Cell Signaling Technology

Product Pathways - Ca / cAMP / Lipid Signaling

SPHK1 (D1H1L) Rabbit mAb #12071

sc-48825   sphingosine kinase  

No. Size Price
12071S 100 µl ( 10 western blots ) ¥3,100.00 现货查询 购买询价
12071 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human, Endogenous 45-60 Rabbit IgG
IP 1:50

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation,

Specificity / Sensitivity

SPHK1 (D1H1L) Rabbit mAb recognizes endogenous levels of total SPHK1 protein. This antibody also cross-reacts with a protein of unknown origin at 160 kDa in some cell lines.

SPHK1 (D1H1L) Rabbit mAb兔单抗识别内源性水平的总SPHK1蛋白。在一些细胞系中,该抗体也能与一些未知区域的160 kDa蛋白发生交叉反应。

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro374 of human SPHK1 protein.

该单克隆抗体通过用合成磷酸肽免疫动物制备,该合成肽与人SPHK1蛋白脯氨酸(374位)附近的残基一样。

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using SPHK1 (D1H1L) Rabbit mAb.

Western blot方法检测多个细胞系的提取物,使用的抗体为SPHK1 (D1H1L) Rabbit mAb.

IP

IP

Immunoprecipitation of SPHK1 from HeLa cell extracts using Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) or SPHK1 (D1H1L) Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using SPHK1 (D1H1L) Rabbit mAb.

免疫沉淀法检测来自HeLa细胞提取物的SPHK1,使用的抗体为Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (泳道 2) 或SPHK1 (D1H1L) Rabbit mAb (泳道3). 泳道1是加入10%蛋白。进行western blot方法检测,使用的抗体为SPHK1 (D1H1L) Rabbit mAb.

Background

Sphingosine kinases (SPHKs) catalyze the phosphorylation of sphingosine to form sphingosine-1-phosphate (S1P), a lipid mediator with both intra- and extracellular functions. Together with other sphingolipid metabolizing enzymes, SPHKs regulate the balance of the lipid mediators, ceramide, sphingosine, and S1P (1-4). Two distinct SPHK isoforms, SPHK1 and SPHK2, have been cloned and characterized (5,6). SPHK1 and SPHK2 are highly conserved and diversely expressed (7,8). The SPHKs are activated by G protein-coupled receptors, receptor tyrosine kinases, immunoglobulin receptors, cytokines, and other stimuli (9-12). The molecular mechanisms by which SPHK1 and SPHK2 are specifically regulated are complex and only partially understood.

鞘氨醇激酶(SPHKs)催化鞘氨醇磷酸化形成鞘氨醇-1 - 磷酸(S1P),作为细胞内和细胞外功能的脂质载体。与其他的神经鞘脂代谢酶一样,SPHKs调节脂质载体、神经酰胺、鞘氨醇和S1P的平衡(1-4)。已经克隆和阐明两个截然不同的SPHK亚型,SPHK1和SPHK2(5,6)。SPHK1 和 SPHK2高度保守并广泛表达(7,8)。SPHKs都是由G蛋白偶联受体、受体酪氨酸激酶、免疫球蛋白受体、细胞因子和其他刺激物激活(9-12)。通过SphK1和SPHK2特异性调节的分子机制是复杂的,仅有部分已有阐述。

  1. Hait, N.C. et al. (2006) Biochim Biophys Acta 1758, 2016-26.
  2. Xia, P. et al. (2000) Curr Biol 10, 1527-30.
  3. Hannun, Y.A. et al. (2001) Biochemistry 40, 4893-903.
  4. Futerman, A.H. and Riezman, H. (2005) Trends Cell Biol 15, 312-8.
  5. Kohama, T. et al. (1998) J Biol Chem 273, 23722-8.
  6. Liu, H. et al. (2000) J Biol Chem 275, 19513-20.
  7. Liu, H. et al. (2002) Prog Nucleic Acid Res Mol Biol 71, 493-511.
  8. Spiegel, S. and Milstien, S. (2003) Nat Rev Mol Cell Biol 4, 397-407.
  9. Alemany, R. et al. (2007) Naunyn Schmiedebergs Arch Pharmacol 374, 413-28.
  10. Saba, J.D. and Hla, T. (2004) Circ Res 94, 724-34.
  11. Anliker, B. and Chun, J. (2004) J Biol Chem 279, 20555-8.
  12. Wattenberg, B.W. et al. (2006) J Lipid Res 47, 1128-39.

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Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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