Cell Signaling Technology

Product Pathways - Cell Cycle / Checkpoint

Phospho-cdc25C (Thr48) (D2H3) Rabbit mAb #12028

No. Size Price
12028S 100 µl ( 10 western blots ) ¥3,900.00 现货查询 购买询价
12028 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human, Endogenous 75 Rabbit IgG
IP 1:100
IF-IC 1:300

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation, IF-IC=Immunofluorescence (Immunocytochemistry),


Species predicted to react based on 100% sequence homology: Monkey,

Specificity / Sensitivity

Phospho-cdc25C (Thr48) (D2H3) Rabbit mAb recognizes endogenous levels of cdc25C protein only when phosphorylated at Thr48.Phospho-cdc25C (Thr48) (D2H3)Rabbit mAb可以识别48位苏氨酸磷酸化后的内源性cdc25C蛋白。

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Thr48 of human cdc25C protein.单克隆抗体由合成肽段免疫动物产生,该肽段与人cdc25C蛋白的48位苏氨酸邻近残基序列一致。



Confocal immunofluorescent analysis of HT-29 cells, untreated (left) or λ phosphatase-treated (right), using Phospho-cdc25C (Thr48) (D2H3) Rabbit mAb (green). Actin filaments were labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).使用Phospho-cdc25C (Thr48) (D2H3) Rabbit mAb (绿色)对未处理(左)或λ phosphatase处理(右)的HT-29细胞进行激光共聚焦荧光分析。肌动蛋白丝使用DY-554鬼笔环肽标记(红色)。蓝色假色= DRAQ5® #4084 (fluorescent DNA dye).

Western Blotting

Western Blotting

Western blot analysis of extracts from HT-29 cells, asynchronous (-) or synchronized in mitosis (thymidine block, nocodazole release, mitotic shake-off; +), using Phospho-cdc25C (Thr48) (D2H3) Rabbit mAb (upper) or cdc25C (5H9) Rabbit mAb #4688 (lower).对细胞周期不同(-)或同步(thymidine block, nocodazole release, mitotic shake-off; +)的HT-29细胞提取物,使用使用Phospho-cdc25C (Thr48) (D2H3) Rabbit mAb(上)或cdc25C(5H9)Rabbit Mab#4688(下)进行western blot分析。


Cdc25 is a protein phosphatase responsible for dephosphorylating and activating cdc2, a crucial step in regulating the entry of all eukaryotic cells into mitosis (1). cdc25C is constitutively phosphorylated at Ser216 throughout interphase by c-TAK1, while phosphorylation at this site is DNA damage-dependent at the G2/M checkpoint (2). When phosphorylated at Ser216, cdc25C binds to members of the 14-3-3 family of proteins, sequestering cdc25C in the cytoplasm and thereby preventing premature mitosis (3). The checkpoint kinases Chk1 and Chk2 phosphorylate cdc25C at Ser216 in response to DNA damage (4,5).Cdc25 是一种蛋白磷酸酯酶,能够去磷酸化激活cdc2,这是对于所有真核细胞进入有丝分裂期这关键一步的调控(1)。在有丝分裂间期,cdc25C的216位丝氨酸会一直被c-TAK1磷酸化,该位点的磷酸化在G2/M检验期依赖于DNA损伤(2)。当216位丝氨酸被磷酸化后,cdc25C可以和14-3-3家族蛋白结合,是cdc25C退回细胞质避免过早的有丝分裂(3)。检验点激酶Chk1和Chk2在DNA损伤后会磷酸化cdc25C的216位丝氨酸(4,5)。

Full activation of cdc25C involves phosphorylation at more than 12 different sites by cdc2/cyclin B and Polo-like kinase, and the activity of Pin1, a peptidyl-prolyl isomerase (PPI) (6,7). Pin1 contains a WW domain that binds phospho-Ser/Thr-Pro sites and a catalytic PPI region that induces a cis/trans isomerization at phospho-Ser/Thr-Pro bonds (8). Thr48 and Thr67 of cdc25C interact directly with the WW domain of Pin1 when these sites are phosphorylated (9). Thr48 phosphorylation also mediates binding to CKS/p13SUC1 (10).cdc25C的完全激活需要被cdc2/cyclin B和Polo-样激酶对其超过12个不同位点进行磷酸化,以及Pin1的活性,Pin1是一个肽脯氨酰异构酶 (PPI) (6,7). Pin1包括了1个WW结构域可以和磷酸化-丝氨酸/苏氨酸-脯氨酸位点结合以及一个催化PPI区域,该PPI区域可以诱导蛋白在磷酸化-丝氨酸/苏氨酸-脯氨酸位点形成顺/反异构体(8). cdc25C的48和67位苏氨酸被磷酸化后,cdc25C可以直接与Pin1的WW结构域结合(9)。48位苏氨酸磷酸化还会介导cdc25C与CKS/p13SUC1的结合 (10)。

  1. Jessus, C. and Ozon, R. (1995) Prog. Cell Cycle Res. 1, 215-228.
  2. Peng, C.Y. et al. (1997) Science 277, 1501-1505.
  3. Kumagai, A. and Dunphy, W.G. (1999) Genes Dev. 13, 1067-1072.
  4. Blasina, A. et al. (1999) Curr. Biol. 9, 1-10.
  5. Furnari, B. et al. (1999) Mol. Biol. Cell 10, 833-845.
  6. Izumi, T. and Maller, J.L. (1993) Mol Biol Cell 4, 1337-50.
  7. Stukenberg, P.T. and Kirschner, M.W. (2001) Mol Cell 7, 1071-83.
  8. Yaffe, M.B. et al. (1997) Science 278, 1957-60.
  9. Lu, P.J. et al. (1999) Science 283, 1325-8.
  10. Landrieu, I. et al. (2001) J Biol Chem 276, 1434-8.

Application References

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