Cell Signaling Technology

Product Pathways - TGF-beta/Smad Signaling

Smad2/3 Control Cell Extracts #12052

extract   lysate   smad   smad2   smad23   smad3  

No. Size Price
12052S 100 µl ( 10 western blots ) ¥1,415.00 现货查询 购买询价
12052 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype

Species cross-reactivity is determined by western blot.

Applications Key:

Description

Nonphosphorylated Smad2/3 Control Cell Extracts: Total cell extracts from HT-1080 cells, serum-starved overnight without treatment, serve as a negative control. Supplied in SDS Sample Buffer. Phosphorylated Smad2/3 Control Cell Extracts: Total cell extracts from HT-1080 cells, serum-starved overnight and treated with hTGF-β3 #8425 (10 ng/ml, 30 min), serve as a positive control. Supplied in SDS Sample Buffer.

非磷酸化Smad2/3 对照细胞提取物:HT-1080细胞全细胞提取物,血清饥饿过夜未处理作为阴性对照。以SDS样品缓冲液的形式提供。磷酸化Smad2/3 对照细胞提取物:HT-1080细胞全细胞提取物,血清饥饿过夜并经过hTGF-β3 #8425 (10 ng/ml, 30 min)处理作为阳性对照。以SDS样品缓冲液的方式提供。

Western Blotting

Western Blotting

Western blot analysis of Smad2/3 Control Cell Extracts from HT-1080 cells, untreated (-) or treated with hTGF-β3 #8425 (10 ng/ml, 30 min; +), using Phospho-Smad2 (Ser465/467) (138D4) Rabbit mAb #3108 (upper) or Smad2 (86F7) Rabbit mAb #3122 (lower).

未处理(-)或经过hTGF-β3 #8425 (10 ng/ml, 30 min; +)处理的HT-1080细胞,获取的细胞提取物作为Smad2/3对照,使用Phospho-Smad2 (Ser465/467) (138D4)Rabbit mAb 兔单抗#3108(上)或Smad2(86F7)Rabbit mAb兔单抗#3122进行western blot分析。

Western Blotting

Western Blotting

Western blot analysis of Smad2/3 Control Cell Extracts from HT-1080 cells, untreated (-) or treated with hTGF-β3 #8425 (10 ng/ml, 30 min; +), using Phospho-Smad3 (Ser423/425) (C25A9) Rabbit mAb #9520 (upper) or Smad3 (C67H9) Rabbit mAb #9523 (lower).

未处理(-)或经过hTGF-β3 #8425 (10 ng/ml, 30 min; +)处理HT-1080细胞,获取细胞提取物作为Smad2/3对照,使用Phospho-Smad3 (Ser423/425) (C25A9)Rabbit Rabbit mAb兔单抗 #9520(上)或Smad3 (C67H9)Rabbit mAb兔单抗 #9523(下)进行western blot分析。

Applications

As controls, we recommend using 10 μl of nonphosphorylated and phosphorylated Smad2/3 Control Cell Extracts. Boil for 2 minutes in the original tube, then load 10 μl per lane.

作为对照,我们推荐使用10μl 未磷酸化和磷酸化的Smad2/3对照细胞提取物。原管煮沸2分钟后,每个泳道中加入10μl样品。

Background

Members of the Smad family of signal transduction molecules are components of a critical intracellular pathway that transmit TGF-β signals from the cell surface into the nucleus. Three distinct classes of Smads have been defined: the receptor-regulated Smads (R-Smads), which include Smad1, 2, 3, 5, and 8; the common-mediator Smad (co-Smad), Smad4; and the antagonistic or inhibitory Smads (I-Smads), Smad6 and 7 (1-5). Activated type I receptors associate with specific R-Smads and phosphorylate them on a conserved carboxy terminal SSXS motif. The phosphorylated R-Smad dissociates from the receptor and forms a heteromeric complex with the co-Smad (Smad4), allowing translocation of the complex to the nucleus. Once in the nucleus, Smads can target a variety of DNA binding proteins to regulate transcriptional responses (6-8).

Smad信号转导分子家族成员是胞内通路中TGF-β信号从细胞表面传送到细胞核的重要组成部分。Smad 可被分为三类:受体调控型Smad(简称R-Smad),主要包括Smad1,2,3,5和8;共同介导型Smad(co-Smad)包括Smad4;还有拮抗型或称抑制型Smad(I-Smads),包括Smad6和Smad7[1-5]。激活的I型受体与特异的R-Smad有关,并能在R-Smad的保守的C端SSXS基序处对其磷酸化。磷酸化的R-Smad与受体分离,再与co-Smad(Smad4)形成异侧复合物,使复合物迁移入核。一旦进入核内,Smad可以各种DNA结合蛋白为目标调控转录反应。[6-8]

  1. Heldin, C.H. et al. (1997) Nature 390, 465-71.
  2. Attisano, L. and Wrana, J.L. (1998) Curr Opin Cell Biol 10, 188-94.
  3. Derynck, R. et al. (1998) Cell 95, 737-40.
  4. Massagué, J. (1998) Annu Rev Biochem 67, 753-91.
  5. Whitman, M. (1998) Genes Dev 12, 2445-62.
  6. Wu, G. et al. (2000) Science 287, 92-7.
  7. Attisano, L. and Wrana, J.L. (2002) Science 296, 1646-7.
  8. Moustakas, A. et al. (2001) J Cell Sci 114, 4359-69.

Application References

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Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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