Cell Signaling Technology

Product Pathways - Metabolism

PathScan® Phospho-Acetyl-CoA Carboxylase (Ser79) Chemiluminescent Sandwich ELISA Kit #12116

ACC ELISA   P-ACC ELISA  

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No. Size Price
12116C 1 Kit ( 96 assays ) ¥6,345.00 现货查询 购买询价
Product Includes Volume Solution Color
ELISA Sample Diluent 25 ml Blue
Anti-mouse IgG, HRP-linked Antibody (ELISA Formulated) 1 ea Red (Lyophilized)
Detection Antibody Diluent 5.5 ml Green
HRP Diluent 5.5 ml Red
Acetyl-CoA Carboxylase Mouse Detection mAb 1 ea Green (Lyophilized)
Luminol/Enhancer Solution 3 ml Colorless
PathScan® Sandwich ELISA Lysis Buffer (1X) #7018 30 ml Colorless
ELISA Wash Buffer (20X) 25 ml Colorless
Sealing Tape 2 sheets
Stable Peroxide Buffer 3 ml Colorless
Phospho-ACC (Ser79) Rabbit Ab Coated Microwells 96 tests

Specificity / Sensitivity

PathScan® Phospho-Acetyl-CoA Carboxylase (Ser79) Chemiluminescent Sandwich ELISA Kit recognizes endogenous levels of phospho-ACC (Ser79) in human cells. This kit detects proteins from the indicated species, as determined through in-house testing, but may also detect homologous proteins from other species.

PathScan® Phospho-Acetyl-CoA Carboxylase (Ser79) Chemiluminescent Sandwich ELISA Kit 能够检测人的细胞中内源性的磷酸化-ACC (Ser79)水平。本试剂盒可检测经内部测试确定的指定物种的蛋白质,同时也可以检测其他物种的同源蛋白。

Description

The PathScan® Phospho-Acetyl-CoA Carboxylase (Ser79) Chemiluminescent Sandwich ELISA Kit is a solid phase sandwich enzyme-linked immunosorbent assay (ELISA) that detects endogenous levels of acetyl-CoA carboxylase (ACC) protein phosphorylated at Ser79 with a chemiluminescent readout. Chemiluminescent ELISAs often have a wider dynamic range and higher sensitivity than conventional chromogenic detection. This chemiluminescent ELISA, which is offered in low volume microplates, shows increased signal and sensitivity while using smaller samples. A Phospho-ACC (Ser79) Rabbit Antibody has been coated onto the microwells. After incubation with cell lysates, phospho-ACC protein is captured by the coated antibody. Following extensive washing, an ACC Mouse Detection mAb is added to detect the captured ACC protein. Anti-mouse IgG, HRP-linked Antibody is then used to recognize the bound detection antibody. Chemiluminescent reagent is added for signal development. The magnitude of light emission, measured in relative light units (RLU), is proportional to the quantity of phospho-ACC (Ser79) protein. 
 Antibodies in kit are custom formulations specific to kit.

The PathScan® Phospho-Acetyl-CoA Carboxylase (Ser79) Chemiluminescent Sandwich ELISA Kit采用了固相夹心酶联免疫吸附方法(ELISA)通过化学发光读数检测内源性水平的乙酰辅酶A羧化酶(ACC)蛋白Ser79磷酸化情况。与传统的显色检测法相比,化学发光ELISAs往往有着更宽泛的动态范围和更高的灵敏度。这种化学发光ELISA采用低体积的微孔板,在加强信号和灵敏度的同时需要的样本量更少。微孔中包被了Phospho-ACC (Ser79) Rabbit Antibody。细胞裂解液孵育后,磷酸化ACC蛋白被包被的抗体捕获。充分洗涤后,添加ACC Mouse Detection mAb检测捕获的ACC蛋白。再用Anti-mouse IgG, HRP-linked Antibody识别结合的抗体。添加化学发光试剂增强信号。测得的相对光单位(RLU)与磷酸化ACC(Ser79)蛋白的量成正比。试剂盒中的抗体为该试剂盒所特有。

Figure 1. Relationship between protein concentration of lysates from untreated and H2O2-treated Hep G2 cells and immediate light generation with chemiluminescent substrate is shown. Hep G2 cells (80-90% confluent) were treated with H2O2 (10 mM, 10 min) and lysed with PathScan® Sandwich ELISA Lysis Buffer (1X) #7018. Graph inset corresponding to the shaded area shows high sensitivity and a linear response at the low protein concentration range.

图1。如图所示未处理和H2O2处理Hep G2细胞裂解液蛋白浓度与化学发光底物即发光间的关系 。过氧化氢(10 mM, 10 min)处理Hep G2细胞(80-90%铺满)后,用PathScan® Sandwich ELISA Lysis Buffer (1X) #7018裂解。 插图阴影区域展示了高灵敏度以及低蛋白质浓度范围时的线性响应。

Background

Acetyl-CoA carboxylase (ACC) catalyzes the carboxylation of acetyl-CoA to malonyl-CoA (1). It is the key enzyme in the biosynthesis and oxidation of fatty acids (1). In rodents, the 265 kDa ACC1 (ACCα) form is primarily expressed in lipogenic tissues, while 280 kDa ACC2 (ACCβ) is the main isoform in oxidative tissues (1,2). However, in humans, ACC2 is the predominant isoform in both lipogenic and oxidative tissues (1,2). Phosphorylation by AMPK at Ser79 or by PKA at Ser1200 inhibits the enzymatic activity of ACC (3). ACC is a potential target of anti-obesity drugs (4,5).

乙酰辅酶A羧化酶(ACC)催化乙酰辅酶A羧化形成丙二酰辅酶A(1)。这是脂肪酸生物合成和氧化过程中的关键酶(1)。在啮齿类动物中,脂肪组织中主要表达的是265 kDa的ACC1(ACCα),而280 kDa的ACC2(ACCβ)是氧化组织中的主要构型(1,2)。然而,在人体中,ACC2是在脂肪和氧化组织均是主要构型(1,2)。AMPK催化的Ser79磷酸化与PKA催化的 Ser1200磷酸化会抑制ACC的酶活性(3)。ACC是抗肥胖药物的潜在靶点(4,5)。

  1. Castle, J.C. et al. (2009) PLoS One 4, e4369.
  2. Kreuz, S. et al. (2009) Diabetes Metab Res Rev 25, 577-86.
  3. Ha, J. et al. (1994) J Biol Chem 269, 22162-8.
  4. Abu-Elheiga, L. et al. (2001) Science 291, 2613-6.
  5. Levert, K.L. et al. (2002) J Biol Chem 277, 16347-50.

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For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

PathScan is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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