Cell Signaling Technology

Product Pathways - Chromatin Regulation / Epigenetics

SSRP1 Antibody #12009


No. Size Price
12009S 100 µl ( 10 western blots ) ¥3,100.00 现货查询 购买询价
12009 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat,Monkey, Endogenous 81 Rabbit

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting,


Species predicted to react based on 100% sequence homology: Hamster, Bovine, Guinea Pig, Horse,

Specificity / Sensitivity

SSRP1 Antibody recognizes endogenous levels of total SSRP1 protein. This antibody also cross-reacts with a protein of unknown origin at 150 kDa.

SSRP1 Antibody能够检测内源性 SSRP1总蛋白水平。该抗体还会与一个150kDa的未知来源蛋白发生交叉反应。

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu148 of human SSRP1 protein. Antibodies are purified by protein A and peptide affinity chromatography.

该多克隆抗体是采用合成的与人源SSRP1残基周围序列相对应的肽段免疫动物而生产的。并经过Protein A和多肽亲和层析纯化。

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using SSRP1 Antibody. Western blot方法检测不同细胞系的提取物,使用的抗体为 SSRP1 Antibody。


Supressor of Ty-16 (SPT16) and structure-specific recognition protein-1 (SSRP1) are subunits of the facilitates chromatin transcription (FACT) complex that is essential for transcription elongation (1,2). FACT facilitates RNA polymerase-dependent transcription of chromatin templates by destabilizing the nucleosomes within the open reading frames of active genes (3-5). FACT destabilizes the nucleosomes, which would otherwise act as barriers to RNA polymerase transcription activity, by disrupting histone-histone and histone-DNA contacts that lead to the eviction of the histone H2A-H2B dimer (2,3,6). FACT may also function as a histone chaperone to reassemble nucleosomes after RNA polymerase passage (7). In addition to transcription, FACT activity has been shown to have a role in DNA replication in yeast and in DNA repair by contributing to the activation of p53 by CK2 and by facilitating histone H2AX-H2B exchange upon DNA damage (8-10).

Supressor of Ty-16 (SPT16)以及structure-specific recognition protein-1 (SSRP1)是转录延伸中至关重要的染色质转录促进复合物的亚单位(1,2)。FACT能够通过对活跃基因开放阅读框内核小体的去稳定作用来促进RNA聚合酶依赖的转录(3-5)。FACT能够破坏导致H2A-H2B二聚体丢失的组蛋白-组蛋白以及组蛋白-DNA的联系,从而使成为RNA聚合酶转录激活障碍的核小体去稳定(2,3,6)。FACT可能还可作为组蛋白伴侣在RNA聚合酶通路之后重新组装核小体(7)。除了转录,FACT还可以通过CK2促进p53活化以及促进DNA损伤的组蛋白H2AX-H2B交换,从而在酵母的DNA复制和DNA修复中发挥作用(8-10)。

  1. Winkler, D.D. and Luger, K. (2011) J Biol Chem 286, 18369-74.
  2. Orphanides, G. et al. (1999) Nature 400, 284-8.
  3. Orphanides, G. et al. (1998) Cell 92, 105-16.
  4. Birch, J.L. et al. (2009) EMBO J 28, 854-65.
  5. Orphanides, G. and Reinberg, D. (2000) Nature 407, 471-5.
  6. Keller, D.M. and Lu, H. (2002) J Biol Chem 277, 50206-13.
  7. Belotserkovskaya, R. et al. (2003) Science 301, 1090-3.
  8. Schlesinger, M.B. and Formosa, T. (2000) Genetics 155, 1593-606.
  9. Heo, K. et al. (2008) Mol Cell 30, 86-97.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

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