Cell Signaling Technology

Product Pathways - Neuroscience

β-Amyloid (1-39 Specific) (D5Y9L) Rabbit mAb #12077

No. Size Price
12077S 100 µl ( 10 western blots ) ¥4,050.00 现货查询 购买询价 防伪查询
12077 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human, Recombinant 4 Rabbit IgG

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting,


Species predicted to react based on 100% sequence homology: Mouse, Rat, Monkey, Bovine,

Specificity / Sensitivity

β-Amyloid (1-39 Specific) (D5Y9L) Rabbit mAb recognizes the Aβ-39 isoform of the β-amyloid peptides.

β-Amyloid (1-39 Specific) (D5Y9L) Rabbit mAb兔单抗能够检测内源性的β-淀粉样肽的Aβ-39 亚型。

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues at the carboxy terminus of human β-amyloid (1-39) peptide.


Western Blotting

Western Blotting

Western blot analysis of the indicated amounts of human Aβ-39 peptide using β-Amyloid (1-39 Specific) (D5Y9L) Rabbit mAb.

Western blot 定量检测人 Aβ-39 肽段,使用的抗体为β-Amyloid (1-39 Specific) (D5Y9L) Rabbit mAb兔单抗。

Western Blotting

Western Blotting

Western blot analysis of human Aβ-37, Aβ-38, Aβ-39, Aβ-40, Aβ-42, and Aβ-43 peptides (10 ng) using β-Amyloid (1-39 Specific) (D5Y9L) Rabbit mAb (upper) or β-Amyloid (D54D2) XP® Rabbit mAb #8243 (lower).

Western blot 检测人Aβ-37,Aβ-38,Aβ-39,Aβ-40,Aβ-42和 Aβ-43 肽段(10 ng),使用的抗体为β-Amyloid (1-39 Specific) (D5Y9L) Rabbit mAb兔单抗(上图)或β-Amyloid (D54D2) XP® Rabbit mAb #8243兔单抗(下图)。


Amyloid β (Aβ) precursor protein (APP) is a 100-140 kDa transmembrane glycoprotein that exists as several isoforms (1). The amino acid sequence of APP contains the amyloid domain, which can be released by a two-step proteolytic cleavage (1). The extracellular deposition and accumulation of the released Aβ fragments form the main components of amyloid plaques in Alzheimer's disease (AD) (1). APP can be phosphorylated at several sites, which may affect the proteolytic processing and secretion of this protein (2-5). Phosphorylation at Thr668 (a position corresponding to the APP695 isoform) by cyclin-dependent kinase is cell-cycle dependent and peaks during G2/M phase (4). APP phosphorylated at Thr668 exists in adult rat brain and correlates with cultured neuronal differentiation (5,6).

β淀粉样蛋白(Amyloid β,Aβ)前体蛋白(Amyloid β precursor protein, APP)是分子量为100-140 kDa的跨膜糖蛋白,它拥有几个亚型(1)。APP的氨基酸序列中所包含的淀粉样蛋白域(A-β),能够通过两步蛋白裂解反应被释放出来(1)。释出的A-β片段在细胞外沉积积累,形成了阿尔茨海默病(Alzheimer's disease,AD)中淀粉样斑块的主要组成部分(1)。APP存在多个能够被磷酸化的位点,这些位点影响着蛋白本身的裂解和分泌过程(2-5)。细胞周期蛋白依赖性激酶介导的Thr688(对应于APP695亚型)磷酸化是细胞周期依赖的,在G2/M期达到峰值(4)。APP的Thr668磷酸化形式存在于成年大鼠大脑和相应的培养神经细胞及其分化细胞中(5,6)。

  1. Selkoe, D.J. (1996) J Biol Chem 271, 18295-8.
  2. Caporaso, G.L. et al. (1992) Proc Natl Acad Sci USA 89, 3055-9.
  3. Hung, A.Y. and Selkoe, D.J. (1994) EMBO J 13, 534-42.
  4. Suzuki, T. et al. (1994) EMBO J 13, 1114-22.
  5. Ando, K. et al. (1999) J Neurosci 19, 4421-7.
  6. Iijima, K. et al. (2000) J Neurochem 75, 1085-91.

Application References

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Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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