Cell Signaling Technology

Product Pathways - Metabolism

SDHA (D6J9M) XP® Rabbit mAb  #11998

1500032O14Rik   2310034D06Rik   4921513A11   C81073   DHSA   Flavoprotein subunit of complex II   Fp   sc-377302   SDH2   Sdha   SDHF   succinate dehydrogenase complex. subunit A. flavoprotein (Fp)   Succinate dehydrogenase [ubiquinone] flavoprotein subunit. mitochondrial  

No. Size Price
11998S 100 µl ( 10 western blots ) ¥3,580.00 现货查询 购买询价
11998T 20 µl ( 2 western blots ) ¥1,400.00 现货查询 购买询价
11998 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat,Hamster,Monkey, Endogenous 70 Rabbit IgG
IP 1:50
IHC-P 1:200
IF-IC 1:100

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation, IHC-P=Immunohistochemistry (Paraffin), IF-IC=Immunofluorescence (Immunocytochemistry),

Specificity / Sensitivity

SDHA (D6J9M) XP® Rabbit mAb recognizes endogenous levels of total SDHA protein.

SDHA (D6J9M) XP® Rabbit mAb能够检测内源性SDHA总蛋白水平。

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly166 of human SDHA protein.




Confocal immunofluorescent analysis of HeLa cells using SDHA (D6J9M) XP® Rabbit mAb (green) and β-Actin (8H10D10) Mouse mAb #3700 (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

共聚焦免疫荧光分析293细胞,使用抗体为SDHA (D6J9M) XP® Rabbit mAb兔单抗(绿色)和β-Actin (8H10D10) Mouse mAb鼠单抗#3700(红色)。蓝色伪彩=DRAQ5®#4084(荧光DNA染料)。

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using SDHA (D6J9M) XP® Rabbit mAb.

Wetern blot检测多种细胞系提取物,所用抗体为SDHA (D6J9M) XP® Rabbit mAb兔单抗。



Immunoprecipitation of SDHA from HeLa cell extracts using Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) or SDHA (D6J9M) XP® Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using SDHA (D6J9M) XP® Rabbit mAb.

免疫沉淀方法检测HeLa细胞提取物SDHA蛋白,使用抗体为Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (泳道2)或SDHA (D6J9M) XP® Rabbit mAb兔单抗 (泳道3)。泳道1为10%对照。使用SDHA (D6J9M) XP® Rabbit mAb兔单抗 进行Western blot分析。

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human colon carcinoma using SDHA (D6J9M) XP® Rabbit mAb in the presence of control peptide (left) or antigen-specific peptide (right).

免疫组织化学分析石蜡包埋的人结肠癌切片,使用抗体为SDHA (D6J9M) XP® Rabbit mAb兔单抗,对照(左)或抗原特异性肽(右)。


Succinate dehydrogenase (SDH), also known as Complex II or succinate:quinone oxidoreductase, is a key component of the citric acid cycle and the electron transport chain (1). Specifically, it is involved in the oxidation of succinate (2). SDH consists of four subunits: SDHA, SDHB, SDHC, and SDHD (3). Research studies have shown that defects in SDHA cause complex II deficiency (2). In addition, investigators have observed reduction of SDHA in the striatum of patients with Huntington’s disease (3), and reduction of SDHB, SDHC, and SDHD in paragangliomas and phenochromocytomas(4,5).


  1. Oyedotun, K.S. and Lemire, B.D. (2004) J Biol Chem 279, 9424-31.
  2. Bourgeron, T. et al. (1995) Nat Genet 11, 144-9.
  3. Benchoua, A. et al. (2006) Mol Biol Cell 17, 1652-63.
  4. Baysal, B.E. et al. (2000) Science 287, 848-51.
  5. Feichtinger, R.G. et al. (2010) BMC Cancer 10, 149.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

DRAQ5 is a registered trademark of Biostatus Limited.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

XP is a registered trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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