Cell Signaling Technology

Product Pathways - Adhesion

Paxillin (D9G12) Rabbit mAb #12065

sc-5574  

No. Size Price
12065S 100 µl ( 10 western blots ) ¥3,100.00 现货查询 购买询价
12065 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human, Endogenous 54, 62, 68 Rabbit IgG
IP 1:50

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation,

Specificity / Sensitivity

Paxillin (D9G12) Rabbit mAb recognizes endogenous levels of total paxillin protein. The antibody recognizes all isoforms of human paxillin.

Paxillin (D9G12) Rabbit mAb用于检测内源性的总桩蛋白。该抗体识别所有的人桩蛋白同源异构体。

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Lys193 of human paxillin protein.

该单克隆抗体由人桩蛋白193位赖氨酸附近的残基合成多肽免疫动物产生。

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using Paxillin (D9G12) Rabbit mAb. The 45 kDa band is a degradation product of full-length paxillin protein.Western blot分析多种细胞系提取物,采用的抗体是Paxillin (D9G12) Rabbit mAb。45 kDa条带为全长桩蛋白的降解产物。

Background

Paxillin is a multidomain protein that localizes primarily to focal adhesion sites in the extracellular matrix (1). Paxillin is one of the key components of integrin signaling, and tyrosine phosphorylation of paxillin is required for integrin-mediated cytoskeletal reorganization (2). Paxillin is phosphorylated by another focal adhesion component, focal adhesion kinase (FAK), at Tyr118 (3,4). Phospho-FAK (Tyr118) may provide a docking site for recruitment of other signaling molecules to focal adhesions. It has been shown that the SH2 domain of Crk binds to the phosphorylated Tyr118 of paxillin (5).

桩蛋白是最初定位于细胞外基质粘附位点的多结构域蛋白(1)。桩蛋白是整合素信号转导的重要成员,桩蛋白的酪氨酸磷酸化需要整合素介导的细胞骨架的识别(2)。桩蛋白可以被位于118位酪氨酸的其它黏着斑组分磷酸化,黏着斑激酶(FAK)(3,4)。磷酸化-FAK(Tyr118)可能为其它信号转导分子的黏着斑提供一个停泊位点。已经表明Crk 的SH2结构域与桩蛋白的Tyr118磷酸化结合(5)。

  1. Turner, C.E. (2000) J Cell Sci 113 Pt 23, 4139-40.
  2. Burridge, K. et al. (1992) J Cell Biol 119, 893-903.
  3. Bellis, S.L. et al. (1995) J Biol Chem 270, 17437-41.
  4. Bellis, S.L. et al. (1997) Biochem J 325 ( Pt 2), 375-81.
  5. Schaller, M.D. and Parsons, J.T. (1995) Mol Cell Biol 15, 2635-45.

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For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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