Cell Signaling Technology

Product Pathways - Chromatin Regulation / Epigenetics

Tip60 Antibody #12058


No. Size Price
12058S 100 µl ( 10 western blots ) ¥3,250.00 现货查询 购买询价 防伪查询
12058 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human, Transfected Only 60 Rabbit

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting,


Species predicted to react based on 100% sequence homology: Mouse, Monkey, Bovine, Guinea Pig, Horse,

Specificity / Sensitivity

Tip60 Antibody recognizes transfected levels of total Tip60 protein.

Tip60 Antibody能够检测转染的Tip60总蛋白水平。

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding His60 of human Tip60 protein. Antibodies are purified by protein A and peptide affinity chromatography.

该多克隆抗体经采用合成的与人源Tip60蛋白 His60残基周围序列相对应的肽段免疫动物而生产的。由蛋白质A和多肽亲和层析纯化而成。

Western Blotting

Western Blotting

Western blot analysis of extracts from HeLa cells, mock transfected (-) or transfected with a construct expressing full-length human Tip60 (hTip60; +), using Tip60 Antibody.Western blot方法检测HeLa细胞提取物,分别用模拟物(-)或构建的全长人Tip60 (hTip60; +)转染,使用的抗体为 Tip60 Antibody。


Tip60 is a member of the MYST (MOZ, YBF2, SAS2 and Tip60) family of histone acetyltransferases and plays a role in a variety of cellular processes such as transcriptional regulation, DNA repair, and apoptosis (1,2). Tip60 exists as part of a multi-subunit complex that includes proteins such as TRRAP, p400, Reptin, and Pontin (3,4). Tip60 plays important roles in double-stranded DNA break (DSB) repair. Tip60 is required for the activation of the ATM kinase in response to DSBs, as well as acetylation of histones H4 and H2A.X at DSBs to facilitate DNA repair (1,2,5-7). In addition, Tip60 dependent acetylation at Lys120 of p53 within the DNA binding domain is required for the induction of apoptosis upon DNA damage (8,9). Tip60 is involved in a number of transcriptional regulation pathways driven by factors such as nuclear receptors and β-catenin (10-13). The Tip60 complex has been shown to be important for mouse embryonic stem cell self-renewal by regulating transcription of developmental regulators that are controlled by Nanog (14). GSK3 (glycogen synthase kinase-3) mediated phosphorylation at Ser86 of Tip60 promotes Tip60 acetylation and subsequent stimulation of the required autophagy protein ULK1 (15).

Tip60是组蛋白乙酰转移酶 MYST (MOZ, YBF2, SAS2和Tip60)家族的成员,在许多细胞进程如转录调控、DNA修复以及细胞凋亡中发挥作用(1,2)。Tip60作为包括TRRAP, p400, Reptin,和 Pontin蛋白多亚基复合物的一部分(3,4)。Tip60是double-stranded DNA break (DSB)修复中的一个角色。Tip60是响应DSB的ATM激酶激活所需要的,同时也是组蛋白H4 和H2A.X上 DSB乙酰化以帮助DNA修复所需要的(1,2,5-7)。此外p53 DNA结合结构域上Tip60依赖的Lys120位点乙酰化对于诱导DNA损伤后的细胞凋亡所需要的(8,9)。Tip60还参与许多由核受体和β-连环蛋白驱动的转录调控信号通路(10-13)。研究发现,Tip60复合物可以调控Nanog控制的发育调控子的转录,因此在小鼠胚胎干细胞自我更新中具有重要作用(14)。GSK3 (glycogen synthase kinase-3)介导的Tip60 Ser86位点磷酸化能够促进Tip60的乙酰化和随后对自噬所需蛋白ULK1的刺激(15)。

  1. Sun, Y. et al. (2010) Cell Cycle 9, 930-6.
  2. Fischle, W. (2009) Nat Cell Biol 11, 1279-81.
  3. Cai, Y. et al. (2003) J Biol Chem 278, 42733-6.
  4. Cai, Y. et al. (2005) J Biol Chem 280, 13665-70.
  5. Sun, Y. et al. (2005) Proc Natl Acad Sci U S A 102, 13182-7.
  6. Murr, R. et al. (2006) Nat Cell Biol 8, 91-9.
  7. Ikura, T. et al. (2007) Mol Cell Biol 27, 7028-40.
  8. Tang, Y. et al. (2006) Mol Cell 24, 827-39.
  9. Sykes, S.M. et al. (2006) Mol Cell 24, 841-51.
  10. Gaughan, L. et al. (2002) J Biol Chem 277, 25904-13.
  11. Jeong, K.W. et al. (2011) Nat Struct Mol Biol 18, 1358-65.
  12. Brady, M.E. et al. (1999) J Biol Chem 274, 17599-604.
  13. Kim, J.H. et al. (2005) Nature 434, 921-6.
  14. Fazzio, T.G. et al. (2008) Cell 134, 162-74.
  15. Lin, S.Y. et al. (2012) Science 336, 477-81.

Application References

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