Cell Signaling Technology

Product Pathways - Vesicle Trafficking

Phospho-REPS1 (Ser709) (D8C1) Rabbit mAb #12005

No. Size Price
12005S 100 µl ( 10 western blots ) ¥4,050.00 现货查询 购买询价
12005 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Monkey, Endogenous 125 Rabbit IgG
IP 1:100

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation,

Specificity / Sensitivity

Phospho-REPS1 (Ser709) (D8C1) Rabbit mAb recognizes endogenous levels of REPS1 protein only when phosphorylated at Ser709.

Phospho-REPS1 (Ser709) (D8C1) Rabbit mAb可以识别Ser709被磷酸化的REPS1蛋白。

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser709 of human REPS1 protein.


Western Blotting

Western Blotting

Western blot analysis of extracts from Jurkat cells, untreated (-) or calf intestinal phosphatase (CIP)-treated (+), using Phospho-REPS1 (Ser709) (D8C1) Rabbit mAb (upper) or REPS1 (D6F4) Rabbit mAb #6404 (lower).未处理或经过牛小肠碱性磷酸酶(CIP)处理(+)的Jurkat细胞提取物,使用Phospho-REPS1 (Ser709) (D8C1) Rabbit mAb (上) 或 REPS1 (D6F4) Rabbit mAb #6404 (下)进行western blot分析。



Immunoprecipitation of phospho-REPS1 (Ser709) from Jurkat cell extracts, using Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) or Phospho-REPS1 (Ser709) (D8C1) Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using Phospho-REPS1 (Ser709) (D8C1) Rabbit mAb.使用 Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) 或Phospho-REPS1 (Ser709) (D8C1) Rabbit mAb (lane 3)对Jurkat细胞提取物中的phospho-REPS1 (Ser709)进行免疫沉淀实验。Lane1是10%上样量。使用Phospho-REPS1 (Ser709) (D8C1) Rabbit mAb进行western blot分析。


REPS1 is a RalBP1-associated EH-homology domain containing protein. The sequence of REPS1 has an EH domain, followed by two proline-rich segments, and a C-terminal coiled-coil domain for binding to RalBP1 (1). The EH domain of REPS1 interacts with the NPF motif of Rab11-FIP2, mediates their colocalization to endosome vesicles, and influences EGFR endocytosis (2). The two proline-rich regions of REPS1 are important for binding to the SH3 domain of GRK/GRB2 and further regulate EGFR downstream signaling. The proline-rich regions of REPS1 have also been shown to interact with the SH3 domain of intersectin1 (ITSN1) and contribute to ITSN1/SGIP1/REPS1 complex formation on clathrin-coated pits (3). Three alternatively spliced isoforms of REPS1 have been identified.

REPS1是一个含有RalBP1-associated EH同源结构域的蛋白。REPS1含有一个EH结构域,紧随其后的是2个富含脯氨酸的区域,以及一个C末端螺旋换结构域以结合RalBP1(1)。REPS1的EH结构域与Rab11-FIP2的NPF结构域结合,接到了他们共定位到核内体囊泡,影响EGFR内吞(2)。REPS1的两个富含脯氨酸的区域对于蛋白与GRK/GRB2的SH3结构域结合是非常重要的而且能够调控EGFR下游的信号通路。RESP1富含脯氨酸的区域也被认为可以和intersectin1 (ITSN1)的SH3结构域结合,引起网格蛋白小窝形成ITSN1/SGIP1/REPS1复合物(3)。REPS1发现了3中剪切异构体。

Phosphorylation of REPS1 at Ser709 was identified at Cell Signaling Technology using PTMScan® Technology, our LC-MS/MS platform for phosphorylation site discovery (4).

Cell Signaling Technology PTMScan®技术,发现磷酸化修饰位点的LC-MS/MS平台,发现了REPS1的Ser709磷酸化。

  1. Yamaguchi, A. et al. (1997) J Biol Chem 272, 31230-4.
  2. Cullis, D.N. et al. (2002) J Biol Chem 277, 49158-66.
  3. Dergai, O. et al. (2010) Biochem Biophys Res Commun 402, 408-13.
  4. Rush, J. et al. (2005) Nat Biotechnol 23, 94-101.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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