Cell Signaling Technology

Product Pathways - TGF-beta/Smad Signaling

Smurf2 (D8B8) Rabbit mAb #12024

No. Size Price
12024S 100 µl ( 10 western blots ) ¥3,250.00 现货查询 购买询价 防伪查询
12024 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat,Monkey, Endogenous 80 Rabbit IgG
IP 1:50

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation,

Specificity / Sensitivity

Smurf2 (D8B8) Rabbit mAb recognizes endogenous levels of total Smurf2 protein. This antibody also cross-reacts with proteins of unknown origin at 250 and 46 kDa in some cell lines.

Smurf2 (D8B8)Rabbit mAb兔单抗可以识别内源性的总Smurf2蛋白。该抗体在某些细胞系中与未知来源的大约250和46kDa的蛋白有交叉反应。

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro160 of human Smurf2 protein.


Western Blotting

Western Blotting

Western blot analysis of extracts from U-2 OS and ACHN cells using Smurf2 (D8B8) Rabbit mAb.

使用Smurf2 (D8B8)Rabbit mAb对U-2OS和ACHN细胞提取物进行western blot分析。


Smad ubiquitin regulatory factor 2 (Smurf2) is a HECT domain E3 ubiquitin ligase. It was initially identified as an inhibitor of TGF-β/BMP signaling by targeting R-Smads and TGF type I receptor for ubiquitination and degradation (1-3). Subsequent studies have revealed its role in neuronal and planar cell polarity, as well as in the senescence response and suppression of tumorigenesis (4-8). Smurf2 has a broad range of substrates including RUNX2, AMSH, Rap1B, and RNF11 (5,9-11). Smurf2 is widely expressed in various tissues. The C2 domain of Smurf2 inhibits its catalytic activity by interacting with the HECT domain (12). Research studies have shown that Smurf2 functions as a tumor suppressor by maintaining genomic stability through targeting RNF20 (13).

Smad ubiquitin regulatory factor 2 (Smurf2)是一个HECT结构域E3泛素连接酶。最初被认为是TGF-β/BMP的抑制因子,靶向R-Smads和TGF I型受体进行泛素化降解(1-3)。随后的研究显示它在神经和平面细胞极性过程中,也在在衰老反应和抑制肿瘤发生过程中发挥作用(4-8)。Smurf2有多种底物包括RUNX2, AMSH, Rap1B,和RNF11 (5,9-11)。Smurf2在多种组织中表达。Smurf2的C2结构域与HECT结构与结合抑制其催化活性(12)。研究显示Smurf2作为抑癌因子靶向RNF20保持基因组稳定性(13)。

  1. Zhang, Y. et al. (2001) Proc Natl Acad Sci U S A 98, 974-9.
  2. Kavsak, P. et al. (2000) Mol Cell 6, 1365-75.
  3. Izzi, L. and Attisano, L. (2004) Oncogene 23, 2071-8.
  4. Zhang, H. and Cohen, S.N. (2004) Genes Dev 18, 3028-40.
  5. Schwamborn, J.C. et al. (2007) EMBO J 26, 1410-22.
  6. Narimatsu, M. et al. (2009) Cell 137, 295-307.
  7. Nie, J. et al. (2010) J Biol Chem 285, 22818-30.
  8. Ramkumar, C. et al. (2012) Cancer Res 72, 2714-9.
  9. Subramaniam, V. et al. (2003) Br J Cancer 89, 1538-44.
  10. Li, H. and Seth, A. (2004) Oncogene 23, 1801-8.
  11. Kaneki, H. et al. (2006) J Biol Chem 281, 4326-33.
  12. Wiesner, S. et al. (2007) Cell 130, 651-62.
  13. Blank, M. et al. (2012) Nat Med 18, 227-34.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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