Cell Signaling Technology

Product Pathways - Chromatin Regulation / Epigenetics

CHD4 (D4B7) Rabbit mAb #12011

No. Size Price
12011S 100 µl ( 10 western blots ) ¥3,100.00 现货查询 购买询价
12011 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat,Monkey, Endogenous 260 Rabbit IgG
IF-IC 1:400
ChIP 1:50

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IF-IC=Immunofluorescence (Immunocytochemistry), ChIP=Chromatin IP,

Homology

Species predicted to react based on 100% sequence homology: Hamster, Bovine, Pig, Guinea Pig, Horse,

Specificity / Sensitivity

CHD4 (D4B7) Rabbit mAb recognizes endogenous levels of total CHD4 protein. Based on sequence alignment, this antibody is not predicted to cross-react with other CHD proteins.

CHD4 (D4B7) Rabbit mAb兔单抗能够识别内源性CHD4总蛋白水平。基于序列信息分析,预测该抗体不与其他CHD蛋白发生交叉反应。

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro1533 of human CHD4 protein.

该单克隆抗体是采用合成的与人源CHD4蛋白Pro1533残基周围序列相对应的肽段免疫动物而生产的。

IF-IC

IF-IC

Confocal immunofluorescent analysis of 293T cells using CHD4 (D4B7) Rabbit mAb (green). Actin filaments were labeled with DY-554 phalloidin (red). 采用共聚焦免疫荧光术检测293T细胞,使用的抗体为CHD4 (D4B7) Rabbit mAb (绿色)。微丝结构利用DY-554 phalloidin(red)标记(红)。

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using CHD4 (D4B7) Rabbit mAb. Western blot检测各种细胞系提取物,使用抗体CHD4 (D4B7) Rabbit mAb。

Chromatin IP

Chromatin IP

Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 106 K562 cells and either 10 μl of CHD4 (D4B7) Rabbit mAb or 2 μl of Normal Rabbit IgG #2729 using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using SimpleChIP® Human GAPDH Exon 1 Primers #5516,

SimpleChIP® Human RPL30 Exon 3 Primers #7014, and SimpleChIP® Human MyoD1 Exon 1 Primers #4490. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.

Background

Chromodomain-helicase-DNA-binding domain (CHD) proteins have been identified in a variety of organisms (1,2). This family of nine proteins is divided into three separate subfamilies: subfamily I (CHD1 and CHD2), subfamily II (CHD3 and CHD4), and subfamily III (CHD5, CHD6, CHD7, CHD8, CHD9). All CHD proteins contain two tandem amino-terminal chromodomains, a SWI/SNF-related ATPase domain, and a carboxy-terminal DNA-binding domain (1,2). The chromodomains facilitate binding to methylated lysine residues of histone proteins and confer interactions with specific regions of chromatin. The SWI/SNF-related ATPase domain utilizes energy from ATP hydrolysis to modify chromatin structure. CHD proteins are often found in large, multiprotein complexes with their transcriptional activation or repression activity governed by other proteins within the complex. CHD3 (also known as Mi2-α) and CHD4 (also known as Mi2-β) are central components of the nucleosome remodeling and histone deacetylase (NuRD) transcriptional repressor complex, which also contains HDAC1, HDAC2, RBAP48, RBAP46, MTA1, MTA2, MTA3, and MBD3 (3-8). Both CHD3 and CHD4 contain two plant homeodomain (PHD) zinc finger domains that bind directly to HDAC1 and HDAC2.

染色质解旋酶DNA结合域(CHD)蛋白已经在各种生物组织中得到确认分析(1,2)。该蛋白家族具有九个蛋白,被分成三个亚家族:亚家族I(CHD1和CHD2)、亚家族II(CHD3和CHD4)、亚家族III(CHD5,CHD6,CHD7,CHD8,CHD9)。所有的CHD蛋白都具有串联的两个N端染色质结构域,一个SWI/SNF相关的ATP酶结构域和一个C端的DNA结合结构域(1,2)。N端的染色质结构域有助于结合组蛋白上甲基化的赖氨酸残基,加强染色质特殊区域的相互作用。SWI/SNF相关的ATP酶结构域利用ATP水解的能量对染色质结构进行改造。CHD蛋白通常是在一些大的多蛋白复合物中存在,CHD蛋白的转录激活或者其抑制活性受这些大的多蛋白复合物中其他蛋白影响。CHD3(又称Mi2-α)和CHD4 (也被称为Mi2-β)是核小体重塑和组蛋白脱乙酰酶(NuRD)转录抑制复合物的核心组成,该复合物也含有HDAC1, HDAC2, RBAP48, RBAP46, MTA1, MTA2, MTA3, 和 MBD3 (3-8)。CHD3和CHD4含有植物同源的(PHD)锌指结构域,直接与HDAC1和HDAC2相互作用。

  1. Hall, J.A. and Georgel, P.T. (2007) Biochem Cell Biol 85, 463-76.
  2. Marfella, C.G. and Imbalzano, A.N. (2007) Mutat Res 618, 30-40.
  3. Tong, J.K. et al. (1998) Nature 395, 917-21.
  4. Xue, Y. et al. (1998) Mol Cell 2, 851-61.
  5. Zhang, Y. et al. (1998) Cell 95, 279-89.
  6. Bowen, N.J. et al. (2004) Biochim Biophys Acta 1677, 52-7.
  7. Jones, P.L. et al. (1998) Nat Genet 19, 187-91.
  8. Fujita, N. et al. (2003) Cell 113, 207-19.

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Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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