Cell Signaling Technology

Product Pathways - Lymphocyte Signaling

Bcl-11B (D6F1) XP® Rabbit mAb #12120

No. Size Price
12120S 100 µl ( 10 western blots ) ¥3,580.00 现货查询 购买询价
12120T 20 µl ( 2 western blots ) ¥1,400.00 现货查询 购买询价
12120 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human, Endogenous 120-130 Rabbit IgG
IP 1:100
IF-IC 1:200
ChIP 1:50

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation, IF-IC=Immunofluorescence (Immunocytochemistry), ChIP=Chromatin IP,

Homology

Species predicted to react based on 100% sequence homology: Mouse, Rat, Monkey,

Specificity / Sensitivity

Bcl-11B (D6F1) XP® Rabbit mAb recognizes endogenous levels of total Bcl-11B protein.

Bcl-11B (D6F1) XP®Rabbit mAb可以识别内源性的总Bcl-11B蛋白。

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu391 of human Bcl-11B protein.

合成对应人Bcl-11B蛋白邻近Leu391氨基酸残基的多肽免疫动物获得多克隆抗体。

IF-IC

IF-IC

Confocal immunofluorescent analysis of Jurkat (positive; left) and HeLa (negative; right) cells using Bcl-11B (D6F1) XP® Rabbit mAb (green). Actin filaments were labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

使用Bcl-11B (D6F1) XP®Rabbit mAb(绿色)对Jurkat(阳性;左)和HeLa(阴性;右)细胞进行激光共聚焦免疫荧光分析。肌动蛋白丝使用DY-554 phalloidin 标记(红色)。蓝色伪彩= DRAQ5® #4084 (DNA荧光染色)。

Chromatin IP

Chromatin IP

Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 106 Jurkat cells and either 10 μl of Bcl-11B (D6F1) XP® Rabbit mAb or 2 μl of Normal Rabbit IgG #2729 using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using SimpleChIP® Human CDKN1B Promoter Primers #11951, human MDM2 promoter primers, and SimpleChIP® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.

使用SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003中的10 μl Bcl-11B (D6F1) XP® Rabbit mAb兔单抗或2 μl Normal Rabbit IgG #2729与4 x 106 Jurkat交联后进行进行染色质免疫共沉淀实验。富集的DNA使用SimpleChIP® Human CDKN1B Promoter 引物 #11951, human MDM2 引物, 和SimpleChIP® Human α Satellite Repeat 引物 #4486进行实时荧光定量PCR实验。各样品沉淀得到的DNA量比对input染色质总量进行定量,Input染色质量设定值为1。

Western Blotting

Western Blotting

Western blot analysis of extracts from MOLT-4 and Jurkat cells using Bcl-11B (D6F1) XP® Rabbit mAb.

使用Bcl-11B (D6F1) XP® Rabbit mAb对MOLT-4和Jurkat细胞提取物进行western blot分析。

IP

IP

Immunoprecipitation of Bcl-11B from MOLT-4 cell extracts, using Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) or Bcl-11B (D6F1) XP® Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using Bcl-11B (D6F1) XP® Rabbit mAb.

使用(DA1E) mAb IgG XP® Isotype Control #3900 (lane 2)或Bcl-11B (D6F1) XP®Rabbit mAb (lane 3)对MOLT-4细胞提取物中的Bcl-11B蛋白进行免疫沉淀实验。Lane1是10%上样量。使用Bcl-11B (D6F1) XP®Rabbit mAb兔单抗进行western blot分析。

Background

Bcl-11B (Ctip2) is a COUP-TF interacting protein that belongs to the C2H2 type zinc finger protein family (1). Bcl-11B is highly expressed in the brain and is critical for the development of neurons, as well as other tissues and organs. Bcl-11B also plays an essential role in T cell lineage commitment and maintenance of T cell identity (1-3). Two isoforms of Bcl-11B are found to be encoded by the BCL11B gene, possibly through exon-skipping (4). Bcl-11B is a transcription factor which binds to target genes through the 2nd and 3rd zinc-finger domains of exon 4 (3), while also interacting with various protein partners including COUP-TF proteins (1), the NuRD complex (5,6), HDAC1, HDAC2, and SUV39H1 (7). Research studies have shown that mutations and deletion of Bcl-11B contribute to the development of thymic lymphoma in mice and T cell acute lymphoblastic leukemia in humans, indicating a role as a tumor suppressor (4,8). Mechanistic studies have shown that Bcl-11B represses gene expression of the E3 ubiquitin ligase HDM2 in a p53-dependent manner (9).

Bcl-11B (Ctip2)是一个COUP-TF作用蛋白,属于C2H2型锌指蛋白家族(1)。Bcl-11B在大脑中高表达,对于神经和其他组织和器官的发育是必须的。 Bcl-11B在T细胞系的功能和维护T细胞身份也扮演重要角色(1-3)。BCL11B 基因可能通过外显子的不同转录编码了BCL-11B的两种异构型蛋白。BCL-11B是一类通过其第4外显子(3)中第二和第三锌指结构域结合靶向基因的转录因子,同时也与包括COUP-TF proteins (1), the NuRD complex (5,6), HDAC1, HDAC2和 SUV39H1 (7)等多种蛋白伴侣相互作用。 已有研究发现Bcl-11B突变和丢失会导致小鼠的胸腺肿瘤的发生和人类的T细胞急性粒细胞白血病,显示其抑癌基因的角色(4,8)。研究机制发现Bcl-11B非依赖于P53 的抑制E3泛素连接酶HDM2的基因表达(9)。

  1. Avram, D. et al. (2000) J Biol Chem 275, 10315-22.
  2. Liu, P. et al. (2010) Immunol Rev 238, 138-49.
  3. Kominami, R. (2012) Proc Jpn Acad Ser B Phys Biol Sci 88, 72-87.
  4. Wakabayashi, Y. et al. (2003) Biochem Biophys Res Commun 301, 598-603.
  5. Cismasiu, V.B. et al. (2005) Oncogene 24, 6753-64.
  6. Topark-Ngarm, A. et al. (2006) J Biol Chem 281, 32272-83.
  7. Marban, C. et al. (2007) EMBO J 26, 412-23.
  8. Gutierrez, A. et al. (2011) Blood 118, 4169-73.
  9. Obata, M. et al. (2012) Cell Signal 24, 1047-52.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

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Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

XP is a registered trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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