源基因被认为是与磷酸肌醇家族相互作用的WD-重复蛋白的成员(9-11)。研究证明,在自噬过程中,WIPI1(又称WIPI49)和WIPI2可以从几个空泡间隔中转移到LC3阳性自噬体中,这种转移或许可以作为自噬的标志(12)。

  1. Reggiori, F. and Klionsky, D.J. (2002) Eukaryot Cell 1, 11-21.
  2. Codogno, P. and Meijer, A.J. (2005) Cell Death Differ 12 Suppl 2, 1509-18.
  3. Levine, B. and Yuan, J. (2005) J Clin Invest 115, 2679-88.
  4. Corvera, S. (2001) Traffic 2, 859-66.
  5. Yan, Y. and Backer, J.M. (2007) Biochem Soc Trans 35, 239-41.
  6. Krick, R. et al. (2006) FEBS Lett 580, 4632-8.
  7. Strømhaug, P.E. et al. (2004) Mol Biol Cell 15, 3553-66.
  8. Obara, K. et al. (2008) J Biol Chem 283, 23972-80.
  9. Jeffries, T.R. et al. (2004) Mol Biol Cell 15, 2652-63.
  10. Proikas-Cezanne, T. et al. (2007) FEBS Lett 581, 3396-404.
  11. Polson, H.E. et al. (2010) Autophagy 6, 506-22.

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Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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